Evidence map›Paper›PMID 39367476›Full record

ArticleMicrobial cell factories2024

Production of α-ketoisovalerate with whey powder by systemic metabolic engineering of Klebsiella oxytoca.

Weikang Sun, Shuo Wang, Xiaoxu Tan, Leilei Guo, Wei Liu, Wenjia Tian, Hui Zhang, Tianyi Jiang, Wensi Meng, Yidong Liu and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial cell factories, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Multistep metabolic engineeredSynthetic and systems biotechnology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Weikang SunState Key Laboratory of Microbial Technology, Shandong University, NO.72 Binhai Road, Qingdao, 266237, China.
Shuo WangState Key Laboratory of Microbial Technology, Shandong University, NO.72 Binhai Road, Qingdao, 266237, China.
Xiaoxu TanState Key Laboratory of Microbial Technology, Shandong University, NO.72 Binhai Road, Qingdao, 266237, China.
Leilei GuoState Key Laboratory of Microbial Technology, Shandong University, NO.72 Binhai Road, Qingdao, 266237, China.
Wei LiuState Key Laboratory of Microbial Technology, Shandong University, NO.72 Binhai Road, Qingdao, 266237, China.
Wenjia TianState Key Laboratory of Microbial Technology, Shandong University, NO.72 Binhai Road, Qingdao, 266237, China.
Hui ZhangState Key Laboratory of Microbial Technology, Shandong University, NO.72 Binhai Road, Qingdao, 266237, China.
Tianyi JiangSchool of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan, 250101, China.
Wensi MengState Key Laboratory of Microbial Technology, Shandong University, NO.72 Binhai Road, Qingdao, 266237, China.
Yidong LiuState Key Laboratory of Microbial Technology, Shandong University, NO.72 Binhai Road, Qingdao, 266237, China.
Zhaoqi KangState Key Laboratory of Microbial Technology, Shandong University, NO.72 Binhai Road, Qingdao, 266237, China.
Chuanjuan LüState Key Laboratory of Microbial Technology, Shandong University, NO.72 Binhai Road, Qingdao, 266237, China.
Chao GaoState Key Laboratory of Microbial Technology, Shandong University, NO.72 Binhai Road, Qingdao, 266237, China.
Ping XuState Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, 200240, China.
Cuiqing MaState Key Laboratory of Microbial Technology, Shandong University, NO.72 Binhai Road, Qingdao, 266237, China. macq@sdu.edu.cn.

Funding

China Postdoctoral Science Foundation 2023M742085National Natural Science Foundation of China 32300045Postdoctoral Innovation Program of Shandong Province No. SDCX-ZG-202400150Qingdao Postdoctoral Research Project QDBSH20230201011State Key Laboratory of Microbial Technology SKLMTFCP-2023-03State Key Laboratory of Microbial Technology Open Projects Fund M2023-02the National Key R&D Program of China 2022YFC2106000Youth Program of Natural Science Foundation of Qingdao City 23-2-1-31-zyyd-jchYouth Program of Natural Science Foundation of Shandong Province ZR2022QC092
6 · The paper itself

Abstract

backgroundWhey, which has high biochemical oxygen demand and chemical oxygen demand, is mass-produced as a major by-product of the dairying industry. Microbial fermentation using whey as the carbon source may convert this potential pollutant into value-added products. This study investigated the potential of using whey powder to produce α-ketoisovalerate, an important platform chemical.

resultsKlebsiella oxytoca VKO-9, an efficient L-valine producing strain belonging to Risk Group 1 organism, was selected for the production of α-ketoisovalerate. The leucine dehydrogenase and branched-chain α-keto acid dehydrogenase, which catalyzed the reductive amination and oxidative decarboxylation of α-ketoisovalerate, respectively, were inactivated to enhance the accumulation of α-ketoisovalerate. The production of α-ketoisovalerate was also improved through overexpressing α-acetolactate synthase responsible for pyruvate polymerization and mutant acetohydroxyacid isomeroreductase related to α-acetolactate reduction. The obtained strain K. oxytoca KIV-7 produced 37.3 g/L of α-ketoisovalerate from lactose, the major utilizable carbohydrate in whey. In addition, K. oxytoca KIV-7 also produced α-ketoisovalerate from whey powder with a concentration of 40.7 g/L and a yield of 0.418 g/g.

conclusionThe process introduced in this study enabled efficient α-ketoisovalerate production from low-cost substrate whey powder. Since the key genes for α-ketoisovalerate generation were integrated in genome of K. oxytoca KIV-7 and constitutively expressed, this strain is promising in stable α-ketoisovalerate fermentation and can be used as a chassis strain for α-ketoisovalerate derivatives production.

Indexed as

FermentationHemiterpenesKlebsiella oxytocaMetabolic EngineeringWheyAcetolactate SynthaseKeto AcidsPowdersAcetolactate Synthasealpha-ketoisovalerateHemiterpenesKeto AcidsPowdersKlebsiella oxytocaL-ValineMetabolic engineeringWhey powderα-Ketoisovalerate

Identifiers

PMID39367476
PMCPMC11452931

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.