Evidence mapPaperPMID 39382879Full record

ArticleInvestigative ophthalmology & visual science2024

Fetuin-B Interacts With Insulin Receptor-β and Promotes Insulin Resistance in Retina Cells.

Wenyi Zhang, Xin Wang, Shuwei Tian, Jianming Wang, Aiyi Zhou

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Article in Investigative ophthalmology & visual science, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

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3citing papers in PubMed
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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Wenyi ZhangDepartment of Ophthalmology, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
Xin WangDepartment of Ophthalmology, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
Shuwei TianDepartment of Ophthalmology, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
Jianming WangDepartment of Ophthalmology, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
Aiyi ZhouDepartment of Ophthalmology, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Purpose: The purpose of this study was to investigate the correlation between insulin and Fetuin-B (FETUB) and the influence of FETUB on insulin signaling pathway in diabetic retinopathy (DR). Methods: Enzyme-linked immunosorbent assay (ELISA) was used to analyze FETUB and insulin levels in the serum and aqueous fluid of patients with DR and healthy controls. Quantitative PCR (q-PCR), Western blotting, and ELISA were used to examine FETUB expression in ARPE-19, BV2, and Müller cells under insulin stimulation. Co-immunoprecipitation was used to investigate the interaction of FETUB with insulin receptor-β (IRβ). Insulin resistance (IR)-BV2 and IR-Müller cells were treated with FETUB recombinant protein or FETUB short hairpin RNA (shRNA) to explore the influence of FETUB on insulin signaling pathway in DR. LY294002 (a PI3K pathway inhibitor) was used to determine whether FETUB affects glucose metabolism via the PI3K/Akt pathway. Results: In aqueous fluid, FETUB concentrations were positively correlated with insulin levels. FETUB expression increased in Müller and BV2 cells under insulin regulation, and FETUB interacted with IRβ in retinal cells and mice retina. The interaction between IRβ and FETUB increased in BV2 and Müller cells under high-glucose than in controls. Insulin signaling pathway activation was suppressed in FETUB recombinant protein-treated BV2 and Müller cells but increased in FETUB shRNA-transfected cells. FETUB shRNA could not reverse LY294002-mediated inhibition of glucose transporter-4 expression. Conclusions: Retinal cells are the source of insulin-regulated FETUB. The FETUB interacts with IRβ and affects insulin signaling pathway in BV2 and Müller cells. FETUB may aggravate IR in BV2 and Müller cells via the PI3K/Akt pathway.

Indexed as

Blotting, WesternDiabetic RetinopathyEnzyme-Linked Immunosorbent AssayEpendymoglial CellsFetuin-BInsulinInsulin ResistanceReceptor, InsulinSignal TransductionAnimalsAqueous HumorCells, CulturedFemaleHumansImmunoprecipitationMaleFetuin-BInsulinReceptor, Insulin

Identifiers

PMID39382879
PMCPMC11469143

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.