ArticleNature communications2024
Concordant inter-laboratory derived concentrations of ceramides in human plasma reference materials via authentic standards.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it.
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- A lipidomics roadmap: from basic research to societal challenges.Nature communications · 2026Review
- New Analytical Technologies to Resolve, Interpret, and Understand Lipid Complexity.Annual review of analytical chemistry (Palo Alto, Calif.) · 2026Review
- Lifespan trajectories of serum sphingolipids in a clinically healthy Swiss population, towards precision medicine in cardiometabolic health assessment.Communications medicine · 2026Article
- The ratio of circulatory levels of sphingolipids to steroids predicts asthma exacerbations.Nature communications · 2026Article
- Biomarkers of Cardiac Metabolic Flexibility in Health, HFrEF and HFpEF.International journal of molecular sciences · 2026Review
- Chronic Kidney Disease, Circulating Ceramides, and Coronary Microvascular Dysfunction: Insights from the CRUISE-MET Study.Cardiorenal medicine · 2026Observational
- Benzoyl chloride derivatization improves selectivity and sensitivity of lipidomic quantitation in human serum of pancreatic cancer patients using RP-UHPLC/MS/MS.Analytical and bioanalytical chemistry · 2026Article
- Brief Evaluation of Olink Reveal Proximity Extension Assay for High-Throughput Proteomics: A Case Study Using NIST SRM 1950 and Two Spike-In Protein Standards.Journal of proteome research · 2025Article
- A standardized framework for circulating blood proteomics.Nature genetics · 2025Review
- Ceramides in cardiovascular disease: emerging role as independent risk predictors and novel therapeutic targets.Cardiovascular research · 2025Review
- Hypoxic Status in COPD and ARDS Patients: Impact on Lipid Signature.International journal of molecular sciences · 2025Article
- The bioactive sphingolipid playbook. A primer for the uninitiated as well as sphingolipidologists.Journal of lipid research · 2025Review
- Clinical lipidomics reveals high individuality and sex specificity of circulatory lipid signatures: a prospective healthy population study.Journal of lipid research · 2025Article
- Quantitative Lipid Analysis of Extracellular Vesicle Preparations: A Perspective.Journal of extracellular vesicles · 2025Article
- Don't Be Surprised When These Surprise You: Some Infrequently Studied Sphingoid Bases, Metabolites, and Factors That Should Be Kept in Mind During Sphingolipidomic Studies.International journal of molecular sciences · 2025Review
- Advancements in Mass Spectrometry-Based Targeted Metabolomics and Lipidomics: Implications for Clinical Research.Molecules (Basel, Switzerland) · 2024Review
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Authors and funding
81 authors.
Federico Torta *Singapore Lipidomics Incubator, Life Sciences Institute, National University of Singapore, Singapore, 117456, Singapore.
Nils Hoffmann *Institute for Bio- and Geosciences (IBG-5), Forschungszentrum Jülich GmbH, 52428, Jülich, Germany.
Bo Burla *Singapore Lipidomics Incubator, Life Sciences Institute, National University of Singapore, Singapore, 117456, Singapore.ORCID 0000-0002-5918-3249
Irina AlecuNeural Regeneration Laboratory, Ottawa Institute of Systems Biology, Ottawa Brain and Mind Research Institute, Department of Biochemistry, Microbiology, and Immunology, and Department of Chemistry, Centre for Catalysis Research and Innovation, University of Ottawa, Ottawa, K1H 8M5, Canada.
Makoto AritaRIKEN Center for Integrative Medical Sciences, Yokohama, Japan.ORCID 0000-0001-9902-0463
Takeshi BambaDivision of Metabolomics Center, Medical Research Center for High Depth Omics, Medical Institute of Bioregulation, Kyushu University, 3‑1‑1, Maidashi, Higashi‑ku, Fukuoka, 812‑8582, Japan.ORCID 0000-0002-7442-2738
Steffany A L BennettNeural Regeneration Laboratory, Ottawa Institute of Systems Biology, Ottawa Brain and Mind Research Institute, Department of Biochemistry, Microbiology, and Immunology, and Department of Chemistry, Centre for Catalysis Research and Innovation, University of Ottawa, Ottawa, K1H 8M5, Canada.ORCID 0000-0001-7944-5800
Justine Bertrand-MichelMetaboHUBMetaToul Facility, I2MC U1297 Inserm, Toulouse, France.
