Evidence mapPaperPMID 39287634Full record

ArticleCellular and molecular life sciences : CMLS2024

Cytosolic Hmgb1 accumulation in mesangial cells aggravates diabetic kidney disease progression via NFκB signaling pathway.

Keqian Wu, He Zha, Tianhui Wu, Handeng Liu, Rui Peng, Ziyue Lin, Dan Lv, Xiaohui Liao, Yan Sun, Zheng Zhang

Abstract read
In one paragraph

Article in Cellular and molecular life sciences : CMLS, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed, 1 pooled it
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Review
  4. Review
  5. Article
  6. Article
  7. Review
  8. Review
  9. Article
  10. Review
  11. Review
  12. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Keqian Wu *Department of Nephrology, The Second Affiliated Hospital, Basic Medicine College, Key Laboratory of Major Brain Disease and Aging Research(Ministry of Education), Chongqing Medical University, Chongqing, 400010, China.
He Zha *Department of Laboratory Medicine, The First People's Hospital of Zunyi (The Third Affiliated Hospital of Zunyi Medical University), Zunyi, 563000, Guizhou, China.
Tianhui Wu *Department of Nephrology, The Second Affiliated Hospital, Basic Medicine College, Key Laboratory of Major Brain Disease and Aging Research(Ministry of Education), Chongqing Medical University, Chongqing, 400010, China.
Handeng LiuLaboratory of Tissue and Cell Biology, Experimental Teaching and Management Center, Chongqing Medical University, Chongqing, 400016, China.
Rui PengDepartment of Bioinformatics, Chongqing Medical University, Chongqing, 400016, China.
Ziyue LinDepartment of Nephrology, The Second Affiliated Hospital, Basic Medicine College, Key Laboratory of Major Brain Disease and Aging Research(Ministry of Education), Chongqing Medical University, Chongqing, 400010, China.
Dan LvDepartment of Nephrology, The Second Affiliated Hospital, Basic Medicine College, Key Laboratory of Major Brain Disease and Aging Research(Ministry of Education), Chongqing Medical University, Chongqing, 400010, China.
Xiaohui LiaoDepartment of Nephrology, The Second Affiliated Hospital, Basic Medicine College, Key Laboratory of Major Brain Disease and Aging Research(Ministry of Education), Chongqing Medical University, Chongqing, 400010, China. lxh@hospital.cqmu.edu.cn.
Yan SunDepartment of Nephrology, The Second Affiliated Hospital, Basic Medicine College, Key Laboratory of Major Brain Disease and Aging Research(Ministry of Education), Chongqing Medical University, Chongqing, 400010, China. yansun@cqmu.edu.cn.
Zheng ZhangDepartment of Nephrology, The Second Affiliated Hospital, Basic Medicine College, Key Laboratory of Major Brain Disease and Aging Research(Ministry of Education), Chongqing Medical University, Chongqing, 400010, China. zhangzheng@cqmu.edu.cn.ORCID http://orcid.org/0000-0002-0062-517X

Funding

National Natural Science Foundation of China 82270876
6 · The paper itself

Abstract

Diabetic kidney disease (DKD) is the predominant type of end-stage renal disease. Increasing evidence suggests thatglomerular mesangial cell (MC) inflammation is pivotal for cell proliferation and DKD progression. However, the exactmechanism of MC inflammation remains largely unknown. This study aims to elucidate the role of inflammatoryfactor high-mobility group box 1 (Hmgb1) in DKD. Inflammatory factors related to DKD progression are screened viaRNA sequencing (RNA-seq). In vivo and in vitro experiments, including db/db diabetic mice model, CCK-8 assay, EdUassay, flow cytometric analysis, Co-IP, FISH, qRT-PCR, western blot, single cell nuclear RNA sequencing (snRNA-seq),are performed to investigate the effects of Hmgb1 on the inflammatory behavior of MCs in DKD. Here, wedemonstrate that Hmgb1 is significantly upregulated in renal tissues of DKD mice and mesangial cells cultured withhigh glucose, and Hmgb1 cytopasmic accumulation promotes MC inflammation and proliferation. Mechanistically,Hmgb1 cytopasmic accumulation is two-way regulated by MC-specific cyto-lncRNA E130307A14Rik interaction andlactate-mediated acetylated and lactylated Hmgb1 nucleocytoplasmic translocation, and accelerates NFκB signalingpathway activation via directly binding to IκBα. Together, this work reveals the promoting role of Hmgb1 on MCinflammation and proliferation in DKD and helps expound the regulation of Hmgb1 cytopasmic accumulation in twoways. In particular, Hmgb1 may be a promising therapeutic target for DKD.

Indexed as

Diabetic NephropathiesHMGB1 ProteinMesangial CellsNF-kappa BSignal TransductionAnimalsCell ProliferationCytosolDiabetes Mellitus, ExperimentalDisease ProgressionHumansInflammationMaleMiceMice, Inbred C57BLHMGB1 ProteinHMGB1 protein, mouseNF-kappa BCytopasmic accumulationDiabetic kidney diseaseHmgb1Mesangial cellsNF-κB

Identifiers

PMID39287634
PMCPMC11408458

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.