Evidence map›Paper›PMID 39276351›Full record

ArticleCell reports2024

Diribonuclease activity eliminates toxic diribonucleotide accumulation.

Soo-Kyoung Kim, Mona W Orr, Husan Turdiev, Conor C Jenkins, Justin D Lormand, Tanner M Myers, Audrey Andy Burnim, Jared A Carter, Warren C Kung, Xiaofang Jiang and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Soo-Kyoung KimDepartment of Cell Biology and Molecular Genetics, University of Maryland at College Park, College Park, MD 20742, USA; Research Institute for Drug Development, Pusan National University, Busan 46241, South Korea.
Mona W OrrDepartment of Cell Biology and Molecular Genetics, University of Maryland at College Park, College Park, MD 20742, USA.
Husan TurdievDepartment of Cell Biology and Molecular Genetics, University of Maryland at College Park, College Park, MD 20742, USA.
Conor C JenkinsDepartment of Cell Biology and Molecular Genetics, University of Maryland at College Park, College Park, MD 20742, USA.
Justin D LormandCSSB Centre for Structural Systems Biology, Deutsches Elektronen-Synchrotron DESY, 22607 Hamburg, Germany.
Tanner M MyersDepartment of Cell Biology and Molecular Genetics, University of Maryland at College Park, College Park, MD 20742, USA.
Audrey Andy BurnimIntramural Research Program, NLM, NIH, Bethesda, MD 20894, USA.
Jared A CarterDepartment of Cell Biology and Molecular Genetics, University of Maryland at College Park, College Park, MD 20742, USA.
Warren C KungDepartment of Cell Biology and Molecular Genetics, University of Maryland at College Park, College Park, MD 20742, USA.
Xiaofang JiangIntramural Research Program, NLM, NIH, Bethesda, MD 20894, USA.
Holger SondermannCSSB Centre for Structural Systems Biology, Deutsches Elektronen-Synchrotron DESY, 22607 Hamburg, Germany.
Wade C WinklerDepartment of Cell Biology and Molecular Genetics, University of Maryland at College Park, College Park, MD 20742, USA.
Vincent T LeeDepartment of Cell Biology and Molecular Genetics, University of Maryland at College Park, College Park, MD 20742, USA. Electronic address: vtlee@umd.edu.

Funding

Oligoribonuclease regulation of cyclic-di-GMP signaling and chronic biofilm infectionsR01AI142400 · NIAID · UNIV OF MARYLAND, COLLEGE PARK · PI LEE, VINCENT T · 2019 to 2023
$2.9M
Training in Host-pathogens interactionsT32AI089621 · NIAID · UNIV OF MARYLAND, COLLEGE PARK · PI LEE, VINCENT T, MCIVER, KEVIN S. · 2010 to 2025
$2.1M
NIAID NIH HHS R01 AI142400NIAID NIH HHS T32 AI089621
6 · The paper itself

Abstract

RNA degradation is a central process required for transcriptional regulation. Eventually, this process degrades diribonucleotides into mononucleotides by specific diribonucleases. In Escherichia coli, oligoribonuclease (Orn) serves this function and is unique as the only essential exoribonuclease. Yet, related organisms, such as Pseudomonas aeruginosa, display a growth defect but are viable without Orn, contesting its essentiality. Here, we take advantage of P. aeruginosa orn mutants to screen for suppressors that restore colony morphology and identified yciV. Purified YciV (RNase AM) exhibits diribonuclease activity. While RNase AM is present in all γ-proteobacteria, phylogenetic analysis reveals differences that map to the active site. RNase AM

Indexed as

ExoribonucleasesBacterial ProteinsEscherichia coliMutationPhylogenyPseudomonas aeruginosaRNA StabilityBacterial ProteinsExoribonucleasesoligoribonucleaseCP: Molecular biologydiribonucleasediribonucleotidesessentialitylinearoligoribonucleaseRNA degradationRNase AMYciV

Identifiers

PMID39276351
PMCPMC11528712

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.