Britta BrüggerHeidelberg University Biochemistry Center (BZH), Im Neuenheimer Feld 328, 69120, Heidelberg, Germany.ORCID 0000-0002-3477-8270
Mónica P CalaMetabolomics Core Facility-MetCore, Universidad de los Andes, Bogotá, 111711, Colombia.
Dolores Camacho-MuñozLaboratory for Lipidomics and Lipid Biology, Division of Pharmacy and Optometry, School of Health Sciences, Faculty of Biology Medicine and Health, The University of Manchester, Manchester Academic Health Science Centre, Manchester, M13 9NT, United Kingdom.
Antonio ChecaUnit of Integrative Metabolomics, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.
Michael ChenDepartment of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada.
Michaela ChocholouškováDepartment of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10, Pardubice, Czech Republic.
Michelle CinelBaker Heart and Diabetes Institute, Melbourne, VIC, 3004, Australia.
Emeline Chu-VanUniversité Paris-Saclay, CEA, INRAE, Département Médicaments et Technologies pour la Santé (DMTS), MetaboHUB, F-91191, Gif sur Yvette, France.
Benoit ColschUniversité Paris-Saclay, CEA, INRAE, Département Médicaments et Technologies pour la Santé (DMTS), MetaboHUB, F-91191, Gif sur Yvette, France.ORCID 0000-0002-8023-504X
Cristina ComanDepartment of Analytical Chemistry, University of Vienna, Vienna, Austria.
Lisa ConnellAvanti Polar Lipids, Alabaster, AL, USA.
Bebiana C SousaBabraham Institute, Babraham Research Campus, Cambridge, MA, CB22 3AT, USA.
Alex M DickensTurku Bioscience Centre, University of Turku and Åbo Akademi University, 20520, Turku, Finland.ORCID 0000-0002-3178-8449
Maria FedorovaInstitute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, University of Leipzig, 04013, Leipzig, Germany.ORCID 0000-0002-4692-3885
Finnur Freyr EiríkssonFaculty of Pharmaceutical Sciences, University of Iceland, Reykjavik, Iceland.
Hector Gallart-AyalaMetabolomics Platform, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland.ORCID 0000-0003-2333-0646
Mohan GhorasainiCenter for Proteomics and Metabolomics, Leiden University Medical Center, 2333ZA, Leiden, The Netherlands.
Martin GieraCenter for Proteomics and Metabolomics, Leiden University Medical Center, 2333ZA, Leiden, The Netherlands.
Xue Li GuanLee Kong Chian School of Medicine, Nanyang Technological University, Singapore, 636921, Singapore.ORCID 0000-0002-5189-8178
Mark HaidMetabolomics and Proteomics Core, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, 85764, Germany.ORCID 0000-0001-6118-1333
Thomas HankemeierMetabolomics and Analytics Centre, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands.
Amy HarmsMetabolomics and Analytics Centre, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands.ORCID 0000-0002-2931-4295
Marcus HöringUniversity Hospital of Regensburg, Institute of Clinical Chemistry and Laboratory Medicine, Franz-Josef-Strauß-Allee 11, 93053, Regensburg, Germany.
Michal HolčapekDepartment of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10, Pardubice, Czech Republic.ORCID 0000-0003-3978-1249
Thorsten HornemannInstitute of Clinical Chemistry, University Zurich, 8952, Schlieren, Switzerland.ORCID 0000-0002-4218-1087
Chunxiu HuState Key Laboratory of Medical Proteomics, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
Andreas J HülsmeierInstitute of Clinical Chemistry, University Zurich, 8952, Schlieren, Switzerland.ORCID 0000-0001-6987-8979
Kevin HuynhBaker Heart and Diabetes Institute, Melbourne, VIC, 3004, Australia.ORCID 0000-0001-6170-2207
Christina M JonesChemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD, 20899, USA.
