Evidence map›Paper›PMID 39335388›Full record

ArticleBrain sciences2024

A Two-Hit Approach Inducing Flurothyl Seizures in Fmr1 Knockout Mice Impacts Anxiety and Repetitive Behaviors.

Katherine J Blandin, David A Narvaiz, Donald Gregory Sullens, Paige D Womble, Samantha L Hodges, Matthew S Binder, Amanda Faust, Phuoc H Nguyen, Zachary J Pranske, Joaquin N Lugo

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Article in Brain sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. International journal of molecular sciences · 2026
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Katherine J BlandinDepartment of Psychology and Neuroscience, Baylor University, Waco, TX 76798, USA.
David A NarvaizDepartment of Psychology and Neuroscience, Baylor University, Waco, TX 76798, USA.
Donald Gregory SullensDepartment of Psychology and Neuroscience, Baylor University, Waco, TX 76798, USA.ORCID 0000-0002-6426-2355
Paige D WombleDepartment of Psychology and Neuroscience, Baylor University, Waco, TX 76798, USA.ORCID 0000-0003-0999-1050
Samantha L HodgesDepartment of Pharmacology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
Matthew S BinderDepartment of Neurosurgery, Yale University School of Medicine, East Haven, CT 06520, USA.
Amanda FaustDepartment of Psychology and Neuroscience, Baylor University, Waco, TX 76798, USA.ORCID 0009-0003-1801-6801
Phuoc H NguyenDepartment of Neuroscience, University of Maryland, Baltimore, MD 20742, USA.
Zachary J PranskeDepartment of Biology, Brandeis University, Waltham, MA 02453, USA.ORCID 0000-0001-9364-622X
Joaquin N LugoDepartment of Psychology and Neuroscience, Baylor University, Waco, TX 76798, USA.ORCID 0000-0001-6874-8718

Funding

Signaling Mechanisms Underlying Epilepsy and Autism CormorbidityR15NS088776 · NINDS · BAYLOR UNIVERSITY · PI JOAQUIN N LUGO · 2015 to 2026
$1.8M
National Institutes of Health NINDS NS088776
6 · The paper itself

Abstract

backgroundFragile X Syndrome (FXS) is the leading monogenetic cause of autism spectrum disorder (ASD) and is associated with seizures. We examined the impact of repeated seizures on the behavioral and molecular changes in male Fmr1 knockout (KO) mice and wild-type (WT) mice.

methodsSeizures were induced by administering three flurothyl seizures per day across postnatal days (PD) 7-11, for a total of 15 seizures. In adulthood, mice were tested in a battery of behavioral tasks to assess long-term behavioral deficits.

resultsThe two-hit impact of a Fmr1 knockout and seizures resulted in decreased anxiety-like behavior in the elevated plus maze test and a longer latency to their first nose poke (repetitive behavior). Seizures resulted in decreased activity, decreased repetitive behavior (grooming and rearings), and decreased social behavior, while they also increased habituation to auditory stimuli and increased freezing in delayed fear conditioning in both KO and control mice. KO mice displayed increased repetitive behavior in the open field task (clockwise revolutions) and repeated nose pokes, and decreased anxiety in the open field test. No differences in mTOR signaling were found.

conclusionsThese findings further illuminate the long-term effects of synergistic impact of two hits on the developing brain.

Indexed as

developmental epilepsydouble-hitearly life seizuresepilepsymTORtwo-hit

Identifiers

PMID39335388
PMCPMC11429635

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.