Evidence map›Paper›PMID 39373389›Full record

ArticleDevelopment (Cambridge, England)2024

C. elegans epicuticlins define specific compartments in the apical extracellular matrix and function in wound repair.

Murugesan Pooranachithra, Erin M Jyo, Nicolas Brouilly, Nathalie Pujol, Andreas M Ernst, Andrew D Chisholm

Abstract read
In one paragraph

Article in Development (Cambridge, England), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. From nematode to Nobel: How community-shared resources fueled the rise ofProceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  3. MLT-11 is necessary forbioRxiv : the preprint server for biology · 2025
    Article
  4. Article
  5. Article
  6. Review
4 · The record

Corrections and comments

  • Update of
    2024
5 · Who and what money

Authors and funding

6 authors.

Murugesan PooranachithraDepartment of Cell and Developmental Biology, School of Biological Sciences, University of California San Diego, La Jolla, CA 92093, USA.ORCID 0000-0003-3506-7785
Erin M JyoDepartment of Cell and Developmental Biology, School of Biological Sciences, University of California San Diego, La Jolla, CA 92093, USA.
Nicolas BrouillyAix Marseille University, CNRS, IBDM, 13009, Marseille, France.ORCID 0000-0002-4584-0164
Nathalie PujolAix-Marseille Université, INSERM, CNRS, CIML, Turing Centre for Living Systems, 13009, Marseille, France.ORCID 0000-0001-8889-3197
Andreas M ErnstDepartment of Cell and Developmental Biology, School of Biological Sciences, University of California San Diego, La Jolla, CA 92093, USA.ORCID 0000-0001-7359-7184
Andrew D ChisholmDepartment of Cell and Developmental Biology, School of Biological Sciences, University of California San Diego, La Jolla, CA 92093, USA.ORCID 0000-0001-5091-0537

Funding

Center for C elegans AnatomyR24OD010943 · OD · ALBERT EINSTEIN COLLEGE OF MEDICINE, INC · PI DAVID H. HALL, Nathan Schroeder · 2012 to 2026
$9.6M
Enhancing and expanding the CGC Strain CollectionP40OD010440 · OD · UNIVERSITY OF MINNESOTA · PI Aric L Daul, Ann E. Rougvie · 2012 to 2026
$7.5M
Morphological Determinants of Intracellular Membrane CompartmentsR35GM142433 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Andreas Max Ernst · 2021 to 2026
$2.2M
Maintenance and Repair of the C. elegans SkinR35GM134970 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHISHOLM, ANDREW D · 2020 to 2024
$2.1M
Agence Nationale de la Recherche ANR-10-INBS-04Aix-Marseille Université AMX 19 IET 002NIGMS NIH HHS GM134970NIGMS NIH HHS GM142433NIGMS NIH HHS R35 GM134970NIGMS NIH HHS R35 GM142433NIH HHS P40 OD010440NIH HHS R24 OD010943
6 · The paper itself

Abstract

The apical extracellular matrix (aECM) of external epithelia often contains lipid-rich outer layers that contribute to permeability barrier function. The external aECM of nematodes is known as the cuticle and contains an external lipid-rich layer - the epicuticle. Epicuticlins are a family of tandem repeat cuticle proteins of unknown function. Here, we analyze the localization and function of the three C. elegans epicuticlins (EPIC proteins). EPIC-1 and EPIC-2 localize to the surface of the cuticle near the outer lipid layer, as well as to interfacial cuticles and adult-specific struts. EPIC-3 is expressed in dauer larvae and localizes to interfacial aECM in the buccal cavity. Skin wounding in the adult induces epic-3 expression, and EPIC proteins localize to wound sites. Null mutants lacking EPIC proteins are viable with reduced permeability barrier function and normal epicuticle lipid mobility. Loss of function in EPIC genes modifies the skin blistering phenotypes of Bli mutants and reduces survival after skin wounding. Our results suggest EPIC proteins define specific cortical compartments of the aECM and promote wound repair.

Indexed as

Caenorhabditis elegansCaenorhabditis elegans ProteinsExtracellular MatrixWound HealingAnimalsLarvaSkinCaenorhabditis elegans ProteinsDauer larvaDisordered proteinsEpitheliaLipid layersPermeability barrierTandem repeat proteins

Identifiers

PMID39373389
PMCPMC11529277

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.