Evidence map›Paper›PMID 39276355›Full record

ArticleSTAR protocols2024

STARDUST: A pipeline for the unbiased analysis of astrocyte regional calcium dynamics.

Yifan Wu, Yanchao Dai, Katheryn B Lefton, Timothy E Holy, Thomas Papouin

Abstract read
In one paragraph

Article in STAR protocols, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Ventral striatal astrocytes contribute to reinforcement learning.bioRxiv : the preprint server for biology · 2025
    Article
  5. Review
  6. Article
  7. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Yifan WuDepartment of Neuroscience, Washington University School of Medicine, St. Louis, MO 63110, USA.
Yanchao DaiDepartment of Neuroscience, Washington University School of Medicine, St. Louis, MO 63110, USA.
Katheryn B LeftonDepartment of Neuroscience, Washington University School of Medicine, St. Louis, MO 63110, USA.
Timothy E HolyDepartment of Neuroscience, Washington University School of Medicine, St. Louis, MO 63110, USA.
Thomas PapouinDepartment of Neuroscience, Washington University School of Medicine, St. Louis, MO 63110, USA. Electronic address: thomas.papouin@wustl.edu.

Funding

Role of astrocyte-based cholinergic neuromodulation in cognition and in the treatment of cognitive disordersR01MH127163 · NIMH · WASHINGTON UNIVERSITY · PI PAPOUIN, THOMAS · 2021 to 2025
$1.9M
NIMH NIH HHS R01 MH127163
6 · The paper itself

Abstract

Calcium imaging has become a popular way to probe astrocyte activity, but few techniques holistically capture discrete calcium signals occurring across the astrocyte domain. Here, we introduce STARDUST, a pipeline for the spatio-temporal analysis of regional dynamics and unbiased sorting of transients from fluorescence recordings of astrocytes. We describe steps for installing software, detecting active pixel patches, obtaining region of activity (ROA) maps, and extracting time series from ROAs. We then detail procedures for extracting signal features using custom-made code.

Indexed as

AstrocytesCalciumCalcium SignalingSoftwareAnimalsImage Processing, Computer-AssistedMiceSpatio-Temporal AnalysisCalciumCell BiologyNeuroscienceSignal Transduction

Identifiers

PMID39276355
PMCPMC11417172

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.