Evidence map›Paper›PMID 39305894›Full record

ArticleNeuron2024

A fast and responsive voltage indicator with enhanced sensitivity for unitary synaptic events.

Yukun A Hao, Sungmoo Lee, Richard H Roth, Silvia Natale, Laura Gomez, Jiannis Taxidis, Philipp S O'Neill, Vincent Villette, Jonathan Bradley, Zeguan Wang and 14 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuron, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.

0numbers the graph read from it
0cells of the map it votes in
40citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

40 citing papers in PubMed.

  1. Article
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  11. Causal and directional elements of global brain dynamics.bioRxiv : the preprint server for biology · 2026
    Article
  12. Article
  13. Article
  14. Cerebral capillary computation.American journal of physiology. Cell physiology · 2026
    Review
  15. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

24 authors.

Yukun A HaoDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Neurobiology, Stanford University, Stanford, CA 94305, USA.
Sungmoo LeeDepartment of Neurobiology, Stanford University, Stanford, CA 94305, USA.
Richard H RothDepartment of Neurosurgery, Stanford University, Stanford, CA 94305, USA.
Silvia NataleDepartment of Molecular & Cellular Physiology, Stanford University, Stanford, CA 94305, USA.
Laura GomezDepartment of Molecular and Cell Biology and Helen Wills Neuroscience Institute, University of California Berkeley, Berkeley, CA 94720, USA; Department of Physics, University of California Berkeley, CA 94720, USA.
Jiannis TaxidisDepartment of Neurology, UCLA David Geffen School of Medicine, Los Angeles, CA 90095, USA.
Philipp S O'NeillDepartment of Molecular Life Sciences, University of Zurich (UZH), 8057 Zurich, Switzerland; Neuroscience Center Zurich, 8057 Zurich, Switzerland.
Vincent VilletteInstitut de Biologie de l'École Normale Supérieure (IBENS), CNRS, INSERM, PSL Research University, Paris 75005, France.
Jonathan BradleyInstitut de Biologie de l'École Normale Supérieure (IBENS), CNRS, INSERM, PSL Research University, Paris 75005, France.
Zeguan WangDepartments of Brain and Cognitive Sciences, Media Arts and Sciences, and Biological Engineering, MIT, Cambridge, MA 02139, USA; McGovern Institute, MIT, Cambridge, MA 02139, USA.
Dongyun JiangDepartment of Neurobiology, Stanford University, Stanford, CA 94305, USA.
Guofeng ZhangDepartment of Neurobiology, Stanford University, Stanford, CA 94305, USA.
Mengjun ShengDepartment of Neurosurgery, Stanford University, Stanford, CA 94305, USA.
Di LuDepartment of Neurosurgery, Stanford University, Stanford, CA 94305, USA.
Edward BoydenDepartments of Brain and Cognitive Sciences, Media Arts and Sciences, and Biological Engineering, MIT, Cambridge, MA 02139, USA; McGovern Institute, MIT, Cambridge, MA 02139, USA; Howard Hughes Medical Institute, Cambridge, MA 02139, USA.
Igor DelvendahlDepartment of Molecular Life Sciences, University of Zurich (UZH), 8057 Zurich, Switzerland; Neuroscience Center Zurich, 8057 Zurich, Switzerland.
Peyman GolshaniDepartment of Neurology, UCLA David Geffen School of Medicine, Los Angeles, CA 90095, USA; Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine, Los Angeles, CA 90095, USA.
Marius WernigDepartment of Pathology, Stanford University, Stanford, CA 94305, USA.
Daniel E FeldmanDepartment of Molecular and Cell Biology and Helen Wills Neuroscience Institute, University of California Berkeley, Berkeley, CA 94720, USA.
Na JiDepartment of Molecular and Cell Biology and Helen Wills Neuroscience Institute, University of California Berkeley, Berkeley, CA 94720, USA; Department of Physics, University of California Berkeley, CA 94720, USA.
Jun DingDepartment of Neurosurgery, Stanford University, Stanford, CA 94305, USA.
Thomas C SüdhofDepartment of Molecular & Cellular Physiology, Stanford University, Stanford, CA 94305, USA; Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Thomas R ClandininDepartment of Neurobiology, Stanford University, Stanford, CA 94305, USA.
Michael Z LinDepartment of Bioengineering, Stanford University, Stanford, CA 94305, USA; Department of Neurobiology, Stanford University, Stanford, CA 94305, USA. Electronic address: mzlin@stanford.edu.

