Evidence mapPaperPMID 39294577Full record

SynthesisBMC infectious diseases2024

Exploring the clinical and diagnostic value of metagenomic next-generation sequencing for urinary tract infection: a systematic review and meta-analysis.

Sike He, Haolin Liu, Xu Hu, Jinge Zhao, Jiayu Liang, Xingming Zhang, Junru Chen, Hao Zeng, Guangxi Sun

Abstract readSystematic ReviewMeta-Analysis
In one paragraph

Synthesis in BMC infectious diseases, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed, 3 pooled it
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 3 syntheses or guidelines pooled it.

  1. Pooled it
  2. Pooled it
  3. Diagnostic performance of FEVChronic respiratory disease
    Pooled it
  4. Clinical utility of metagenomic next-generation sequencing in diagnosing spinal infections: a systematic review and meta-analysis.European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society · 2026
    Review
  5. Observational
  6. Article
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Sike He *Department of Urology, Institute of Urology, West China Hospital, Sichuan University, Chengdu, China.
Haolin Liu *Department of Urology, Institute of Urology, West China Hospital, Sichuan University, Chengdu, China.
Xu HuDepartment of Urology, Institute of Urology, West China Hospital, Sichuan University, Chengdu, China.
Jinge ZhaoDepartment of Urology, Institute of Urology, West China Hospital, Sichuan University, Chengdu, China.
Jiayu LiangDepartment of Urology, Institute of Urology, West China Hospital, Sichuan University, Chengdu, China.
Xingming ZhangDepartment of Urology, Institute of Urology, West China Hospital, Sichuan University, Chengdu, China.
Junru ChenDepartment of Urology, Institute of Urology, West China Hospital, Sichuan University, Chengdu, China.
Hao ZengDepartment of Urology, Institute of Urology, West China Hospital, Sichuan University, Chengdu, China. kucaizeng@163.com.
Guangxi SunDepartment of Urology, Institute of Urology, West China Hospital, Sichuan University, Chengdu, China. sungx077@126.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundA new pathogen detection tool, metagenomic next-generation sequencing (mNGS), has been widely used for infection diagnosis, but the clinical and diagnostic value of mNGS in urinary tract infection (UTI) remains inconclusive. This systematic review with meta-analysis aimed to investigate the efficacy of mNGS in treating UTIs.

methodsA comprehensive literature search was performed in PubMed, Web of Science, Embase, and the Cochrane Library, and eligible studies were selected based on the predetermined criteria. The quality of the included studies was assessed via the Quality Assessment of Diagnostic Accuracy Studies-2 (QUADAS-2) tool, and the certainty of evidence (CoE) was measured by the Grading of Recommendations, Assessment, Development, and Evaluations (GRADE) score. Then, the positive detection rate (PDR), pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), and area under the curve of the summary receiver operating characteristic curve (AUROC) was estimated in Review Manager, Stata, and MetaDisc. Subgroup analysis, meta-regression, and sensitivity analysis were performed to reveal the potential factors that influence internal heterogeneity.

resultsA total of 17 studies were selected for further analysis. The PDR of mNGS was markedly greater than that of culture (odds ratio (OR) = 2.87, 95% confidence interval [CI]: 1.72-4.81, p < 0.001, I

conclusionThe current evidence shows that mNGS has favorable diagnostic performance for UTIs. More high-quality prospective randomized controlled trials (RCTs) are expected to verify these findings and provide more information about mNGS in UTI treatment and prognosis.

Indexed as

High-Throughput Nucleotide SequencingMetagenomicsUrinary Tract InfectionsHumansROC CurveSensitivity and SpecificityDiagnostic performanceMeta-analysisMetagenomic next-generation sequencingUrinary tract infection

Identifiers

PMID39294577
PMCPMC11412013

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.