Evidence map›Paper›PMID 39282319›Full record

ArticlebioRxiv : the preprint server for biology2024

Design of linked-domain protein inhibitors of UBE2D as tools to study cellular ubiquitination.

Zara Bukhari, Li Gu, Anneroos E Nederstigt, Logan J Cope, Derek L Bolhuis, Kim Harvey, Tristan Allen, Spencer Hill, Yujie Yang, Guy Lawson and 14 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

24 authors.

Zara BukhariThe University of the Pacific, Department of Chemistry, Stockton, CA, 95210, USA.
Li GuThe University of the Pacific, Department of Chemistry, Stockton, CA, 95210, USA.ORCID 0000-0001-7044-6444
Anneroos E NederstigtThe University of the Pacific, Department of Chemistry, Stockton, CA, 95210, USA.ORCID 0000-0002-3695-3677
Logan J CopeThe University of the Pacific, Department of Chemistry, Stockton, CA, 95210, USA.
Derek L BolhuisDepartment of Pharmacology and Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, NC, USA.ORCID 0000-0002-9062-9739
Kim HarveyThe University of the Pacific, Department of Chemistry, Stockton, CA, 95210, USA.
Tristan AllenThe University of the Pacific, Department of Chemistry, Stockton, CA, 95210, USA.
Spencer HillDepartment of Chemistry and Biochemistry, University of Nevada, Las Vegas, Las Vegas, NV, USA.
Yujie YangDepartment of Pharmaceutics & Medicinal Chemistry, Thomas J. Long School of Pharmacy, University of the Pacific, Stockton, CA 95211, USA.
Guy LawsonThe University of the Pacific, Department of Chemistry, Stockton, CA, 95210, USA.
Cai LuThe University of the Pacific, Department of Chemistry, Stockton, CA, 95210, USA.
Tommy TranThe University of the Pacific, Department of Chemistry, Stockton, CA, 95210, USA.
Leah PinedaThe University of the Pacific, Department of Chemistry, Stockton, CA, 95210, USA.
Leanne LowThe University of the Pacific, Department of Chemistry, Stockton, CA, 95210, USA.
Andrew ChiangThe University of the Pacific, Department of Chemistry, Stockton, CA, 95210, USA.
Jason SongThe University of the Pacific, Department of Chemistry, Stockton, CA, 95210, USA.
Michelle V FongThe University of the Pacific, Department of Chemistry, Stockton, CA, 95210, USA.
Vanessa M RangelThe University of the Pacific, Department of Chemistry, Stockton, CA, 95210, USA.
William K ChanDepartment of Chemistry and Biochemistry, University of Nevada, Las Vegas, Las Vegas, NV, USA.
Gary KleigerDepartment of Chemistry and Biochemistry, University of Nevada, Las Vegas, Las Vegas, NV, USA.ORCID 0000-0003-3924-1680
Dennis GoldfarbDepartment of Cell Biology and Physiology, Institute for Informatics, Washington University, St. Louis, MO, USA.ORCID 0000-0002-1584-5423
Craig A VierraBiological Sciences Department, University of the Pacific, Stockton, CA 95211, USA.
Nicholas G BrownDepartment of Pharmacology and Lineberger Comprehensive Cancer Center, University of North Carolina School of Medicine, Chapel Hill, NC, USA.ORCID 0000-0002-6141-0164
Joseph S HarrisonThe University of the Pacific, Department of Chemistry, Stockton, CA, 95210, USA.ORCID 0000-0002-2118-6524

Funding

MOLECULAR AND CELLULAR BIOPHYSICS TRAINING PROGRAMT32GM008570 · NIGMS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI KUHLMAN, BRIAN A, SLEP, KEVIN C · 1995 to 2022
$5.1M
Spindle Assembly Checkpoint SilencingR35GM128855 · NIGMS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Nicholas Gene Brown · 2018 to 2026
$4.1M
How ubiquitin-carrying enzymes contribute to ubiquitin ligase specificityR01GM141409 · NIGMS · UNIVERSITY OF NEVADA LAS VEGAS · PI KLEIGER, GARY L. · 2021 to 2024
$1.6M
NIGMS NIH HHS R01 GM141409NIGMS NIH HHS R35 GM128855NIGMS NIH HHS T32 GM008570
6 · The paper itself

Abstract

Ubiquitin (Ub) is a post-translational modification that largely controls proteostasis through mechanisms spanning transcription, translation, and notably, protein degradation. Ub conjugation occurs through a hierarchical cascade of three enzyme classes (E1, E2, and E3s) involving >1000 proteins that regulate the ubiquitination of proteins. The E2 Ub-conjugating enzymes are the midpoint, yet their cellular roles remain under-characterized, partly due to a lack of inhibitors. For example, the cellular roles of the promiscuous E2 UBE2D/UBCH5 are not well described. Here, we develop a highly selective, multivalent, engineered protein inhibitor for the UBE2D family that simultaneously targets the RING- and backside-binding sites. In HeLa cells, these inhibitors phenocopy knockdown of UBE2D by reducing the IC

Identifiers

PMID39282319
PMCPMC11398408

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.