Evidence map›Paper›PMID 39344191›Full record

ArticleJournal of cellular physiology2024

Dissecting SOX9 dynamics reveals its differential regulation in osteoarthritis.

Kannan Govindaraj, Sakshi Kannan, Rodrigo Coutinho de Almeida, Lucas Jansen Klomp, Marcel Karperien, Ingrid Meulenbelt, Janine N Post

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular physiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Kannan GovindarajDepartment of Developmental Bioengineering, Faculty of Science and Technology, Technical Medical Center, University of Twente, Enschede, The Netherlands.ORCID 0000-0001-9030-8735
Sakshi KannanDepartment of Developmental Bioengineering, Faculty of Science and Technology, Technical Medical Center, University of Twente, Enschede, The Netherlands.
Rodrigo Coutinho de AlmeidaDepartment of Biomedical Data Sciences, Section Molecular Epidemiology, Leiden University Medical Center, Leiden, The Netherlands.
Lucas Jansen KlompDepartment of Developmental Bioengineering, Faculty of Science and Technology, Technical Medical Center, University of Twente, Enschede, The Netherlands.
Marcel KarperienDepartment of Developmental Bioengineering, Faculty of Science and Technology, Technical Medical Center, University of Twente, Enschede, The Netherlands.
Ingrid MeulenbeltDepartment of Biomedical Data Sciences, Section Molecular Epidemiology, Leiden University Medical Center, Leiden, The Netherlands.
Janine N PostDepartment of Developmental Bioengineering, Faculty of Science and Technology, Technical Medical Center, University of Twente, Enschede, The Netherlands.

Funding

Dutch Arthritis Society (ReumaNederland) 18-1-208Dutch Research Council (NWO) OCENW.GROOT.2019.079European Commission-Marie Curie 101109659
6 · The paper itself

Abstract

The transcription factor SOX9 is integral to tissue homeostasis and is implicated in skeletal malformation, campomelic dysplasia, and osteoarthritis (OA). Despite extensive research, the complete regulatory landscape of SOX9 transcriptional activity, interconnected with signaling pathways (TGFβ, WNT, BMP, IHH, NFκB, and HIF), remains challenging to decipher. This study focuses on elucidating SOX9 signaling in OA pathology using Fluorescence Recovery After Photobleaching (FRAP) to assess SOX9 activity directly in live human primary chondrocytes (hPCs). Single cell FRAP data revealed two distinct subpopulations with differential SOX9 dynamics, showing varied distribution between healthy and OA hPCs. Moreover, inherently elevated SOX9-DNA binding was observed in healthy hPCs compared to preserved and OA counterparts. Anabolic factors (BMP7 and GREM1) and catabolic inhibitors (DKK1 and FRZb) were found to modulate SOX9 transcriptional activity in OA-hPCs. These findings provide valuable insights into the intricate regulation of SOX9 signaling in OA, suggesting potential therapeutic avenues for modulating SOX9 activity in diseased states.

Indexed as

ChondrocytesIntercellular Signaling Peptides and ProteinsOsteoarthritisSignal TransductionSOX9 Transcription FactorAgedCells, CulturedFrizzled-Related ProteinsGene Expression RegulationHumansIntracellular Signaling Peptides and ProteinsDKK1 protein, humanFrizzled-Related ProteinsGREM1 protein, humanIntercellular Signaling Peptides and ProteinsIntracellular Signaling Peptides and ProteinsSOX9 protein, humanSOX9 Transcription FactorcartilageCellular heterogeneityDNA localizationFRAPProtein dynamicsTranscription factor dynamics

Identifiers

PMID39344191
PMCPMC11649970

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.