Evidence map›Paper›PMID 39349745›Full record

ArticleScientific reports2024

A live single-cell reporter system reveals drug-induced plasticity of a cancer stem cell-like population in cholangiocarcinoma.

Krittiyabhorn Kongtanawanich, Sunisa Prasopporn, Supawan Jamnongsong, Nontaphat Thongsin, Tongchai Payungwong, Seiji Okada, Marianne Hokland, Methichit Wattanapanitch, Siwanon Jirawatnotai

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Krittiyabhorn KongtanawanichDepartment of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Sunisa PrasoppornDepartment of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Supawan JamnongsongDepartment of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Nontaphat ThongsinSiriraj Center for Regenerative Medicine, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Tongchai PayungwongDepartment of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Seiji OkadaDivision of Hematopoiesis, Joint Research Center for Human Retrovirus Infection, Kumamoto University, Kumamoto, Japan.
Marianne HoklandDepartment of Biomedicine, Aarhus University, Aarhus, Denmark.
Methichit WattanapanitchSiriraj Center for Regenerative Medicine, Research Department, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Siwanon JirawatnotaiDepartment of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand. siwanon.jir@mahidol.ac.th.

Funding

Mahidol University Basic Research Fund FF2022National Research Council of Thailand (NRCT) N41A640162the Royal Golden Jubilee Ph.D. Scholarship Program (RGJ-PHD) (Grant No. PHD/0060/2561)
6 · The paper itself

Abstract

Cancer stem cells (CSC) play an important role in carcinogenesis and are acknowledged to be responsible for chemoresistance in cholangiocarcinoma (CCA). Studying CCA CSC has been challenging, due to lack of consensus CSC markers, and to their plastic nature. Since dual expression of the core pluripotent factors SOX2/OCT4 has been shown to correlate with poor outcome in CCA patients, we selected the SOX2/OCT4 activating short half-life GFP-based live reporter (SORE6-dsCopGFP) to study CSC dynamics at the single-cell level. Transduction of five human CCA cell lines resulted in the expression of 1.8-13.1% GFP-positive (SORE6

Indexed as

Bile Duct NeoplasmsCholangiocarcinomaNeoplastic Stem CellsAnimalsAntineoplastic AgentsCell Line, TumorCell PlasticityDrug Resistance, NeoplasmGenes, ReporterHumansMiceOctamer Transcription Factor-3Single-Cell AnalysisSOXB1 Transcription FactorsAntineoplastic AgentsOctamer Transcription Factor-3POU5F1 protein, humanSOX2 protein, humanSOXB1 Transcription FactorsCancer stem cellCDK4/6 inhibitorCholangiocarcinomaDrug resistanceLive reporter

Identifiers

PMID39349745
PMCPMC11442615

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.