Evidence map›Paper›PMID 39306493›Full record

ArticleTrends in biotechnology2024

Engineering large-scale hiPSC-derived vessel-integrated muscle-like lattices for enhanced volumetric muscle regeneration.

Myung Chul Lee, Yasamin A Jodat, Yori Endo, Alejandra Rodríguez-delaRosa, Ting Zhang, Mehran Karvar, Ziad Al Tanoury, Jacob Quint, Tom Kamperman, Kiavash Kiaee and 25 more

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Article in Trends in biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

35 authors.

Myung Chul LeeDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA; Medicinal Materials Research Center, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea.
Yasamin A JodatDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA.
Yori EndoDepartment of Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Alejandra Rodríguez-delaRosaDepartment of Pathology, Brigham and Women's Hospital, Boston, MA 02115, USA; Department of Genetics, Harvard Medical School, Boston, MA 02115, USA; Harvard Stem Cell Institute, Harvard University, Boston, MA 02138, USA.
Ting ZhangDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA; Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China.
Mehran KarvarDepartment of Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Ziad Al TanouryDepartment of Pathology, Brigham and Women's Hospital, Boston, MA 02115, USA; Department of Genetics, Harvard Medical School, Boston, MA 02115, USA; Harvard Stem Cell Institute, Harvard University, Boston, MA 02138, USA.
Jacob QuintDepartment of Biomedical Engineering, University of Connecticut Health Center, Farmington, CT 06030, USA; Department of Mechanical and Materials Engineering, University of Nebraska, Lincoln, Lincoln, NE 68588, USA.
Tom KampermanDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA.
Kiavash KiaeeDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA.
Sofia Lara OchoaDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA.
Kun ShiDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA.
Yike HuangDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA.
Montserrat Pineda RosalesDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA.
Adnan ArnaoutDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA.
Hyeseon LeeDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA.
Jiseong KimDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA.
Eder Luna CeronDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA.
Isaac Garcia ReyesDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA.
Adriana C PanayiDepartment of Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Angel Flores Huidobro MartinezDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA.
Xichi WangDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA.
Ki-Tae KimDepartment of Molecular Genetics, School of Dentistry and Dental Research Institute, Dental Multi-omics Center, Seoul National University, Seoul 08826, Republic of Korea; Epigenetic Regulation of Aged Skeleto-Muscular System Laboratory, School of Dentistry and Dental Research Institute, Seoul National University, Seoul 08826, Republic of Korea.
Jae-I MoonDepartment of Molecular Genetics, School of Dentistry and Dental Research Institute, Dental Multi-omics Center, Seoul National University, Seoul 08826, Republic of Korea; Epigenetic Regulation of Aged Skeleto-Muscular System Laboratory, School of Dentistry and Dental Research Institute, Seoul National University, Seoul 08826, Republic of Korea.
Seung Gwa ParkDepartment of Molecular Genetics, School of Dentistry and Dental Research Institute, Dental Multi-omics Center, Seoul National University, Seoul 08826, Republic of Korea; Epigenetic Regulation of Aged Skeleto-Muscular System Laboratory, School of Dentistry and Dental Research Institute, Seoul National University, Seoul 08826, Republic of Korea.
Kangju LeeDepartment of Healthcare and Medical Engineering, Chonnam National University, Yeosu 59626, Republic of Korea.
Michelle A CalabreseChemical Engineering and Materials Science Department, University of Minnesota, Minneapolis, MN 55455, USA.
Shabir HassanDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA; Department of Biological Sciences, Khalifa University, Abu Dhabi 127788, United Arab Emirates; Biotechnology Center (BTC), Khalifa University, Abu Dhabi 127788, United Arab Emirates.
Junmin LeeDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA; Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea.
Ali TamayolDepartment of Biomedical Engineering, University of Connecticut Health Center, Farmington, CT 06030, USA; Department of Mechanical and Materials Engineering, University of Nebraska, Lincoln, Lincoln, NE 68588, USA.
Luke LeeDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA; Department of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Electrical Engineering and Computer Science, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Biophysics, Institute of Quantum Biophysics, Sungkyunkwan University, Suwon, Republic of Korea; Department of Chemistry and Nanoscience, Ewha Womans University, Seoul, Republic of Korea.
Olivier PourquiéDepartment of Pathology, Brigham and Women's Hospital, Boston, MA 02115, USA; Department of Genetics, Harvard Medical School, Boston, MA 02115, USA; Harvard Stem Cell Institute, Harvard University, Boston, MA 02138, USA.
Woo-Jin KimDepartment of Molecular Genetics, School of Dentistry and Dental Research Institute, Dental Multi-omics Center, Seoul National University, Seoul 08826, Republic of Korea; Epigenetic Regulation of Aged Skeleto-Muscular System Laboratory, School of Dentistry and Dental Research Institute, Seoul National University, Seoul 08826, Republic of Korea.
Indranil SinhaDepartment of Surgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Su Ryon ShinDivision of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA. Electronic address: sshin4@bwh.harvard.edu.

