Evidence map›Paper›PMID 39335593›Full record

ArticleBiomedicines2024

Comparison of Different Animal Models in Hindlimb Functional Recovery after Acute Limb Ischemia-Reperfusion Injury.

Nadezhda N Zheleznova, Claire Sun, Nakul Patel, Nathan Hall, Kristof M Williams, Jie Zhang, Jin Wei, Lusha Xiang, Ridham Patel, Sahil Soni and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedicines, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Nadezhda N ZheleznovaDepartment of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, FL 33612, USA.ORCID 0009-0001-3894-1936
Claire SunDepartment of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, FL 33612, USA.
Nakul PatelDepartment of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, FL 33612, USA.
Nathan HallDepartment of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, FL 33612, USA.
Kristof M WilliamsDepartment of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, FL 33612, USA.
Jie ZhangDivision of Nephrology at Boston Medical Center, Department of Medicine, Boston University Chobanian and Avedisian School of Medicine, Boston, MA 02118, USA.
Jin WeiDivision of Nephrology at Boston Medical Center, Department of Medicine, Boston University Chobanian and Avedisian School of Medicine, Boston, MA 02118, USA.
Lusha XiangUnited States Army Institute of Surgical Research, 3698 Chambers Pass BLDG 3611, Ft. Sam Houston, TX 78234, USA.ORCID 0000-0002-7255-1640
Ridham PatelDepartment of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, FL 33612, USA.
Sahil SoniDepartment of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, FL 33612, USA.
Divya ShethDepartment of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, FL 33612, USA.
Enyin LaiDepartment of Physiology, Zhejiang University School of Medicine, Hangzhou 310058, China.ORCID 0000-0003-3074-6869
Xingyu QiuDepartment of Physiology, Zhejiang University School of Medicine, Hangzhou 310058, China.
Nohely Hernandez SotoDepartment of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, FL 33612, USA.
Ruisheng LiuDepartment of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, FL 33612, USA.ORCID 0000-0002-2220-1888

Funding

Tubuloglomerular feedback response in AKI to CKD transitionR01DK134028 · NIDDK · UNIVERSITY OF SOUTH FLORIDA · PI LIU, RUISHENG · 2022 to 2025
$2.6M
Role of tubuloglomerular feedback in the development of hypertension in diabetesR01HL142814 · NHLBI · UNIVERSITY OF SOUTH FLORIDA · PI LIU, RUISHENG · 2019 to 2022
$2.4M
Protection of donor kidney and transplanted graft function by modulating Na/K ATPase activityR01DK138092 · NIDDK · UNIVERSITY OF SOUTH FLORIDA · PI WEI CHEN, RUISHENG LIU · 2024 to 2026
$2.0M
Treatment of lupus nephritis with nanoparticles that selectively target kidney glomeruliR01DK134000 · NIDDK · UNIVERSITY OF SOUTH FLORIDA · PI RUISHENG LIU, Shyam S Mohapatra · 2023 to 2026
$1.9M
NHLBI NIH HHS R01 HL142814NIDDK NIH HHS R01 DK134000NIDDK NIH HHS R01 DK134028NIDDK NIH HHS R01 DK138092NIH HHS HL142814, DK134000, DK134028, and DK138092
6 · The paper itself

Abstract

Acute limb ischemia (ALI) is a sudden lack of blood flow to a limb, primarily caused by arterial embolism and thrombosis. Various experimental animal models, including non-invasive and invasive methods, have been developed and successfully used to induce limb ischemia-reperfusion injuries (L-IRI). However, there is no consensus on the methodologies used in animal models for L-IRI, particularly regarding the assessment of functional recovery. The present study aims to compare different approaches that induce L-IRI and determine the optimal animal model to study functional limb recovery. In this study, we applied a pneumatic cuff as a non-invasive method and ligated the aorta, iliac, or femoral artery as invasive methods to induce L-IRI. We have measured grip strength, motor function, creatine kinase level, inflammatory markers such as nuclear factor NF-κB, interleukin-6 (IL-6), hypoxia markers such as hypoxia-induced factor-1α (HIF-1α), and evaluated the muscle injury with hematoxylin and eosin (H&E) staining in Sprague Dawley rats after inducing L-IRI. The pneumatic pressure cuff method significantly decreased the muscle strength of the rats, causing the loss of ability to hold the grid and inducing significant limb function impairment, while artery ligations did not. We conclude from this study that the tourniquet cuff method could be ideal for studying functional recovery after L-IRI in the rat model.

Indexed as

aortaarterycreatine kinasefemoralgrip strengthiliacligationlimb ischemiamotor functionmuscle injurypneumatic cuffSD rat

Identifiers

PMID39335593
PMCPMC11428748

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.