Evidence map›Paper›PMID 39334355›Full record

ArticleLipids in health and disease2024

Longitudinal association of peripheral blood DNA methylation with liver fat content: distinguishing between predictors and biomarkers.

Hailuan Zeng, Wenran Li, Mingfeng Xia, Jieyu Ge, Hui Ma, Lingyan Chen, Baishen Pan, Huandong Lin, Sijia Wang, Xin Gao

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In one paragraph

Article in Lipids in health and disease, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Hailuan Zeng *Department of Endocrinology and Metabolism, Zhongshan Hospital, Fudan University, NO. 180 Fenglin Road, Shanghai, 200032, China.
Wenran Li *CAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China.
Mingfeng XiaDepartment of Endocrinology and Metabolism, Zhongshan Hospital, Fudan University, NO. 180 Fenglin Road, Shanghai, 200032, China.
Jieyu GeCAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China.
Hui MaDepartment of Geriatrics, Zhongshan Hospital, Fudan University, Shanghai, China.
Lingyan ChenDepartment of Geriatrics, Zhongshan Hospital, Fudan University, Shanghai, China.
Baishen PanDepartment of Laboratory Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.
Huandong LinDepartment of Endocrinology and Metabolism, Zhongshan Hospital, Fudan University, NO. 180 Fenglin Road, Shanghai, 200032, China. lin.huandong@zs-hospital.sh.cn.
Sijia WangCAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China. wangsijia@picb.ac.cn.
Xin GaoDepartment of Endocrinology and Metabolism, Zhongshan Hospital, Fudan University, NO. 180 Fenglin Road, Shanghai, 200032, China. zhongshan_endo@126.com.

Funding

Chinese Academy of Sciences Young Team Program for Stable Support of Basic Research YSBR-077Outstanding Resident Clinical Postdoctoral Program of Zhongshan Hospital Affiliated to Fudan University 2023ZYYS-003Shanghai Municipal Science and Technology Major Project 2017SHZDZX01the National Natural Science Foundation of China 32200472
6 · The paper itself

Abstract

backgroundAlterations in DNA methylation (DNAm) have been observed in patients with fatty liver, but whether they are cause or consequence remains unknown. The study aimed to investigate longitudinal association of epigenome-wide DNAm with liver fat content (LFC) in Chinese participants, and explore their temporal relationships.

methodsData were obtained from 2 waves over a four-year time period of the Shanghai Changfeng Study (discovery, n = 407 and replication, n = 126). LFC and peripheral blood DNAm were repeatedly measured using quantitative hepatic ultrasonography and the 850 K Illumina EPIC BeadChip, respectively. Longitudinal and cross-sectional epigenome-wide association studies (EWASs) were conducted with linear mixed model and linear regression model, respectively. Meta-analysis was performed using METAL. Cross-lagged panel analysis (CLPA) was carried out to infer temporal relationships between the significant CpGs and LFC.

resultsLongitudinal EWAS identified cg11024682 (SREBF1), cg06500161 (ABCG1), cg16740586 (ABCG1), cg15659943 (ABCA1) and cg00163198 (SNX19) significantly associated with LFC with P < 1e-7. Another 6 of the 22 previously reported CpGs were replicated in the present longitudinal EWAS. CLPA showed longitudinal effects of cg11024682 (SREBF1) (β = 0.14 [0.06, 0.23]), cg16740586 (ABCG1) (β = 0.17 [0.08, 0.25]), cg06500161 (ABCG1) (β = 0.12 [0.03, 0.20]), cg17901584 (DHCR24) (β = -0.10 [-0.18, -0.02]), cg00574958 (CPT1A) (β = -0.09 [-0.17, -0.01]), cg08309687 (LINC00649) (β = -0.11 [-0.19, -0.03]), and cg27243685 (ABCG1) (β = 0.09 [0.01, 0.18]) on subsequent LFC. The effects were attenuated when further adjusting for body mass index. High levels of LFC led to alterations in DNAm of cg15659943 (ABCA1) (β = 0.13 [0.04, 0.21]), cg07162647 (β = -0.11 [-0.19, -0.03]), cg06500161 (ABCG1) (β = 0.10 [0.02, 0.18]), and cg27243685 (ABCG1) (β = 0.10 [0.02, 0.18]).

conclusionsBlood DNAm at SREBF1, ABCG1, DHCR24, CPT1A, and LINC00649 may be predictors of subsequent LFC change. The effects of DNAm at SREBF1 and ABCG1 on LFC were partially influenced by obesity. The findings have potential implications in understanding disease pathogenesis and highlight the potential of DNAm for early detection or intervention of fatty liver.

Indexed as

ATP Binding Cassette Transporter, Subfamily G, Member 1BiomarkersDNA MethylationLiverAdultATP Binding Cassette Transporter 1CpG IslandsCross-Sectional StudiesEpigenesis, GeneticFemaleGenome-Wide Association StudyHumansLongitudinal StudiesMaleMiddle AgedSterol Regulatory Element Binding Protein 1ABCA1 protein, humanABCG1 protein, humanATP Binding Cassette Transporter 1ATP Binding Cassette Transporter, Subfamily G, Member 1BiomarkersSREBF1 protein, humanSterol Regulatory Element Binding Protein 1Cross-lagged panel analysisDNA methylationLiver fat contentLongitudinal study

Identifiers

PMID39334355
PMCPMC11429307

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.