Evidence map›Paper›PMID 39298320›Full record

ArticleSTAR protocols2024

Protocol for quantum dot-based cell counting and immunostaining of pulmonary arterial cells from patients with pulmonary arterial hypertension.

Sakib M Moinuddin, Md Ibrahim, Tanoy Sarkar, Md Shahadat Hossain, Melanie Rose, Fakhrul Ahsan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Sakib M MoinuddinDepartment of Pharmaceutical and Biomedical Sciences, College of Pharmacy, California Northstate University, Elk Grove, CA 95757, USA.
Md IbrahimDepartment of Pharmaceutical and Biomedical Sciences, College of Pharmacy, California Northstate University, Elk Grove, CA 95757, USA. Electronic address: mohammad.ibrahim@cnsu.edu.
Tanoy SarkarDepartment of Pharmaceutical and Biomedical Sciences, College of Pharmacy, California Northstate University, Elk Grove, CA 95757, USA.
Md Shahadat HossainDepartment of Pharmaceutical and Biomedical Sciences, College of Pharmacy, California Northstate University, Elk Grove, CA 95757, USA.
Melanie RoseDepartment of Pharmaceutical and Biomedical Sciences, College of Pharmacy, California Northstate University, Elk Grove, CA 95757, USA.
Fakhrul AhsanDepartment of Pharmaceutical and Biomedical Sciences, College of Pharmacy, California Northstate University, Elk Grove, CA 95757, USA; Veterans Affairs Northern California Health Care System, Martinez, CA, USA. Electronic address: fakhrul.ahsan@cnsu.edu.

Funding

Inhaled Fasudil and DETA NONOate CAR-Targeted Liposomes for PAHR42HL151045 · NHLBI · VASCULAR BIOSCIENCES · PI AHSAN, FAKHRUL · 2020 to 2022
$2.6M
Recapitulation of sex- disparity in PAH on a microfluidic device and elucidation of the differences and similarities in the development, progression and therapy of PAH in male versus female patientsR01HL144590 · NHLBI · TEXAS TECH UNIVERSITY HEALTH SCIS CENTER · PI AHSAN, FAKHRUL · 2019 to 2022
$1.7M
PAH-on-a-chip: a novel disease-on-a-device model for studying the pathobiology of and screening drugs for pulmonary arterial hypertensionR43HL169134 · NHLBI · MEDLUIDICS LLC · PI AHSAN, FAKHRUL · 2023 to 2024
$427k
NHLBI NIH HHS R01 HL144590NHLBI NIH HHS R43 HL169134
6 · The paper itself

Abstract

Currently, there is no protocol for growing and culturing primary pulmonary arterial cells (PACs) available from the Pulmonary Hypertension Breakthrough Initiative (PHBI). Here, we present a protocol for cultivating and maintaining three major PACs collected from patients with pulmonary arterial hypertension (PAH): endothelial (PAH-ECs), smooth muscle (PAH-SMCs), and adventitial cells (PAH-ADCs). We describe steps for obtaining PACs from PHBI, evaluating the growth of cells labeled with quantum dots (QDs), and staining endothelial cell (EC) markers for immunofluorescence imaging. For complete details on the use and execution of this protocol, please refer to Al-Hilal et al.

Indexed as

Pulmonary ArteryQuantum DotsCell CountCells, CulturedEndothelial CellsHumansHypertension, PulmonaryMyocytes, Smooth MusclePulmonary Arterial HypertensionCell cultureCell isolationMicroscopyMolecular/Chemical Probes

Identifiers

PMID39298320
PMCPMC11426121

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.