Evidence map›Paper›PMID 39379628›Full record

ArticlePediatric research2025

Phlebotomy-induced anemia reduces oxygen-induced retinopathy severity and dampens retinal developmental transcriptomic pathways in rats.

Ellen C Ingolfsland, Mandkhai Molomjamts, Ann Foster, Haeyeon Lee, Heidi Roehrich, Amelia Morikuni, Husaam Qureishy, Phu V Tran, Linda K McLoon, Michael K Georgieff

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Article in Pediatric research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Ellen C IngolfslandDepartment of Pediatrics, Division of Neonatology, University of Minnesota Medical School, Minneapolis, MN, USA. eingolf@umn.edu.
Mandkhai MolomjamtsDepartment of Pediatrics, Division of Neonatology, University of Minnesota Medical School, Minneapolis, MN, USA.
Ann FosterDepartment of Pediatrics, Division of Neonatology, University of Minnesota Medical School, Minneapolis, MN, USA.
Haeyeon LeeDepartment of Pediatrics, Division of Neonatology, University of Minnesota Medical School, Minneapolis, MN, USA.
Heidi RoehrichDepartment of Ophthalmology and Visual Neurosciences, University of Minnesota Medical School, Minneapolis, MN, USA.
Amelia MorikuniDepartment of Pediatrics, Division of Neonatology, University of Minnesota Medical School, Minneapolis, MN, USA.
Husaam QureishyDepartment of Pediatrics, Division of Neonatology, University of Minnesota Medical School, Minneapolis, MN, USA.
Phu V TranDepartment of Pediatrics, Division of Neonatology, University of Minnesota Medical School, Minneapolis, MN, USA.
Linda K McLoonDepartment of Ophthalmology and Visual Neurosciences, University of Minnesota Medical School, Minneapolis, MN, USA.
Michael K GeorgieffDepartment of Pediatrics, Division of Neonatology, University of Minnesota Medical School, Minneapolis, MN, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPhlebotomy-induced-anemia (PIA), which induces tissue hypoxia and angiogenesis, occurs universally among infants at risk for severe retinopathy of prematurity (ROP). We hypothesized that PIA exacerbates pathologic retinal neovascularization in ROP.

methodsWe induced PIA to a hematocrit of 18% among rats undergoing the established 50/10 oxygen-induced retinopathy (OIR) model. Rats were euthanized at P15 and P20, during the avascular and neovascular phases of OIR, respectively. Retinal vascular morphometry, cytokine/chemokine concentrations, transcriptomes, and mRNA expression of angiogenic and iron-deficiency markers were compared to non-PIA controls.

resultsIn OIR, PIA decreased percent avascular area at P15 by 35%, percent neovascular area at P20 by 42%, and select pro-inflammatory cytokine/chemokine concentrations at both time points. At P20, PIA increased mRNA expression of angiopoietin 2/ vascular endothelial growth factor-A 2-fold and transferrin and transferrin receptor 5-fold. RNA sequencing showed dampened pathways of angiogenesis, inflammation, and neural development in anemic OIR females.

conclusionContrary to our hypothesis, PIA decreased OIR severity and retinal cytokine and chemokine levels and dampened transcriptomic pathways central to retinal vascular and neural development in neonatal rats. These data suggest PIA provides a protective effect from OIR. Further investigation into the functional effect of these molecular changes is warranted. IMPACT: This is the first preclinical study to investigate the impact of neonatal anemia on oxygen-induced retinopathy (OIR) outcomes. This study adds to the literature that anemia decreases neovascularization, decreases cytokine and chemokine levels, and dampens angiogenic and neural transcriptomic pathways in the rat 50/10 OIR model. The study identifies a sex-specific transcriptomic response to anemia in the 50/10 OIR model, with females primarily impacted.

Indexed as

AnemiaOxygenPhlebotomyRetinaRetinal NeovascularizationRetinopathy of PrematurityTranscriptomeAnimalsAnimals, NewbornCytokinesDisease Models, AnimalFemaleMaleRatsRats, Sprague-DawleyReceptors, TransferrinCytokinesOxygenReceptors, TransferrinVascular Endothelial Growth Factor A

Identifiers

PMID39379628

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.