Julijana IvanisevicMetabolomics Platform, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland.ORCID 0000-0001-8267-2705
Yoshihiro IzumiDivision of Metabolomics Center, Medical Research Center for High Depth Omics, Medical Institute of Bioregulation, Kyushu University, 3‑1‑1, Maidashi, Higashi‑ku, Fukuoka, 812‑8582, Japan.ORCID 0000-0003-4608-4652
Harald C KöfelerCore Facility Mass Spectrometry, Medical University of Graz, 8010, Graz, Austria.ORCID 0000-0002-2725-9616
Sin Man LamLipidALL Technologies, Changzhou, 213000, Jiangshu, China.ORCID 0000-0001-9037-904X
Mike LangeInstitute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, University of Leipzig, 04013, Leipzig, Germany.
Jong Cheol LeeDepartment of Chemistry, Yonsei University, Seoul, 03722, South Korea.
Gerhard LiebischUniversity Hospital of Regensburg, Institute of Clinical Chemistry and Laboratory Medicine, Franz-Josef-Strauß-Allee 11, 93053, Regensburg, Germany.ORCID 0000-0003-4886-0811
Katrice LippaChemical Science Division, National Institute of Standards and Technology, Gaithersburg, MD, 20899, USA.
Andrea F Lopez-ClavijoBabraham Institute, Babraham Research Campus, Cambridge, MA, CB22 3AT, USA.ORCID 0000-0002-6249-7767
Malena ManziCentro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2390, C1425FQD, Ciudad de Buenos Aires, Argentina.
Manuela R MartinefskiCentro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2390, C1425FQD, Ciudad de Buenos Aires, Argentina.ORCID 0000-0002-4501-3783
Raviswamy G H MathNational Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, Karnataka, 560065, India.
Satyajit MayorNational Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, Karnataka, 560065, India.ORCID 0000-0001-9842-6963
Peter J MeikleBaker Heart and Diabetes Institute, Melbourne, VIC, 3004, Australia.ORCID 0000-0002-2593-4665
María Eugenia MongeCentro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2390, C1425FQD, Ciudad de Buenos Aires, Argentina.
Myeong Hee MoonDepartment of Chemistry, Yonsei University, Seoul, 03722, South Korea.ORCID 0000-0002-5454-2601
Sneha MuralidharanSingapore Lipidomics Incubator, Life Sciences Institute, National University of Singapore, Singapore, 117456, Singapore.
Anna NicolaouLaboratory for Lipidomics and Lipid Biology, Division of Pharmacy and Optometry, School of Health Sciences, Faculty of Biology Medicine and Health, The University of Manchester, Manchester Academic Health Science Centre, Manchester, M13 9NT, United Kingdom.ORCID 0000-0002-1314-413X
Thao Nguyen-TranNeural Regeneration Laboratory, Ottawa Institute of Systems Biology, Ottawa Brain and Mind Research Institute, Department of Biochemistry, Microbiology, and Immunology, and Department of Chemistry, Centre for Catalysis Research and Innovation, University of Ottawa, Ottawa, K1H 8M5, Canada.ORCID 0000-0002-5507-618X
Valerie B O'DonnellSystems Immunity Research Institute, School of Medicine, Cardiff University, Heath Park, Cardiff, CF14 4XN, UK.ORCID 0000-0003-4089-8460
Matej OrešičTurku Bioscience Centre, University of Turku and Åbo Akademi University, 20520, Turku, Finland.ORCID 0000-0002-2856-9165
Arvind RamanathanInstitute for Stem Cell Science and Regenerative Medicine, 560065, Bangalore, India.ORCID 0000-0002-0375-3740
Fabien RiolsMetabolomics and Proteomics Core, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, 85764, Germany.
Daisuke SaigusaLaboratory of Biomedical and Analytical Sciences, Faculty of Pharma-Science, Teikyo University, 2-11-1 Kaga, Itabashi-ku, Tokyo, 173-8605, Japan.
Tracey B SchockChemical Science Division, National Institute of Standards and Technology, Charleston, SC, 29412, USA.ORCID 0000-0002-1808-7816
Heidi Schwartz-ZimmermannChristian Doppler Laboratory for Innovative Gut Health Concepts of Livestock, Institute of Bioanalytics and Agro-Metabolomics, Department of Agrobiotechnology (IFATulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Konrad-Lorenz-Str. 20, 3430, Tulln, Austria.
