Evidence map›Paper›PMID 39350266›Full record

ArticleBMC medical genomics2024

Single cell RNA sequencing provides novel cellular transcriptional profiles and underlying pathogenesis of presbycusis.

Juhong Zhang, Lili Xiang, Wenfang Sun, Menglong Feng, Zhiji Chen, Hailan Mo, Haizhu Ma, Li Yang, Shaojing Kuang, Yaqin Hu and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC medical genomics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Juhong ZhangDepartment of Otorhinolaryngology Head and Neck Surgery, Chongqing General Hospital, No.118, Xingguang Avenue, Liangjiang New Area, Chongqing, 401147, China.
Lili XiangDepartment of Otorhinolaryngology Head and Neck Surgery and Hearing Screening and Diagnosis Center, Jinan Maternity and Child Care Hospital, Jinan, Shandong, China.
Wenfang SunDepartment of Otorhinolaryngology Head and Neck Surgery, Chongqing General Hospital, No.118, Xingguang Avenue, Liangjiang New Area, Chongqing, 401147, China.
Menglong FengDepartment of Otorhinolaryngology Head and Neck Surgery, Chongqing General Hospital, No.118, Xingguang Avenue, Liangjiang New Area, Chongqing, 401147, China.
Zhiji ChenDepartment of Otorhinolaryngology Head and Neck Surgery, Chongqing General Hospital, No.118, Xingguang Avenue, Liangjiang New Area, Chongqing, 401147, China.
Hailan MoDepartment of Otorhinolaryngology Head and Neck Surgery, Chongqing General Hospital, No.118, Xingguang Avenue, Liangjiang New Area, Chongqing, 401147, China.
Haizhu MaDepartment of Otorhinolaryngology Head and Neck Surgery, Chongqing General Hospital, No.118, Xingguang Avenue, Liangjiang New Area, Chongqing, 401147, China.
Li YangDepartment of Otorhinolaryngology Head and Neck Surgery, Chongqing General Hospital, No.118, Xingguang Avenue, Liangjiang New Area, Chongqing, 401147, China.
Shaojing KuangDepartment of Otorhinolaryngology Head and Neck Surgery, Chongqing General Hospital, No.118, Xingguang Avenue, Liangjiang New Area, Chongqing, 401147, China.
Yaqin HuDepartment of Otorhinolaryngology Head and Neck Surgery, Chongqing General Hospital, No.118, Xingguang Avenue, Liangjiang New Area, Chongqing, 401147, China.
Jialin GuoDepartment of Otorhinolaryngology Head and Neck Surgery, Chongqing General Hospital, No.118, Xingguang Avenue, Liangjiang New Area, Chongqing, 401147, China.
Yijun LiDepartment of Otorhinolaryngology Head and Neck Surgery, Chongqing General Hospital, No.118, Xingguang Avenue, Liangjiang New Area, Chongqing, 401147, China.
Wei YuanDepartment of Otorhinolaryngology Head and Neck Surgery, Chongqing General Hospital, No.118, Xingguang Avenue, Liangjiang New Area, Chongqing, 401147, China. yuanwei@ucas.ac.cn.

Funding

Chongqing medical scientific research general project (Joint project of Chongqing Health Commission and Science and Technology Bureau) cstc2021jcyj-bshX0026Chongqing medical scientific research major project (Joint project of Chongqing Health Commission and Science and Technology Bureau) 2022DBXM006Chongqing Talent Program. Innovative leading talents in the medical field 2021Chongqing Technology innovation and application development special project CSTB2023TIAD-KPX0059Chongqing young and middle-aged medical excellence team The 1st batchGeneral Program of Chongqing Natural Science Foundation cstc2021jcyj-msxmX0128the Young and middle-aged medical high-end talents in Chongqing The 7th batch
6 · The paper itself

Abstract

Age-related hearing loss (ARHL) or presbycusis is associated with irreversible progressive damage in the inner ear, where the sound is transduced into electrical signal; but the detailed mechanism remains unclear. Here, we sought to determine the potential molecular mechanism involved in the pathogeneses of ARHL with bioinformatics methods. A single-cell transcriptome sequencing study was performed on the cochlear samples from young and aged mice. Detection of identified cell type marker allowed us to screen 18 transcriptional clusters, including myeloid cells, epithelial cells, B cells, endothelial cells, fibroblasts, T cells, inner pillar cells, neurons, inner phalangeal cells, and red blood cells. Cell-cell communications were analyzed between young and aged cochlear tissue samples by using the latest integration algorithms Cellchat. A total of 56 differentially expressed genes were screened between the two groups. Functional enrichment analysis showed these genes were mainly involved in immune, oxidative stress, apoptosis, and metabolic processes. The expression levels of crucial genes in cochlear tissues were further verified by immunohistochemistry. Overall, this study provides new theoretical support for the development of clinical therapeutic drugs.

Indexed as

PresbycusisSingle-Cell AnalysisAnimalsCochleaGene Expression ProfilingMiceSequence Analysis, RNATranscriptomeCochleaDifferentially expressed genesPresbycusisscRNA-seq

Identifiers

PMID39350266
PMCPMC11441099

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.