Evidence map›Paper›PMID 39380119›Full record

ArticleClinical epigenetics2024

Differences in the DNA methylome of T cells in adults with asthma of varying severity.

Yixuan Liao, Raymond G Cavalcante, Jonathan B Waller, Furong Deng, Anne M Scruggs, Yvonne J Huang, Ulus Atasoy, Yahong Chen, Steven K Huang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical epigenetics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Yixuan LiaoDepartment of Pulmonary and Critical Care Medicine, Peking University Third Hospital, No.49, Huayuan North Road, Haidian District, Beijing, 100191, China.
Raymond G CavalcanteDepartment of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI, USA.
Jonathan B WallerDivision of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan, 6301 MSRB III, 1150 W Medical Center Dr., Ann Arbor, MI, 48109, USA.
Furong DengDepartment of Occupational and Environmental Health Sciences, School of Public Health, Peking University, Beijing, China.
Anne M ScruggsDivision of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan, 6301 MSRB III, 1150 W Medical Center Dr., Ann Arbor, MI, 48109, USA.
Yvonne J HuangDivision of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan, 6301 MSRB III, 1150 W Medical Center Dr., Ann Arbor, MI, 48109, USA.
Ulus AtasoyDivision of Allergy and Clinical Immunology, Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA.
Yahong Chen *Department of Pulmonary and Critical Care Medicine, Peking University Third Hospital, No.49, Huayuan North Road, Haidian District, Beijing, 100191, China. chenyahong@vip.sina.com.
Steven K Huang *Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan, 6301 MSRB III, 1150 W Medical Center Dr., Ann Arbor, MI, 48109, USA. stehuang@umich.edu.

Funding

The Role of KCNMB1 and the Large Conductance Potassium (BK) Channel in Myofibroblast Differentiation and Pulmonary FibrosisR01HL127203 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI HUANG, STEVEN K · 2015 to 2024
$4.4M
Heterogeneity and Regulation of the DNA Methylome in IPF Mesenchymal CellsR01HL162963 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI STEVEN K HUANG · 2023 to 2026
$2.7M
Michigan Medicine-PKUHSC Joint Institute for Translational and Clinical Research BMU20140481NHLBI NIH HHS R01 HL127203NHLBI NIH HHS R01 HL162963NIH HHS HL127203
6 · The paper itself

Abstract

backgroundDNA methylation plays a critical role in asthma development, but differences in DNA methylation among adults with varying asthma severity are less well-defined.

objectiveTo examine how DNA methylomic patterns differ among adults with asthma based on asthma severity and airway inflammation.

methodsPeripheral blood T cells from 35 adults with asthma in Beijing, China, were serially collected over time (130 samples total) and analyzed for global DNA methylation using the Illumina MethylationEPIC Array. Differential methylation was compared among subjects with varying airway inflammation and severity, as measured by fraction of exhaled nitric oxide, forced expiratory volume in one second (FEV1), and Asthma Control Test (ACT) scores.

resultsSignificant differences in DNA methylation were noted among subjects with different degrees of airway inflammation and asthma severity. These differences in DNA methylation were annotated to genes that were enriched in pathways related to asthma or T cell function and included gene ontology categories related to MHC class II assembly, T cell activation, interleukin (IL)-1, and IL-12. Genes related to P450 drug metabolism, glutathione metabolism, and developmental pathways were also differentially methylated in comparisons between subjects with high vs low FEV1 and ACT. Notable genes that were differentially methylated based on asthma severity included RUNX3, several members of the HLA family, AGT, PTPRC, PTPRJ, and several genes downstream of the JAK2 and TNF signaling pathway.

conclusionThese findings demonstrate how adults with asthma of varying severity possess differences in peripheral blood T cell DNA methylation that contribute to differences in clinical indices of asthma.

Indexed as

AsthmaDNA MethylationSeverity of Illness IndexT-LymphocytesAdultChinaEpigenesis, GeneticEpigenomeFemaleHumansMaleMiddle AgedDNA methylationEpigeneticsFraction of exhaled nitric oxide

Identifiers

PMID39380119
PMCPMC11459694

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.