Evidence map›Paper›PMID 39354504›Full record

ArticleBMC oral health2024

Decellularized extracellular matrix derived from dental pulp stem cells promotes gingival fibroblast adhesion and migration.

Nunthawan Nowwarote, Zakaria Chahlaoui, Stephane Petit, Lucas T Duong, Florent Dingli, Damarys Loew, Ajjima Chansaenroj, Chatvadee Kornsuthisopon, Thanaphum Osathanon, Francois Come Ferre and 1 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in BMC oral health, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Nunthawan NowwaroteCentre de Recherche des Cordeliers, Molecular Oral Pathophysiology, INSERM UMRS 1138, Université Paris Cité, Sorbonne Université, Paris, 75006, France. nunthawan.nowwarote@u-paris.fr.
Zakaria ChahlaouiCentre de Recherche des Cordeliers, Molecular Oral Pathophysiology, INSERM UMRS 1138, Université Paris Cité, Sorbonne Université, Paris, 75006, France.
Stephane PetitCentre de Recherche des Cordeliers, Molecular Oral Pathophysiology, INSERM UMRS 1138, Université Paris Cité, Sorbonne Université, Paris, 75006, France.
Lucas T DuongCentre de Recherche des Cordeliers, Molecular Oral Pathophysiology, INSERM UMRS 1138, Université Paris Cité, Sorbonne Université, Paris, 75006, France.
Florent DingliCentre de Recherche, CurieCoreTech Spectrométrie de Masse Protéomique, Institut Curie, PSL Research University, Paris, France.
Damarys LoewCentre de Recherche, CurieCoreTech Spectrométrie de Masse Protéomique, Institut Curie, PSL Research University, Paris, France.
Ajjima ChansaenrojCenter of Excellence for Dental Stem Cell Biology, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330, Thailand.
Chatvadee KornsuthisoponCenter of Excellence for Dental Stem Cell Biology, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330, Thailand. Chatvadee.k@chula.ac.th.
Thanaphum OsathanonCenter of Excellence for Dental Stem Cell Biology, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330, Thailand.
Francois Come FerreCentre de Recherche des Cordeliers, Molecular Oral Pathophysiology, INSERM UMRS 1138, Université Paris Cité, Sorbonne Université, Paris, 75006, France.
Benjamin P J FournierCentre de Recherche des Cordeliers, Molecular Oral Pathophysiology, INSERM UMRS 1138, Université Paris Cité, Sorbonne Université, Paris, 75006, France.

Funding

Faculty Research Grant, the Faculty of Dentistry, Chulalongkorn University DRF 67024
6 · The paper itself

Abstract

backgroundDecellularized extracellular matrix (dECM) has been proposed as a useful source of biomimetic materials for regenerative medicine due to its biological properties that regulate cell behaviors. The present study aimed to investigate the influence of decellularized ECM derived from dental pulp stem cells (DPSCs) on gingival fibroblast (GF) cell behaviors. Cells were isolated from dental pulp and gingival tissues. ECM was derived from culturing dental pulp stem cells in growth medium supplemented with ascorbic acid. A bioinformatic database of the extracellular matrix was constructed using Metascape. GFs were reseeded onto dECM, and their adhesion, spreading, and organization were subsequently observed. The migration ability of the cells was determined using a scratch assay. Protein expression was evaluated using immunofluorescence staining.

resultsType 1 collagen and fibronectin were detected on the ECM and dECM derived from DPSCs. Negative phalloidin and nuclei were noted in the dECM. The proteomic database revealed enrichment of several proteins involved in ECM organization, ECM-receptor interaction, and focal adhesion. Compared with those on the controls, the GFs on the dECM exhibited more organized stress fibers. Furthermore, cultured GFs on dECM exhibited significantly enhanced migration and proliferation abilities. Interestingly, GFs seeded on dECM showed upregulation of FN1, ITGB3, and CTNNB1 mRNA levels.

conclusionsECM derived from DSPCs generates a crucial microenvironment for regulating GF adhesion, migration and proliferation. Therefore, decellularized ECM from DPSCs could serve as a matrix for oral tissue repair.

Indexed as

Cell AdhesionCell MovementDental PulpExtracellular MatrixFibroblastsGingivaStem CellsCell ProliferationCells, CulturedFibronectinsHumansFibronectinsCell adhesionCell migrationDecellularized extracellular matrixDental pulp stem cellsGingival fibroblasts

Identifiers

PMID39354504
PMCPMC11443845

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.