Evidence map›Paper›PMID 39273092›Full record

ArticleInternational journal of molecular sciences2024

Electroporation Delivery of Cas9 sgRNA Ribonucleoprotein-Mediated Genome Editing in Sheep IVF Zygotes.

Wenhui Pi, Guangyu Feng, Minghui Liu, Cunxi Nie, Cheng Chen, Jingjing Wang, Limin Wang, Pengcheng Wan, Changbin Liu, Yi Liu and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Engineering Immune Cell to Counteract Aging and Aging-Associated Diseases.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. CRISPR-Cas Systems: A Functional Perspective and Innovations.International journal of molecular sciences · 2025
    Review
  8. Article
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Wenhui PiCollege of Animal Science and Technology, Shihezi University, Shihezi 832003, China.
Guangyu FengCollege of Animal Science and Technology, Shihezi University, Shihezi 832003, China.
Minghui LiuCollege of Animal Science and Technology, Shihezi University, Shihezi 832003, China.
Cunxi NieCollege of Animal Science and Technology, Shihezi University, Shihezi 832003, China.ORCID 0000-0003-3320-2840
Cheng ChenCollege of Animal Science and Technology, Shihezi University, Shihezi 832003, China.ORCID 0000-0002-0242-9332
Jingjing WangState Key Laboratory of Sheep Genetic Improvement and Healthy Breeding, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China.
Limin WangState Key Laboratory of Sheep Genetic Improvement and Healthy Breeding, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China.
Pengcheng WanState Key Laboratory of Sheep Genetic Improvement and Healthy Breeding, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China.
Changbin LiuState Key Laboratory of Sheep Genetic Improvement and Healthy Breeding, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China.
Yi LiuState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, China.
Ping ZhouState Key Laboratory of Sheep Genetic Improvement and Healthy Breeding, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi 832000, China.

Funding

Bingtuan Science and Technology Project NYHXGG2023AA101Research Training Program for Undergraduates of Shihezi University SRP2024014Science and Technology Specialist Team Service Program of Shihezi University KJTP202401Startup Foundation for Advanced Talents of Shihezi university RCZK2021B34
6 · The paper itself

Abstract

The utilization of electroporation for delivering CRISPR/Cas9 system components has enabled efficient gene editing in mammalian zygotes, facilitating the development of genome-edited animals. In this study, our research focused on targeting the

Indexed as

CRISPR-Cas SystemsElectroporationFertilization in VitroGene EditingRibonucleoproteinsRNA, Guide, CRISPR-Cas SystemsZygoteAnimalsAnimals, Genetically ModifiedCRISPR-Associated Protein 9FemaleMyostatinSheepCRISPR-Associated Protein 9MyostatinRibonucleoproteinsRNA, Guide, CRISPR-Cas SystemsCRISPR/Cas9electroporationgene editinghomologous recombinationin vitro fertilization (IVF)mutation efficiencysheep zygotes

Identifiers

PMID39273092
PMCPMC11395511

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.