Funding

Suturable bioprinted vascularized muscle constructs for treatment of skeletal muscle lossR01AR077132 · NIAMS · BRIGHAM AND WOMEN'S HOSPITAL · PI SHIN, SU RYON, TAMAYOL, ALI · 2021 to 2025
$2.6M
Oxygen generating bioinks for 3D printed bone implantsR01AR074234 · NIAMS · BRIGHAM AND WOMEN'S HOSPITAL · PI SHIN, SU RYON · 2018 to 2022
$1.9M
Microengineered scaffolds carrying patient-specific cells and growth factors for treatment of volumetric muscle lossR01AR073822 · NIAMS · UNIVERSITY OF NEBRASKA LINCOLN · PI TAMAYOL, ALI · 2018 to 2022
$1.8M
Programmable multimaterial bioprinting of 3D vascularized tissue constructsR21EB026824 · NIBIB · BRIGHAM AND WOMEN'S HOSPITAL · PI SHIN, SU RYON · 2018 to 2020
$685k
NIAMS NIH HHS R01 AR073822NIAMS NIH HHS R01 AR074234NIAMS NIH HHS R01 AR077132NIBIB NIH HHS R21 EB026824
6 · The paper itself

Abstract

Engineering biomimetic tissue implants with human induced pluripotent stem cells (hiPSCs) holds promise for repairing volumetric tissue loss. However, these implants face challenges in regenerative capability, survival, and geometric scalability at large-scale injury sites. Here, we present scalable vessel-integrated muscle-like lattices (VMLs), containing dense and aligned hiPSC-derived myofibers alongside passively perfusable vessel-like microchannels inside an endomysium-like supporting matrix using an embedded multimaterial bioprinting technology. The contractile and millimeter-long myofibers are created in mechanically tailored and nanofibrous extracellular matrix-based hydrogels. Incorporating vessel-like lattice enhances myofiber maturation in vitro and guides host vessel invasion in vivo, improving implant integration. Consequently, we demonstrate successful de novo muscle formation and muscle function restoration through a combinatorial effect between improved graft-host integration and its increased release of paracrine factors within volumetric muscle loss injury models. The proposed modular bioprinting technology enables scaling up to centimeter-sized prevascularized hiPSC-derived muscle tissues with custom geometries for next-generation muscle regenerative therapies.

Indexed as

Induced Pluripotent Stem CellsRegenerationTissue EngineeringAnimalsBioprintingHumansHydrogelsMiceMuscle, SkeletalTissue ScaffoldsHydrogelsbioprintingengineering vascularized tissuesinduced pluripotent stem cellssecreted factors, volumetric muscle lossskeletal muscle engineering

Identifiers

PMID39306493
PMCPMC11625013

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.