Predicting leaf nitrogen content in wolfberry trees by hyperspectral transformation and machine learning for precision agriculture.

Leaf nitrogen content (LNC) is an important indicator for scientific diagnosis of the nutrition status of crops. It plays an important role in the growth, yield and quality of wolfberry. This study aimed to develop new spectral indices (NSIs) and constructed machine learning regression (MLR) models for predicting wolfberry tree LNC. By utilizing four smoothing methods and five mathematic transformation methods, we obtained the original spectral dataset and five spectral transformation datasets for quantitative analysis and model establishment. Subsequently, published vegetation indices (PVIs) were acquired, sensitive wavelengths (SWs) were screened and NSIs were calculated based on SWs. Then MLR models were constructed by combining NSIs from six spectral datasets with three machine learning algorithms. Finally, a comparison was made among the MLR models. The study indicated that the application of mathematical transformation highlighted the differences in spectra, the square root, first-derivative and second-derivative transformation improved the prediction accuracy of MLR models constructed based on NSIs (MLR-NSIs models). However, these transformations had little impact on improving the prediction ability of MLR models constructed based on PVIs (MLR-PVIs models). Additionally, The optimal model for predicting the LNC of wolfberry tree was obtained by using the Random Forest (RF) algorithm combined with NSIs developed by first-derivative transformation spectra. The determination coefficient of validation (Rv2) and ratio of percentage deviation (RPD) was 0.71 and 1.90, respectively. In conclusion, this study has demonstrated that the combination of hyperspectral transformation and machine learning is useful for improving the accuracy of LNC estimation in wolfberry trees.