Evidence map›Paper›PMID 39380110›Full record

ArticleJournal of animal science and biotechnology2024

Seasonal influence on miRNA expression dynamics of extracellular vesicles in equine follicular fluid.

Jean M Feugang, Ahmed Gad, Nico G Menjivar, Ghassan M Ishak, Samuel Gebremedhn, Melba O Gastal, Notsile H Dlamini, Radek Prochazka, Eduardo L Gastal, Dawit Tesfaye

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In one paragraph

Article in Journal of animal science and biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Jean M FeugangDepartment of Animal and Dairy Sciences, Mississippi State University, Mississippi State, MS, 39762, USA. j.feugang@msstate.edu.ORCID http://orcid.org/0000-0002-1376-5059
Ahmed GadAnimal Reproduction and Biotechnology Laboratory (ARBL), Department of Biomedical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523, USA.
Nico G MenjivarAnimal Reproduction and Biotechnology Laboratory (ARBL), Department of Biomedical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523, USA.
Ghassan M IshakDepartment of Surgery and Obstetrics, College of Veterinary Medicine, University of Baghdad, Baghdad, 10011, Iraq.
Samuel GebremedhnJ.R. Simplot Company, Kuna, ID, 83634, USA.
Melba O GastalAnimal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, 62901, USA.
Notsile H DlaminiDepartment of Animal and Dairy Sciences, Mississippi State University, Mississippi State, MS, 39762, USA.
Radek ProchazkaInstitute of Animal Physiology and Genetics, Czech Academy of Sciences, Liběchov, 27721, Czech Republic.
Eduardo L GastalAnimal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, 62901, USA.
Dawit TesfayeAnimal Reproduction and Biotechnology Laboratory (ARBL), Department of Biomedical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO, 80523, USA.

Funding

NIH MS-INBRE 5P20GMI03476-19USDA-ARS 58-6066-3-038
6 · The paper itself

Abstract

backgroundOvarian follicular fluid (FF) is a dynamic environment that changes with the seasons, affecting follicle development, ovulation, and oocyte quality. Cells in the follicles release tiny particles called extracellular vesicles (EVs) containing vital regulatory molecules, such as microRNAs (miRNAs). These miRNAs are pivotal in facilitating communication within the follicles through diverse signaling and information transfer forms. EV-coupled miRNA signaling is implicated to be associated with ovarian function, follicle and oocyte growth and response to various environmental insults. Herein, we investigated how seasonal variations directly influence the ovulatory and anovulatory states of ovarian follicles and how are they associated with follicular fluid EV-coupled miRNA dynamics in horses.

resultsUltrasonographic monitoring and follicular fluid aspiration of preovulatory follicles in horses during the anovulatory (spring: non-breeding) and ovulatory (spring, summer, and fall: breeding) seasons and subsequent EV isolation and miRNA profiling identified significant variation in EV-miRNA cargo content. We identified 97 miRNAs with differential expression among the groups and specific clusters of miRNAs involved in the spring transition (miR-149, -200b, -206, -221, -328, and -615) and peak breeding period (including miR-143, -192, -451, -302b, -100, and let-7c). Bioinformatic analyses showed enrichments in various biological functions, e.g., transcription factor activity, transcription and transcription regulation, nucleic acid binding, sequence-specific DNA binding, p53 signaling, and post-translational modifications. Cluster analyses revealed distinct sets of significantly up- and down-regulated miRNAs associated with spring anovulatory (Cluster 1) and summer ovulation-the peak breeding season (Clusters 4 and 6).

conclusionsThe findings from the current study shed light on the dynamics of FF-EV-coupled miRNAs in relation to equine ovulatory and anovulatory seasons, and their roles in understanding the mechanisms involved in seasonal shifts and ovulation during the breeding season warrant further investigation.

Indexed as

Extracellular vesicleFollicle growthFollicular fluidHorseMareOvulationPre-ovulationSeasonal breeding

Identifiers

PMID39380110
PMCPMC11462823

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.