Evidence map›Paper›PMID 39384175›Full record

ArticleBMB reports2024

Differential roles of N- and C-terminal LIR motifs in the catalytic activity and membrane targeting of RavZ and ATG4B proteins.

Sang-Won Park, Ju-Hui Park, Haneul Choi, Pureum Jeon, Seung-Hwan Lee, Won-Dong Shin, Hun-Joo Kim, Jin-A Lee, Deok-Jin Jang

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In one paragraph

Article in BMB reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Mechanisms and roles of membrane-anchored ATG8s.Frontiers in cell and developmental biology · 2025
    Review
  4. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors.

Sang-Won ParkDepartment of Vector Entomology, College of Ecology and Environment, Kyungpook National University, Sangju 37224; Research Institute of Invertebrate Vector, Kyungpook National University, Sangju 37224, Korea.
Ju-Hui ParkDepartment of Ecological Science, College of Ecology and Environment, Kyungpook National University, Sangju 37224, Korea.
Haneul ChoiDepartment of Biological Sciences and Biotechnology, College of Life Sciences and Nanotechnology, Hannam University, Daejeon 34054, Korea.
Pureum JeonDepartment of Biological Sciences and Biotechnology, College of Life Sciences and Nanotechnology, Hannam University, Daejeon 34054, Korea.
Seung-Hwan LeeDepartment of Ecological Science, College of Ecology and Environment, Kyungpook National University, Sangju 37224, Korea.
Won-Dong ShinDepartment of Vector Entomology, College of Ecology and Environment, Kyungpook National University, Sangju 37224; Research Institute of Invertebrate Vector, Kyungpook National University, Sangju 37224, Korea.
Hun-Joo KimDepartment of Vector Entomology, College of Ecology and Environment, Kyungpook National University, Sangju 37224, Korea.
Jin-A LeeDepartment of Biological Sciences and Biotechnology, College of Life Sciences and Nanotechnology, Hannam University, Daejeon 34054, Korea.
Deok-Jin JangDepartment of Vector Entomology, College of Ecology and Environment, Kyungpook National University, Sangju 37224; Research Institute of Invertebrate Vector, Kyungpook National University, Sangju 37224; Department of Ecological Science, College of Ecology and Environment, Kyungpook National University, Sangju 37224, Korea.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mammalian ATG8 proteins (mATG8s) are essential for selective autophagy because they recruit various proteins with LC3- interacting region (LIR) motifs to autophagic membranes. The RavZ protein, secreted by Legionella pneumophila, and mammalian ATG4B possess functional LIR motifs that participate in lipidated mATG8 deconjugation on autophagic membranes. RavZ comprises three functional LIR motifs at the N- and Cterminal sides of its catalytic domain (CAD). This study demonstrated that LIR motifs at the N-terminal side of the CAD of RavZ are involved in autophagic membrane targeting and substrate recognition, while LIR motif at the C-terminal side facilitate autophagic membrane targeting. Our results also revealed that the C-terminal LIR motif in human ATG4B is pivotal in delipidating LC3B-phosphatidylethanolamine (PE), but it plays a minor role in pro-LC3B priming in the cytosol. Therefore, introducing a functional LIR motif to the N-terminal of ATG4B does not affect LC3B-PE delipidation. This study clearly described the position-dependent roles of LIR motifs in RavZ and ATG4B in cellular contexts. [BMB Reports 2024; 57(11): 497-502].

Indexed as

Amino Acid MotifsAutophagyAutophagy-Related ProteinsCysteine EndopeptidasesMicrotubule-Associated ProteinsPhosphatidylethanolaminesAutophagy-Related Protein 8 FamilyBacterial ProteinsCatalytic DomainCell MembraneHumansLegionella pneumophilaATG4B protein, humanAutophagy-Related Protein 8 FamilyAutophagy-Related ProteinsBacterial ProteinsCysteine EndopeptidasesMAP1LC3B protein, humanMicrotubule-Associated ProteinsphosphatidylethanolaminePhosphatidylethanolamines

Identifiers

PMID39384175
PMCPMC11608851

What Socratic holds

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LicenceCC BY-NC
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.