Evidence map›Paper›PMID 39334413›Full record

ArticleHuman genomics2024

Forward-reverse mutation cycles in cancer cell lines under chemical treatments.

Si Chen, Iram S Tyagi, Wai Kin Mat, Muhammad A Khan, Weijian Fan, Zhenggang Wu, Taobo Hu, Can Yang, Hong Xue

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Human genomics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Si Chen *Guangzhou HKUST Fok Ying Tung Research Institute, Science and Technology Building, Nansha Information Technology Park, Nansha, Guangzhou, 511458, China.
Iram S Tyagi *Division of Life Science and Center of Applied Genomics, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China.
Wai Kin MatDivision of Life Science and Center of Applied Genomics, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China.
Muhammad A KhanDivision of Life Science and Center of Applied Genomics, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China.
Weijian FanDivision of Life Science and Center of Applied Genomics, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China.
Zhenggang WuGuangzhou HKUST Fok Ying Tung Research Institute, Science and Technology Building, Nansha Information Technology Park, Nansha, Guangzhou, 511458, China.
Taobo HuDivision of Life Science and Center of Applied Genomics, Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China.
Can YangGuangzhou HKUST Fok Ying Tung Research Institute, Science and Technology Building, Nansha Information Technology Park, Nansha, Guangzhou, 511458, China. macyang@ust.hk.
Hong XueGuangzhou HKUST Fok Ying Tung Research Institute, Science and Technology Building, Nansha Information Technology Park, Nansha, Guangzhou, 511458, China. hxue@ust.hk.

Funding

Basic and Applied Basic Research Foundation of Guangdong Province 2021A1515011169Innovation and Technology Commission ITS/113/15FPInnovation and Technology Commission ITT/023/17GPInnovation and Technology Commission ITT/026/18GPShenzhen Municipal Science and Technology Bureau SZ-SZST11808University Grants Committee DG17SC01University Grants Committee SRF111EG01University Grants Committee SRF111EG04PGUniversity Grants Committee VPRDO09/10.SC08
6 · The paper itself

Abstract

Spontaneous forward-reverse mutations were reported by us earlier in clinical samples from various types of cancers and in HeLa cells under normal culture conditions. To investigate the effects of chemical stimulations on such mutation cycles, the present study examined single nucleotide variations (SNVs) and copy number variations (CNVs) in HeLa and A549 cells exposed to wogonin-containing or acidic medium. In wogonin, both cell lines showed a mutation cycle during days 16-18. In acidic medium, both cell lines displayed multiple mutation cycles of different magnitudes. Genomic feature colocalization analysis suggests that CNVs tend to occur in expanded and unstable regions, and near promoters, histones, and non-coding transcription sites. Moreover, phenotypic variations in cell morphology occurred during the forward-reverse mutation cycles under both types of chemical treatments. In conclusion, chemical stresses imposed by wogonin or acidity promoted cyclic forward-reverse mutations in both HeLa and A549 cells to different extents.

Indexed as

DNA Copy Number VariationsFlavanonesMutationA549 CellsCell Line, TumorHeLa CellsHumansNeoplasmsPolymorphism, Single NucleotideFlavanoneswogoninAcidic culture mediumAluScanAnticancer agentCell culturesCell morphology changeCopy number variationGenome instabilityNext generation sequencingSingle nucleotide variationWogonin

Identifiers

PMID39334413
PMCPMC11437743

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.