Evidence map›Paper›PMID 39375866›Full record

ArticleACS sensors2024

Direct and Ultrasensitive Bioluminescent Detection of Intact Respiratory Viruses.

Alexander Gräwe, Harm van der Veer, Seino A K Jongkees, Jacky Flipse, Iebe Rossey, Robert P de Vries, Xavier Saelens, Maarten Merkx

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PubMed Full text at PMC Publisher
In one paragraph

Article in ACS sensors, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Alexander GräweLaboratory of Protein Engineering, Department of Biomedical Engineering and Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven 5600 MB, The Netherlands.ORCID 0000-0003-2892-278X
Harm van der VeerLaboratory of Protein Engineering, Department of Biomedical Engineering and Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven 5600 MB, The Netherlands.ORCID 0000-0002-4359-8079
Seino A K JongkeesDepartment of Chemistry and Pharmaceutical Sciences, Amsterdam Institute of Molecular and Life Sciences, Vrije Universiteit Amsterdam, Amsterdam 1081 HZ, The Netherlands.ORCID 0000-0002-4796-0557
Jacky FlipseLaboratory for Medical Microbiology and Immunology, Rijnstate Hospital, Arnhem 6880 AA, The Netherlands.ORCID 0000-0002-2195-5282
Iebe RosseyVIB Center for Medical Biotechnology, Department of Biochemistry and Microbiology, Ghent University, 9052 Zwijnaarde, Belgium.
Robert P de VriesDepartment of Chemical Biology and Drug Discovery, Utrecht Institute for Pharmaceutical Sciences, Utrecht 3584 CG, The Netherlands.ORCID 0000-0002-1586-4464
Xavier SaelensVIB Center for Medical Biotechnology, Department of Biochemistry and Microbiology, Ghent University, 9052 Zwijnaarde, Belgium.ORCID 0000-0002-3861-6965
Maarten MerkxLaboratory of Protein Engineering, Department of Biomedical Engineering and Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven 5600 MB, The Netherlands.ORCID 0000-0001-9484-3882

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Respiratory viruses such as SARS-CoV-2, influenza, and respiratory syncytial virus (RSV) represent pressing health risks. Rapid diagnostic tests for these viruses detect single antigens or nucleic acids, which do not necessarily correlate with the amount of the intact virus. Instead, specific detection of intact respiratory virus particles may be more effective at assessing the contagiousness of a patient. Here, we report GLOVID, a modular biosensor platform to detect intact virions against a background of "free" viral proteins in solution. Our approach harnesses the multivalent display of distinct proteins on the surface of a viral particle to template the reconstitution of a split luciferase, allowing specific, single-step detection of intact influenza A and RSV virions corresponding to 0.1-0.3 fM of genomic units. The protein ligation system used to assemble GLOVID sensors is compatible with a broad range of binding domains, including nanobodies, scFv fragments, and cyclic peptides, which allows straightforward adjustment of the sensor platform to target different viruses.

Indexed as

Biosensing TechniquesLuminescent MeasurementsSARS-CoV-2HumansInfluenza A virusRespiratory Syncytial Virusesbioluminescencebiosensorprotein engineeringprotein ligationRespiratory virusvirus diagnostics

Identifiers

PMID39375866
PMCPMC11519905

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.