Funding

Validating Theoretical Models with Neurophysiology and OptogeneticsU19NS107613 · NINDS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI MILLER, KENNETH D · 2018 to 2022
$16.7M
Induced neuronal cells: A novel tool to study neuropsychiatric diseasesR01MH092931 · NIMH · STANFORD UNIVERSITY · PI Thomas C. Sudhof, Marius Wernig · 2010 to 2026
$9.7M
Novel Platforms for Systematic Optical Control of Complex Neural Circuits In VivoR01DA029639 · NIDA · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S. · 2010 to 2023
$6.7M
The power of positivity: a novel class of voltage indicators for high-fidelity brain activity imagingR01NS123681 · NINDS · STANFORD UNIVERSITY · PI LIN, MICHAEL Z. · 2021 to 2021
$3.6M
Dissecting Neural Circuit Computations in the Peripheral Visual SystemR01EY022638 · NEI · STANFORD UNIVERSITY · PI CLANDININ, THOMAS ROBERT · 2012 to 2021
$3.5M
Multidimensional Optimization of Voltage Indicators for In Vivo Neural Activity ImagingR01MH122971 · NIMH · BOSTON UNIVERSITY (CHARLES RIVER CAMPUS) · PI HAN, XUE · 2020 to 2024
$3.4M
High throughput assaying of circuit activity and connectivity in brain organoidsRF1MH123977 · NIMH · HARVARD UNIVERSITY · PI ARLOTTA, PAOLA, BOYDEN, EDWARD S. · 2020 to 2020
$2.5M
kHz-rate in vivo imaging of neural activity througout the living brainUF1NS107696 · NINDS · UNIVERSITY OF CALIFORNIA BERKELEY · PI JI, NA, TSIA, KEVIN KIN MAN · 2018 to 2018
$2.1M
Organization of neural coding and plasticity in L2/3 of mouse S1 cortexR01NS092367 · NINDS · UNIVERSITY OF CALIFORNIA BERKELEY · PI Daniel Feldman · 2023 to 2026
$1.9M
Adaptive optical microscopy for high-accuracy recording of neural activity in vivoU01NS118300 · NINDS · UNIVERSITY OF CALIFORNIA BERKELEY · PI JI, NA · 2021 to 2023
$1.7M
Multiplexing working memory and timing: Encoding retrospective and prospective information in transient neural trajectories.R01NS116589 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BUONOMANO, DEAN V, GOLSHANI, PEYMAN · 2023 to 2024
$1.2M
Population voltage imaging of sensory and cognitive signals in somatosensory (S1) cortexR21NS138989 · NINDS · UNIVERSITY OF CALIFORNIA BERKELEY · PI FELDMAN, DANIEL, JI, NA · 2024 to 2024
$399k
NEI NIH HHS R01 EY022638NIDA NIH HHS R01 DA029639NIMH NIH HHS R01 MH092931NIMH NIH HHS R01 MH122971NIMH NIH HHS RF1 MH123977NINDS NIH HHS R01 NS092367NINDS NIH HHS R01 NS116589NINDS NIH HHS R01 NS123681NINDS NIH HHS R21 NS138989NINDS NIH HHS U01 NS118300NINDS NIH HHS U19 NS107613NINDS NIH HHS UF1 NS107696
6 · The paper itself

Abstract

A remaining challenge for genetically encoded voltage indicators (GEVIs) is the reliable detection of excitatory postsynaptic potentials (EPSPs). Here, we developed ASAP5 as a GEVI with enhanced activation kinetics and responsivity near resting membrane potentials for improved detection of both spiking and subthreshold activity. ASAP5 reported action potentials (APs) in vivo with higher signal-to-noise ratios than previous GEVIs and successfully detected graded and subthreshold responses to sensory stimuli in single two-photon trials. In cultured rat or human neurons, somatic ASAP5 reported synaptic events propagating centripetally and could detect ∼1-mV EPSPs. By imaging spontaneous EPSPs throughout dendrites, we found that EPSP amplitudes decay exponentially during propagation and that amplitude at the initiation site generally increases with distance from the soma. These results extend the applications of voltage imaging to the quantal response domain, including in human neurons, opening up the possibility of high-throughput, high-content characterization of neuronal dysfunction in disease.

Indexed as

Action PotentialsExcitatory Postsynaptic PotentialsAnimalsCells, CulturedHEK293 CellsHumansNeuronsRatsSynapsesaction potentialsEPSPGEVIsubthreshold activitiesvoltage imaging

Identifiers

PMID39305894
PMCPMC11581914

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.