Evidence map›Paper›PMID 39379820›Full record

ArticleBMC microbiology2024

Proteomic analysis reveals molecular changes following genetic engineering in Chlamydomonas reinhardtii.

Lorenzo Barolo, Raffaela M Abbriano, Audrey S Commault, Matthew P Padula, Mathieu Pernice

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Lorenzo BaroloUniversity of Technology Sydney, Climate Change Cluster, Broadway Campus, Ultimo, Sydney, NSW, 2007, Australia. lorenzo.barolo@uniroma1.it.
Raffaela M AbbrianoUniversity of Technology Sydney, Climate Change Cluster, Broadway Campus, Ultimo, Sydney, NSW, 2007, Australia.
Audrey S CommaultUniversity of Technology Sydney, Climate Change Cluster, Broadway Campus, Ultimo, Sydney, NSW, 2007, Australia.
Matthew P PadulaSchool of Life Sciences and Proteomics Core Facility, Faculty of Science, University of Technology Sydney, Ultimo, Sydney, NSW, 2007, Australia.
Mathieu PerniceUniversity of Technology Sydney, Climate Change Cluster, Broadway Campus, Ultimo, Sydney, NSW, 2007, Australia. Mathieu.Pernice@uts.edu.au.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundChlamydomonas reinhardtii is gaining recognition as a promising expression system for the production of recombinant proteins. However, its performance as a cellular biofactory remains suboptimal, especially with respect to consistent expression of heterologous genes. Gene silencing mechanisms, position effect, and low nuclear transgene expression are major drawbacks for recombinant protein production in this model system. To unveil the molecular changes following transgene insertion, retention, and expression in this species, we genetically engineered C. reinhardtii wild type strain 137c (strain cc-125 mt+) to express the fluorescent protein mVenus and subsequently analysed its intracellular proteome.

resultsThe obtained transgenic cell lines showed differences in abundance in more than 400 proteins, with multiple pathways altered post-transformation. Proteins involved in chromatin remodelling, translation initiation and elongation, and protein quality control and transport were found in lower abundance. On the other hand, ribosomal proteins showed higher abundance, a signal of ribosomal stress response.

conclusionsThese results provide new insights into the modifications of C. reinhardtii proteome after transformation, highlighting possible pathways involved in gene silencing. Moreover, this study identifies multiple protein targets for future genetic engineering approaches to improve the prospective use of C. reinhardtii as cell biofactory for industrial applications.

Indexed as

Chlamydomonas reinhardtiiGenetic EngineeringProteomeProteomicsGene SilencingRecombinant ProteinsTransgenesProteomeRecombinant ProteinsChlamydomonas reinhardtiiGenetic engineeringMicroalgaeProteome analysisRecombinant proteinTransgene

Identifiers

PMID39379820
PMCPMC11460192

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.