ArticleThe New phytologist2025
CRISPR/Cas9-based editing of NF-YC4 promoters yields high-protein rice and soybean.
Article in The New phytologist, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Ghd7‑triggered transcriptional regulation for synergistically improving rice eating quality and yield.Nature communications · 2026Article
- Genome-Wide Characterization of Nuclear Factor Y (NF-Y) Transcription Factors in Allohexaploid Oat.Plants (Basel, Switzerland) · 2026Article
- Natural Variation and Expression of OsCFF1 Affect the Formation of Rice Chalkiness.Rice (New York, N.Y.) · 2026Article
- Molecular regulators of regeneration and strategies for overcoming genotype-dependent recalcitrance in wheat (Triticum aestivum).Communications biology · 2026Review
- AtMYB72 as a Biotechnological Tool to Overcome Phenylpropanoid Substrate Limitation and Enhance Coumarin Biosynthesis in Plants.Plant biotechnology journal · 2026Article
- Breeding of a Multifoliolate Alfalfa Population Using CRISPR/Cas9-Generated Mutants and Evaluation of Agronomic Traits and Nutritive Value.Plants (Basel, Switzerland) · 2026Article
- Genetic Determinants and Breeding Strategies for Rice Cooking and Eating Quality: A Comprehensive Review.Plant biotechnology journal · 2026Review
- Precision breeding in a changing climate: unlocking resilience through omics and gene editing.Functional & integrative genomics · 2026Review
- CRISPR/Cas9 in soybean research: a measured leap toward genetic refinement.Frontiers in genome editing · 2026Review
- Metabolic engineering of soybean for improving grain quality for animal consumption.Frontiers in plant science · 2026Review
- Engineering non-coding DNA Elements in Rice: an Elegant Approach To fine-tune Agronomical Advantageous Traits.Rice (New York, N.Y.) · 2025Review
- Integrating Drought Stress Signaling and Smart Breeding for Climate-Resilient Crops: Regulatory Mechanisms and Genetic Strategies.Plants (Basel, Switzerland) · 2025Review
- Nuclear factor-Y transcription factors in crops: Biological roles, regulation, and breeding applications.Plant communications · 2025Review
- Exonuclease-fused CRISPR-cas system enhances targeted genome editing for functional genomics in soybean.BMC plant biology · 2025Article
- Promoter editing of LpNOL generates stay-green perennial ryegrass with improved forage quality and heat tolerance.Plant physiology · 2025Article
- Editing cis-elements of OsPHO1;2 improved phosphate transport and yield in rice.Plant biotechnology journal · 2025Article
- CRISPR/Cas-Mediated Optimization of Soybean Shoot Architecture for Enhanced Yield.International journal of molecular sciences · 2025Review
- Conserved language of plant gene regulation.Plant physiology · 2025Article
- A panomics-driven framework for the improvement of major food legume crops: advances, challenges, and future prospects.Horticulture research · 2025Article
- Thermotolerance for yield and quality in rice: a novel NF-Ys-QT12 gene system.Functional & integrative genomics · 2025Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Genome editing is a revolution in biotechnology for crop improvement with the final product lacking transgenes. However, most derived traits have been generated through edits that create gene knockouts. Our study pioneers a novel approach, utilizing gene editing to enhance gene expression by eliminating transcriptional repressor binding motifs. Building upon our prior research demonstrating the protein-boosting effects of the transcription factor NF-YC4, we identified conserved motifs targeted by RAV and WRKY repressors in the NF-YC4 promoters from rice (Oryza sativa) and soybean (Glycine max). Leveraging CRISPR/Cas9 technology, we deleted these motifs, resulting in reduced repressor binding and increased NF-YC4 expression. This strategy led to increased protein content and reduced carbohydrate levels in the edited rice and soybean plants, with rice exhibiting up to a 68% increase in leaf protein and a 17% increase in seed protein, and soybean showing up to a 25% increase in leaf protein and an 11% increase in seed protein. Our findings provide a blueprint for enhancing gene expression through precise genomic deletions in noncoding sequences, promising improved agricultural productivity and nutritional quality.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.