Evidence map›Paper›PMID 39304868›Full record

ArticleBMC complementary medicine and therapies2024

Network pharmacology, molecular docking, and in vitro study on Aspilia pluriseta against prostate cancer.

Innocent Oluwaseun Okpako, Florence Atieno Ng'ong'a, Cleophas Mutinda Kyama, Sospeter Ngoci Njeru

Abstract read
In one paragraph

Article in BMC complementary medicine and therapies, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Innocent Oluwaseun OkpakoDepartment of Molecular Biology and Biotechnology, Pan African University Institute for Basic Sciences, Technology and Innovation, Nairobi, Kenya. innocentokpako9@gmail.com.
Florence Atieno Ng'ong'aDepartment of Biochemistry, School of Biomedical Sciences, College of Health Sciences, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya.
Cleophas Mutinda KyamaDepartment of Medical Laboratory Sciences, School of Biomedical Sciences, College of Health Sciences, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya.
Sospeter Ngoci NjeruCentre for Traditional Medicine and Drug Research and Centre for Community Driven Research, Kenya Medical Research Institute, Nairobi, Kenya. snjeru@kemri.go.ke.

Funding

Kenya Medical Research Institute REF: KEMRI/IRG/EC0017Pan African University Institute for Basic Sciences, Technology, and Innovation, Kenya REF: PAU/ADM/PAUSTI/2020/8
6 · The paper itself

Abstract

backgroundCurrent prostate cancer treatments are associated with life-threatening side effects, prompting the search for effective and safer alternatives. Aspilia pluriseta Schweinf. ex Engl. has previously shown anticancer activity in lung and liver cancer cell lines. This study investigated its potential for prostate cancer.

methodsA crude extract of A. pluriseta root was prepared using dichloromethane/methanol (1:1 v/v) and partitioned into hexane, ethyl acetate, and water fractions. The MTT assay was used to assess the antiproliferative activity of the fractions. The active fractions were tested at 6.25-200 µg/ml on human prostate cancer DU-145 cells and non-cancerous Vero E6 cells. Qualitative phytochemical and gas chromatography-mass spectrometry (GC-MS) analyses were conducted to identify chemical compounds. Network pharmacology was employed to predict molecular targets and modes of action of the identified chemical compounds, with subsequent validation through molecular docking and real-time PCR.

resultsActive extracts included crude dichloromethane/methanol, hexane, and ethyl acetate fractions, inhibiting DU-145 cell proliferation with IC

conclusionsA. pluriseta extracts inhibited DU-145 cell growth without causing cellular toxicity, suggesting great potential for development as an anti-prostate cancer agent. However, further in vitro and in vivo experiments are recommended.

Indexed as

Antineoplastic Agents, PhytogenicMolecular Docking SimulationNetwork PharmacologyPlant ExtractsProstatic NeoplasmsAnimalsCell Line, TumorCell ProliferationHumansMaleAntineoplastic Agents, PhytogenicPlant ExtractsAntiproliferative activityAspilia plurisetaGene expressionMolecular dockingNetwork pharmacologyProstate cancer

Identifiers

PMID39304868
PMCPMC11416023

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.