Guanghou ShuiState Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.ORCID 0000-0002-1621-9643
Madhulika SinghMetabolomics and Analytics Centre, Leiden Academic Centre for Drug Research, Leiden University, Leiden, The Netherlands.ORCID 0000-0002-7433-9352
Masatomo TakahashiDivision of Metabolomics Center, Medical Research Center for High Depth Omics, Medical Institute of Bioregulation, Kyushu University, 3‑1‑1, Maidashi, Higashi‑ku, Fukuoka, 812‑8582, Japan.ORCID 0000-0002-2493-2684
Margrét ThorsteinsdóttirFaculty of Pharmaceutical Sciences, University of Iceland, Reykjavik, Iceland.
Noriyuki TomiyasuDivision of Metabolomics Center, Medical Research Center for High Depth Omics, Medical Institute of Bioregulation, Kyushu University, 3‑1‑1, Maidashi, Higashi‑ku, Fukuoka, 812‑8582, Japan.
Anthony TournadreMetaboHUBMetaToul Facility, I2MC U1297 Inserm, Toulouse, France.
Hiroshi TsugawaRIKEN Center for Integrative Medical Sciences, Yokohama, Japan.ORCID 0000-0002-2015-3958
Victoria J TyrrellSystems Immunity Research Institute, School of Medicine, Cardiff University, Heath Park, Cardiff, CF14 4XN, UK.
Grace van der GugtenSt. Paul's Hospital, Department of Pathology and Laboratory Medicine, Vancouver, BC, Canada.
Michael O WakelamBabraham Institute, Babraham Research Campus, Cambridge, MA, CB22 3AT, USA.
Craig E WheelockUnit of Integrative Metabolomics, Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.ORCID 0000-0002-8113-0653
Denise WolrabDepartment of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10, Pardubice, Czech Republic.
Guowang XuState Key Laboratory of Medical Proteomics, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.ORCID 0000-0003-4298-3554
Tianrun XuState Key Laboratory of Medical Proteomics, CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China.
John A BowdenDepartment of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL, 32610, USA.
Kim EkroosLipidomics Consulting Ltd., Espoo, Finland. kim@lipidomicsconsulting.com.ORCID 0000-0001-6810-4736
Robert AhrendsDepartment of Analytical Chemistry, University of Vienna, Vienna, Austria. robert.ahrends@univie.ac.at.ORCID 0000-0003-0232-3375
Markus R WenkSingapore Lipidomics Incubator, Life Sciences Institute, National University of Singapore, Singapore, 117456, Singapore. mwenk@hbku.edu.qa.ORCID 0000-0001-5447-7881
Funding
British Heart Foundation PG/2019/34923
Abstract
In this community effort, we compare measurements between 34 laboratories from 19 countries, utilizing mixtures of labelled authentic synthetic standards, to quantify by mass spectrometry four clinically used ceramide species in the NIST (National Institute of Standards and Technology) human blood plasma Standard Reference Material (SRM) 1950, as well as a set of candidate plasma reference materials (RM 8231). Participants either utilized a provided validated method and/or their method of choice. Mean concentration values, and intra- and inter-laboratory coefficients of variation (CV) were calculated using single-point and multi-point calibrations, respectively. These results are the most precise (intra-laboratory CVs ≤ 4.2%) and concordant (inter-laboratory CVs < 14%) community-derived absolute concentration values reported to date for four clinically used ceramides in the commonly analyzed SRM 1950. We demonstrate that calibration using authentic labelled standards dramatically reduces data variability. Furthermore, we show how the use of shared RM can correct systematic quantitative biases and help in harmonizing lipidomics. Collectively, the results from the present study provide a significant knowledge base for translation of lipidomic technologies to future clinical applications that might require the determination of reference intervals (RIs) in various human populations or might need to estimate reference change values (RCV), when analytical variability is a key factor for recall during multiple testing of individuals.
Indexed as
CeramidesLaboratoriesReference StandardsCalibrationHumansLipidomicsMass SpectrometryReproducibility of ResultsCeramides
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