Evidence mapPaperPMID 39329747Full record

ArticleCells2024

The Nuclear Speckles Protein SRRM2 Is Exposed on the Surface of Cancer Cells.

Markus Kellner, Julia Hörmann, Susanne Fackler, Yuanyu Hu, Tielin Zhou, Lin Lu, Ibrahim Ilik, Tugce Aktas, Regina Feederle, Stefanie M Hauck and 4 more

Abstract read
In one paragraph

Article in Cells, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Markus KellnerInstitute of Structural Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Feodor-Lynen-Str. 21, 81377 Munich, Germany.
Julia HörmannInstitute of Structural Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Feodor-Lynen-Str. 21, 81377 Munich, Germany.
Susanne FacklerInstitute of Structural Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Feodor-Lynen-Str. 21, 81377 Munich, Germany.
Yuanyu HuZeno Therapeutics Pte. Ltd., 600 North Bridge Road, Singapore 188778, Singapore.ORCID 0000-0001-5457-4331
Tielin ZhouZeno Therapeutics Pte. Ltd., 600 North Bridge Road, Singapore 188778, Singapore.
Lin LuZeno Therapeutics Pte. Ltd., 600 North Bridge Road, Singapore 188778, Singapore.
Ibrahim IlikOtto Warburg Laboratories, 14195 Berlin, Germany.
Tugce AktasOtto Warburg Laboratories, 14195 Berlin, Germany.
Regina FeederleCore Facility Monoclonal Antibodies, Helmholtz Zentrum München, German Research Center for Environmental Health, 85764 Neuherberg, Germany.ORCID 0000-0002-3981-367X
Stefanie M HauckProteomics and Metabolomics Core, Helmholtz Zentrum München, German Research Center for Environmental Health, 85764 Neuherberg, Germany.
Olivier GiresDepartment of Otorhinolaryngology, LMU University Hospital, 81377 Munich, Germany.ORCID 0000-0002-2292-7064
Kathrin GärtnerEximmium Biotechnologies GmbH, 81377 Munich, Germany.ORCID 0000-0001-5244-4859
Lietao LiZeno Therapeutics Pte. Ltd., 600 North Bridge Road, Singapore 188778, Singapore.
Reinhard ZeidlerInstitute of Structural Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Feodor-Lynen-Str. 21, 81377 Munich, Germany.ORCID 0000-0002-3357-4954

Funding

Helmholtz Zentrum München German Research Center for Environmental Health n.a.
6 · The paper itself

Abstract

The membrane composition of extracellular vesicles (EVs) largely reflects that of the plasma membrane of the cell of origin. We therefore hypothesized that EVs could be used for immunizations to generate monoclonal antibodies against well-known tumor antigens but possibly also against hitherto unknown tumor-associated target molecules. From an immunization experiment, we obtained a monoclonal antibody specific for SRRM2, an RNA-binding protein involved in splicing and a major component of nuclear speckles. Here, we used this antibody to demonstrate that SRRM2 is exposed on the surface of most cancer cell lines from various entities and, even more important, on cancer cells in vivo. Moreover, we demonstrated that SRRM2-specific CAR-T cells are functional in vitro and in vivo. Collectively, we identified SRRM2 as a promising new target molecule exposed on the cancer cell surface and showed that our SRRM2-specific antibody can be used as a basis for the development of new targeted cancer therapies.

Indexed as

NeoplasmsAnimalsAntibodies, MonoclonalCell Line, TumorCell MembraneExtracellular VesiclesHumansMiceRNA-Binding ProteinsAntibodies, MonoclonalRNA-Binding Proteinscancer targetSRRM2target identificationtherapeutic antibodies

Identifiers

PMID39329747
PMCPMC11430694

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.