Evidence map›Paper›PMID 39363087›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Golden Gate Cloning for the Standardized Assembly of Gene Elements with Modular Cloning in Chlamydomonas.

Peter Emelin, Sarah Abdul-Mawla, Felix Willmund

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Peter Emelin *Molecular Plant Sciences, Center for Synthetic Microbiology (SYNMIKRO), Philipps-University Marburg, Marburg, Germany.
Sarah Abdul-Mawla *Molecular Plant Sciences, Center for Synthetic Microbiology (SYNMIKRO), Philipps-University Marburg, Marburg, Germany.
Felix WillmundMolecular Plant Sciences, Center for Synthetic Microbiology (SYNMIKRO), Philipps-University Marburg, Marburg, Germany. willmund@staff.uni-marburg.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Modern synthetic biology requires fast and efficient cloning strategies for the assembly of new transcription units or entire pathways. Modular Cloning (MoClo) is a standardized synthetic biology workflow, which has tremendously simplified the assembly of genetic elements for transgene expression. MoClo is based on Golden Gate Assembly and allows to combine genetic elements of a library through a hierarchical syntax-driven pipeline. Here we describe the assembly of a genetic cassette for transgene expression in the single-celled model alga Chlamydomonas reinhardtii.

Indexed as

Chlamydomonas reinhardtiiCloning, MolecularSynthetic BiologyChlamydomonasGene LibraryGenetic EngineeringGenetic VectorsTransgenesChlamydomonas reinhardtiiGolden Gate AssemblyModular cloningSynthetic biology

Identifiers

PMID39363087

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.