Evidence mapPaperPMID 39289582Full record

ArticleNature cell biology2024

Plasma membrane curvature regulates the formation of contacts with the endoplasmic reticulum.

Yang Yang, Luis A Valencia, Chih-Hao Lu, Melissa L Nakamoto, Ching-Ting Tsai, Chun Liu, Huaxiao Yang, Wei Zhang, Zeinab Jahed, Wan-Ru Lee and 4 more

Erratum issuedAbstract read
In one paragraph

Article in Nature cell biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. Article
  2. Review
  3. Orai1 is required for CabioRxiv : the preprint server for biology · 2026
    Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Review
  10. Mechanobiological Dynamics-Inspired Mechanomodulatory Biomaterials.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Review
  11. Frontiers in cell and developmental biology · 2026
    Review
  12. Article
  13. Review
  14. Inter-Organellar CaCells · 2025
    Review
  15. Article
  16. Article
  17. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors.

Yang YangDepartment of Chemistry, Stanford University, Stanford, CA, USA.
Luis A ValenciaDepartment of Chemistry, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0003-1397-0308
Chih-Hao LuDepartment of Chemistry, Stanford University, Stanford, CA, USA.
Melissa L NakamotoDepartment of Chemistry, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0003-0955-6710
Ching-Ting TsaiDepartment of Chemistry, Stanford University, Stanford, CA, USA.
Chun LiuStanford Cardiovascular Institute, Stanford University, Stanford, CA, USA.
Huaxiao YangStanford Cardiovascular Institute, Stanford University, Stanford, CA, USA.
Wei ZhangDepartment of Chemistry, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0003-2285-3971
Zeinab JahedDepartment of Chemistry, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0002-0139-3219
Wan-Ru LeeDepartment of Physiology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Francesca SantoroTissue Electronics, Istituto Italiano di Tecnologia, Naples, Italy.
Jen LiouDepartment of Physiology, University of Texas Southwestern Medical Center, Dallas, TX, USA.ORCID http://orcid.org/0000-0003-1546-3115
Joseph C WuStanford Cardiovascular Institute, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0002-6068-8041
Bianxiao CuiDepartment of Chemistry, Stanford University, Stanford, CA, USA. bcui@stanford.edu.ORCID http://orcid.org/0000-0002-8044-5629

Funding

Elucidating Anthracycline-Induced Cell Type-Specific Cardiovascular Toxicity with CRISPRi/a ScreensR01HL150693 · STANFORD UNIVERSITY · 2025 to 2025
$770k
Human Induced Pluripotent Stem Cells for Cardiovascular Disease ModelingR01HL113006 · STANFORD UNIVERSITY · 2025 to 2025
$675k
Nanoscale probes for sensing molecular functions in live cellsR35GM141598 · STANFORD UNIVERSITY · 2025 to 2025
$637k
Single-cell Multi-omic Profiling of Drug Responses Using Pooled iPSC-CM DifferentiationR01HL145676 · STANFORD UNIVERSITY · 2025 to 2025
$600k
An electrophysiology platform that enables robust, scalable and long-term intracellular recording of cardiomyocytesR01HL165491 · STANFORD UNIVERSITY · 2025 to 2025
$589k
Stanford ChEM-H Chemistry/Biology Interface Predoctoral Training ProgramT32GM139791 · STANFORD UNIVERSITY · 2025 to 2025
$534k
Label-free Optical Recording of Neuroelectric ActivitiesR01NS121934 · STANFORD UNIVERSITY · 2025 to 2025
$430k
Homeostatic Regulation of PIP2-Calcium SignalingR01GM144479 · UT SOUTHWESTERN MEDICAL CENTER · 2025 to 2025
$388k
NHLBI NIH HHS R01 HL113006NHLBI NIH HHS R01 HL145676NHLBI NIH HHS R01 HL150693NHLBI NIH HHS R01 HL165491NIGMS NIH HHS R01 GM144479NIGMS NIH HHS R35 GM141598NIGMS NIH HHS T32 GM139791NINDS NIH HHS R01 NS121934U.S. Department of Health & Human Services | National Institutes of Health (NIH) 1R01NS121934U.S. Department of Health & Human Services | National Institutes of Health (NIH) R01HL165491
6 · The paper itself

Abstract

Contact sites between the endoplasmic reticulum (ER) and plasma membrane (PM) play a crucial role in governing calcium regulation and lipid homeostasis. Despite their significance, the factors regulating their spatial distribution on the PM remain elusive. Inspired by observations in cardiomyocytes, where ER-PM contact sites concentrate on tubular PM invaginations known as transverse tubules, we hypothesize that PM curvature plays a role in ER-PM contact formation. Through precise control of PM invaginations, we show that PM curvatures locally induce the formation of ER-PM contacts in cardiomyocytes. Intriguingly, the junctophilin family of ER-PM tethering proteins, specifically expressed in excitable cells, is the key player in this process, whereas the ubiquitously expressed extended synaptotagmin-2 does not show a preference for PM curvature. At the mechanistic level, we find that the low-complexity region (LCR) and membrane occupation and recognition nexus (MORN) motifs of junctophilins can bind independently to the PM, but both the LCR and MORN motifs are required for targeting PM curvatures. By examining the junctophilin interactome, we identify a family of curvature-sensing proteins-Eps15 homology domain-containing proteins-that interact with the MORN_LCR motifs and facilitate the preferential tethering of junctophilins to curved PM. These findings highlight the pivotal role of PM curvature in the formation of ER-PM contacts in cardiomyocytes and unveil a mechanism for the spatial regulation of ER-PM contacts through PM curvature modulation.

Indexed as

Cell MembraneEndoplasmic ReticulumMembrane ProteinsMyocytes, CardiacAnimalsHEK293 CellsHumansMiceProtein BindingRatsjunctophilinMembrane Proteins

Identifiers

PMID39289582
PMCPMC11567891

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.