Evidence map›Paper›PMID 39317913›Full record

ArticleJournal of assisted reproduction and genetics2024

TET1 overexpression affects cell proliferation and apoptosis in aging ovaries.

Qiang Feng, Qirong Li, Yurui Hu, Zhan Wang, Hengzong Zhou, Chao Lin, Dongxu Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Qiang Feng *Laboratory Animal Center, College of Animal Science, Jilin University, Changchun, 130062, China.
Qirong Li *Laboratory Animal Center, College of Animal Science, Jilin University, Changchun, 130062, China.
Yurui HuLaboratory Animal Center, College of Animal Science, Jilin University, Changchun, 130062, China.
Zhan WangLaboratory Animal Center, College of Animal Science, Jilin University, Changchun, 130062, China.
Hengzong ZhouLaboratory Animal Center, College of Animal Science, Jilin University, Changchun, 130062, China.
Chao LinSchool of Grain Science and Technology, Jilin Business and Technology College, Changchun, 130062, China.
Dongxu WangLaboratory Animal Center, College of Animal Science, Jilin University, Changchun, 130062, China. wang_dong_xu@jlu.edu.cn.ORCID http://orcid.org/0000-0003-0464-1596

Funding

Jilin Education Department Program JJKH20230259KJJilin Province Development and Reform Commission 2023C028-6Jilin Province University Key Laboratory Scientific Research Program [2019] No. 004Jilin Provincial Scientific and Technological Development Program 20220505033ZP
6 · The paper itself

Abstract

purposeAlong with the progress of society, human life expectancy has been increasing, and late marriage and late childbearing are the current trend. Since reproductive aging affects fertility, ovarian aging in women has become a major reproductive health issue in the current society. During ovarian aging, DNA methylation levels may change. The ten-eleven translocation (TET) protein family proteins TET1, TET2, and TET3 are important DNA demethylation enzymes, and differential expression of TET1, TET2, and TET3 may affect the proliferation and apoptosis of aging ovarian cells. The aim of this study was to investigate the role of TET1 in the regulation of ovarian aging.

methodsThe expression of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) was analyzed by immunofluorescence (IF) in young and aging ovaries of six 6-8-week-old female mice and six 6-8-month-old female mice. Then, the expression pattern of the TET protein family in young and aging ovaries of mice was investigated. To determine the impact of TET1 on ovarian development, the aging of IOSE-80, KGN, and SKOV-3 cells was induced with D-galactosidase (D-gal). Cells were then transfected using the TET1 overexpression vector or si-TET1. We assessed the proliferation and apoptosis of aging cells after transfection and analyzed the regulatory effect of TET1 expression on aging cells. Additionally, we verified the Tet1 expression in Tet1-KO mice.

resultsThe 5mC to 5hmC transition, oocyte maturation, and blastocyst rate were reduced in aging mice compared to young mice. In aging mice ovaries, the expression levels of Tet1, Tet2, and Tet3 were reduced significantly, with Tet1 being particularly pronounced. The overexpression of TET1 promoted proliferation and inhibited apoptosis in aging human ovarian cells. Furthermore, Tet1 expression was very low in Tet1-KO C57BL/6 J mice ovaries.

conclusionThis study demonstrates that the expression levels of TET family proteins are low in aging ovaries, and the overexpression of TET1 can promote proliferation and inhibit apoptosis in aging ovarian cells.

Indexed as

AgingApoptosisCell ProliferationDNA-Binding ProteinsDNA MethylationOvaryProto-Oncogene Proteins5-MethylcytosineAnimalsDioxygenasesFemaleHumansMiceMixed Function Oxygenases5-hydroxymethylcytosine5-MethylcytosineDioxygenasesDNA-Binding ProteinsMixed Function OxygenasesProto-Oncogene ProteinsTET1 protein, humanTET1 protein, mouse5hmCAgingOvarianTET1Young

Identifiers

PMID39317913
PMCPMC11707214

What Socratic holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.