Evidence map›Paper›PMID 39278917›Full record

ArticleBMC biology2024

Paternal undernutrition and overnutrition modify semen composition and preimplantation embryo developmental kinetics in mice.

Hannah L Morgan, Nader Eid, Nadine Holmes, Sonal Henson, Victoria Wright, Clare Coveney, Catherine Winder, Donna M O'Neil, Warwick B Dunn, David J Boocock and 1 more

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Article in BMC biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Hannah L MorganLifespan and Population Health, School of Medicine, University of Nottingham, Nottingham, NG7 2UH, UK.
Nader EidLifespan and Population Health, School of Medicine, University of Nottingham, Nottingham, NG7 2UH, UK.
Nadine HolmesDeep Seq, School of Life Sciences, Queen's Medical Centre, University of Nottingham, Nottingham, NG7 2UH, UK.
Sonal HensonDeep Seq, School of Life Sciences, Queen's Medical Centre, University of Nottingham, Nottingham, NG7 2UH, UK.
Victoria WrightDeep Seq, School of Life Sciences, Queen's Medical Centre, University of Nottingham, Nottingham, NG7 2UH, UK.
Clare CoveneyThe John Van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, NG11 8NS, UK.
Catherine WinderPhenome Centre, School of Biosciences, University of Birmingham, Birmingham, B15 2TT, UK.
Donna M O'NeilPhenome Centre, School of Biosciences, University of Birmingham, Birmingham, B15 2TT, UK.
Warwick B DunnPhenome Centre, School of Biosciences, University of Birmingham, Birmingham, B15 2TT, UK.
David J BoocockThe John Van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, NG11 8NS, UK.
Adam J WatkinsLifespan and Population Health, School of Medicine, University of Nottingham, Nottingham, NG7 2UH, UK. adam.watkins@nottingham.ac.uk.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe importance of parental diet in relation to eventual offspring health is increasing in prominence due to the increased frequency of parents of reproductive age consuming poor diets. Whilst maternal health and offspring outcome have been studied in some detail, the paternal impacts are not as well understood. A father's poor nutritional status has been shown to have negative consequences on foetal growth and development and ultimately impact the long-term adult health of the offspring. In this study, we examined sperm- and seminal vesicle fluid-mediated mechanisms of preimplantation embryo development alterations in response to sub-optimal paternal diets.

resultsMale mice were fed a diet to model either under (low-protein diet (LPD)) or over (high-fat/sugar 'Western' diet (WD)) nutrition, LPD or WD supplemented with methyl donors or a control diet (CD) before mating with age-matched females. Male metabolic health was influenced by WD and MD-WD, with significant changes in multiple serum lipid classes and hepatic 1-carbon metabolites. Sperm RNA sequencing revealed significant changes to mRNA profiles in all groups when compared to CD (LPD: 32, MD-LPD: 17, WD: 53, MD-WD: 35 transcripts). Separate analysis of the seminal vesicle fluid proteome revealed a significant number of differentially expressed proteins in all groups (LPD: 13, MD-LPD: 27, WD: 24, MD-WD: 19) when compared to control. Following mating, in vitro time-lapse imaging of preimplantation embryos revealed a significant increase in the timing of development in all experimental groups when compared to CD embryos. Finally, qPCR analysis of uterine tissue at the time of implantation identified perturbed expression of Cd14 and Ptgs1 following mating with WD-fed males.

conclusionsOur current study shows that paternal nutritional status has the potential to influence male metabolic and reproductive health, impacting on embryonic development and the maternal reproductive tract. This study highlights potential direct (sperm-mediated) and indirect (seminal vesicle fluid-mediated) pathways in which a father's poor diet could shape the long-term health of his offspring.

Indexed as

Embryonic DevelopmentOvernutritionSemenAnimalsBlastocystFemaleMaleMalnutritionMiceSpermatozoaEmbryo developmentPaternal dietSemen profileSperm RNAUterine response

Identifiers

PMID39278917
PMCPMC11403970

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.