Evidence map›Paper›PMID 39386450›Full record

ArticlebioRxiv : the preprint server for biology2024

A Genetically Encoded Fluorescent Biosensor for Intracellular Measurement of Malonyl-CoA.

Brodie L Ranzau, Tiffany D Robinson, Jack M Scully, Edmund D Kapelczack, Teagan S Dean, Tara TeSlaa, Danielle L Schmitt

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Brodie L RanzauDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA, USA.
Tiffany D RobinsonDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA, USA.
Jack M ScullyDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA, USA.
Edmund D KapelczackDepartment of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
Teagan S DeanDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA, USA.
Tara TeSlaaDepartment of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, USA.
Danielle L SchmittDepartment of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, CA, USA.

Funding

Women's CancersP30CA016042 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Robert Damoiseaux · 1985 to 2026
$134.5M
UCLA NIGMS T32 Program AssessmentT32GM007185 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI TORRES, JORGE · 1985 to 2020
$21.7M
Imaging Spatiotemporal Regulation of Acetyl-CoADP2GM154012 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Danielle Lynanne Schmitt · 2023 to 2026
$2.2M
NCI NIH HHS P30 CA016042NIGMS NIH HHS DP2 GM154012NIGMS NIH HHS T32 GM007185
6 · The paper itself

Abstract

Malonyl-CoA is the essential building block of fatty acids and regulates cell function through protein malonylation and allosteric regulation of signaling networks. Accordingly, the production and use of malonyl-CoA is finely tuned by the cellular energy status. Most studies of malonyl-CoA dynamics rely on bulk approaches that take only a snapshot of the average metabolic state of a population of cells, missing out on dynamic changes in malonyl-CoA and fatty acid biosynthesis that could be occurring within a single cell. To overcome this limitation, we have developed a genetically encoded fluorescent protein-based biosensor for malonyl-CoA that can be used to capture malonyl-CoA dynamics in single cells. This biosensor, termed Malibu (

Indexed as

biosensorfatty acid biosynthesisfluorescenceMalonyl-CoAmetabolism

Identifiers

PMID39386450
PMCPMC11463626

What Socratic holds

Textmetadata
LicenceCC BY-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.