Evidence mapPaperPMID 39338354Full record

ArticlePharmaceuticals (Basel, Switzerland)2024

The Effect of Chronic Immunosuppressive Regimen Treatment on Apoptosis in the Heart of Rats.

Anna Surówka, Michał Żołnierczuk, Piotr Prowans, Marta Grabowska, Patrycja Kupnicka, Marta Markowska, Zbigniew Szlosser, Karolina Kędzierska-Kapuza

Abstract read
In one paragraph

Article in Pharmaceuticals (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Anna SurówkaDepartment of Plastic, Endocrine and General Surgery, Pomeranian Medical University, 72-010 Szczecin, Poland.ORCID 0000-0002-8693-9259
Michał ŻołnierczukDepartment of Vascular Surgery, General Surgery and Angiology, Pomeranian Medical University, 70-111 Szczecin, Poland.ORCID 0000-0002-4539-7342
Piotr ProwansDepartment of Plastic, Endocrine and General Surgery, Pomeranian Medical University, 72-010 Szczecin, Poland.
Marta GrabowskaDepartment of Histology and Developmental Biology, Faculty of Health Sciences, Pomeranian Medical University, 70-111 Szczecin, Poland.ORCID 0000-0003-0861-0738
Patrycja KupnickaDepartment of Biochemistry and Medical Chemistry, Pomeranian Medical University, 70-111 Szczecin, Poland.ORCID 0000-0002-0756-6483
Marta MarkowskaDepartment of Plastic and Reconstructive Surgery, 109 Military Hospital, 71-422 Szczecin, Poland.
Zbigniew SzlosserDepartment of Plastic, Endocrine and General Surgery, Pomeranian Medical University, 72-010 Szczecin, Poland.
Karolina Kędzierska-KapuzaDepartment of Gastroenterological Surgery and Transplantology, National Medical Institute, Ministry of Interior Affairs and Administration, 02-507 Warsaw, Poland.ORCID 0000-0001-5853-2147

Funding

Pomeranian Medical University WMS/2017/S/2024
6 · The paper itself

Abstract

Chronic immunosuppressive therapy is currently the only effective method to prevent acute rejection of a transplanted organ. Unfortunately, the expected effect of treatment brings a number of grave side effects, one of the most serious being cardiovascular complications. In our study, we wanted to investigate how treatment with commonly used immunosuppressive drugs affects the occurrence of programmed cardiac cell death. For this purpose, five groups of rats were treated with different triple immunosuppressive regimens. Cardiac tissue fragments were subjected to the TUNEL assay to visualize apoptotic cells. The expression of Bcl-2 protein, Bax protein, caspase 3 and caspase 9 was also assessed. This study indicates that all immunosuppressive protocols used chronically at therapeutic doses result in an increased percentage of cells undergoing apoptosis in rat heart tissue. The greatest changes were recorded in the TMG (rats treated with tacrolimus, mycophenolate mofetil and glucocorticosteroids) and CMG (rats treated with cyclosporin A, mycophenolate mofetil and glucocorticosteroids) groups. The TRG (rats treated with rapamycin, tacrolimus and glucocorticosteroids) group showed the lowest percentage of apoptotic cells. The internal apoptosis pathway was confirmed only in the TMG group; in the remaining groups, the results indicate programmed cell death via the receptor pathway.

Indexed as

apoptosiscalcineurin inhibitorscaspasesheart failureimmunosuppressive drugsmycophenolate mofetiltacrolimus

Identifiers

PMID39338354
PMCPMC11435130

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.