Evidence map›Paper›PMID 39272153›Full record

ArticleBMC veterinary research2024

Characterization of giant endocrine cells in the fundic stomach of African catfish (Clarias gariepinus) demonstrated by histochemical, immunohistochemical and ultrastructure microscopy methods suggesting their role in immunity.

Hanan H Abd-El-Hafeez, Sulaiman Mohammed Alnasser, Zyad M Baker, Mohamed Aref, Mohamed A M Alsafy, Samir A A El-Gendy, Eman Zahran, Hams Mohamed M A, Ali H Alghamdi, Mahmoud Osman Khalifa and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Hanan H Abd-El-HafeezDepartment of Cell and Tissues, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt. hhmmzz91@gmail.com.ORCID https://orcid.org/0000-0002-2547-0709
Sulaiman Mohammed AlnasserDepartment of Pharmacology and Toxicology, College of Pharmacy, Qassim University, Qassim, 51452, Saudi Arabia.
Zyad M BakerFaculty of Medicine, Assiut University, Assiut, Egypt.
Mohamed ArefDepartment of Anatomy and Embryology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44519, Egypt.
Mohamed A M AlsafyAnatomy and Embryology Department, Faculty of Veterinary Medicine, Alexandria University, Abis 10th, P.O. 21944, Alexandria, Egypt.
Samir A A El-GendyAnatomy and Embryology Department, Faculty of Veterinary Medicine, Alexandria University, Abis 10th, P.O. 21944, Alexandria, Egypt.
Eman ZahranDepartment of Aquatic Animal Medicine, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
Hams Mohamed M ADepartment of Microbiology, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt.
Ali H AlghamdiDepartment of Biology, Faculty of Science, Al-Baha University, Alaqiq, Saudi Arabia.ORCID https://orcid.org/0000-0003-0426-9237
Mahmoud Osman KhalifaDepartment of Anatomy and Embryology, Faculty of Veterinary Medicine, Aswan University, Aswan, Egypt.
Basma M KamalDepartment of Anatomy and Embryology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, 6010230, Egypt.
Fawzyah A AlghamdiDepartment of Biological Science, College of Science, University of Jeddah, P.O. Box 80327, 21589, Jeddah, Saudi Arabia.
Soha A SolimanDepartment of Histology, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt. soha_soliman@vet.svu.edu.eg.
Diaa MassoudDepartment of Zoology, Faculty of Science, Fayoum University, Fayoum, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Endocrine cells in the fundic stomach of Clarias gariepinus were characterized in this work using transmission electron microscopy, immunohistochemistry, and histochemistry. Performic acid mixed with alcian blue pH2.5 and silver stain were among the histochemical stains used for endocrine cells. Endocrine cells can be found in the epithelium, lamina propria, submucosa, muscular layer, serosa, and the area between the stomach glands. Endocrine cells with one or more nuclei were found. Endocrine cells were studied using CD3, CD21, and CD68 in an immunohistochemistry analysis. The expression of the lymphocyte marker CD3 by endocrine cells is remarkable. In addition, they had a strong immunological response to CD21 and CD68, which are characteristics of phagocytic cells. Granules of varied sizes and electron densities are packed densely into the cytoplasm of the cells, as seen by transmission electron microscopy. We propose that endocrine cells play a crucial role in immune defense. The role of endocrine cells in the gut's immune system is an area that needs further investigation.

Indexed as

CatfishesImmunohistochemistryMicroscopy, Electron, TransmissionAnimalsEndocrine CellsGiant CellsStomachCatfishCD21CD3CD68Cellular-mediated reactionHistochemicalTEM

Identifiers

PMID39272153
PMCPMC11401327

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.