Evidence map›Paper›PMID 39386678›Full record

ArticlebioRxiv : the preprint server for biology2025

Enhancer AAV toolbox for accessing and perturbing striatal cell types and circuits.

Avery C Hunker, Morgan E Wirthlin, Gursajan Gill, Nelson J Johansen, Marcus Hooper, Victoria Omstead, Sara Vargas, M Nathaly Lerma, Naz Taskin, Natalie Weed and 126 more

Abstract readPreprint
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

136 authors.

Avery C HunkerAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0003-0508-3637
Morgan E WirthlinAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0001-7967-7070
Gursajan GillDepartment of Neurosurgery, Stanford University School of Medicine, Stanford, CA.ORCID 0009-0002-2789-4430
Nelson J JohansenAllen Institute for Brain Science, Seattle, WA.
Marcus HooperAllen Institute for Brain Science, Seattle, WA.
Victoria OmsteadAllen Institute for Brain Science, Seattle, WA.
Sara VargasAllen Institute for Brain Science, Seattle, WA.
M Nathaly LermaAllen Institute for Brain Science, Seattle, WA.
Naz TaskinAllen Institute for Brain Science, Seattle, WA.
Natalie WeedAllen Institute for Brain Science, Seattle, WA.
William D LairdAllen Institute for Brain Science, Seattle, WA.
Yemeserach M BishawAllen Institute for Brain Science, Seattle, WA.
Jacqueline L BendrickDepartment of Neurosurgery, Stanford University School of Medicine, Stanford, CA.ORCID 0000-0002-2694-9914
Bryan B GoreAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0003-1721-4235
Yoav Ben-SimonAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0002-7075-097X
Ximena Opitz-ArayaAllen Institute for Brain Science, Seattle, WA.
Refugio A MartinezAllen Institute for Brain Science, Seattle, WA.
Sharon W WayAllen Institute for Brain Science, Seattle, WA.
Bargavi ThyagarajanAllen Institute for Brain Science, Seattle, WA.
Sven OttoAllen Institute for Brain Science, Seattle, WA.ORCID 0009-0002-8383-4272
Raymond E A SanchezAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0001-9141-090X
Jason R AlexanderAllen Institute for Brain Science, Seattle, WA.ORCID 0009-0000-7591-9925
Avalon AmayaAllen Institute for Neural Dynamics, Seattle, WA.ORCID 0000-0003-2274-2892
Adam AmsterAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0002-5724-7472
Joel ArbuckleAllen Institute for Brain Science, Seattle, WA.
Angela AyalaAllen Institute for Brain Science, Seattle, WA.ORCID 0009-0002-8512-6851
Pam M BakerAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0002-1376-5649
Tyler BarcelliAllen Institute for Brain Science, Seattle, WA.ORCID 0009-0000-0231-1441
Stuard BartaAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0001-7180-3466
Darren BertagnolliAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0002-6626-1567
Cameron BielsteinAllen Institute for Brain Science, Seattle, WA.ORCID 0009-0006-0852-7255
Prajal BishwakarmaAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0002-9802-0493
Jessica BowlusAllen Institute for Brain Science, Seattle, WA.ORCID 0009-0009-7335-3855
Gabriella BoyerAllen Institute for Brain Science, Seattle, WA.ORCID 0009-0007-2146-2078
Krissy BrounerAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0001-5790-7154
Brittny CasianAllen Institute for Brain Science, Seattle, WA.ORCID 0009-0009-9965-0364
Tamara CasperAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0003-1638-3651
Anish Bhaswanth ChakkaAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0002-1095-1817
Rushil ChakrabartyAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0003-1722-8557
Michael ClarkAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0002-8006-4332
Kaity ColbertAllen Institute for Brain Science, Seattle, WA.ORCID 0009-0000-2864-7488
Scott DanielAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0001-8319-2359
Tim DaweAllen Institute for Brain Science, Seattle, WA.
Maxwell DeparteeAllen Institute for Brain Science, Seattle, WA.ORCID 0009-0002-3723-6474
Peter DiValentinAllen Institute for Brain Science, Seattle, WA.ORCID 0009-0003-0987-912X
Nicholas P DonadioAllen Institute for Brain Science, Seattle, WA.
Nadezhda I DotsonAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0003-3414-3176
Deepanjali DwivediAllen Institute for Brain Science, Seattle, WA.
Tom EgdorfAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0001-9059-8904
Tim FlissAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0003-3303-658X
Amanda GaryAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0002-6397-1520
Jeff GoldyAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0001-5140-6922
Conor GrassoAllen Institute for Neural Dynamics, Seattle, WA.ORCID 0009-0003-6384-4075
Erin L GroceAllen Institute for Brain Science, Seattle, WA.
Kathryn GudsnukAllen Institute for Brain Science, Seattle, WA.
Warren HanAllen Institute for Neural Dynamics, Seattle, WA.ORCID 0009-0006-0104-0380
Zeb HaradonAllen Institute for Brain Science, Seattle, WA.
Sam HastingsAllen Institute for Brain Science, Seattle, WA.
Olivia HelbackAllen Institute for Brain Science, Seattle, WA.ORCID 0009-0001-7892-6319
Windy V HoAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0001-6200-9906
Cindy HuangAllen Institute for Brain Science, Seattle, WA.ORCID 0009-0002-5386-6345
Tye JohnsonAllen Institute for Neural Dynamics, Seattle, WA.ORCID 0009-0006-7239-7571
Danielle L JonesAllen Institute for Brain Science, Seattle, WA.
Zoe JuneauAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0002-8860-5260
Jaimie KenneyAllen Institute for Neural Dynamics, Seattle, WA.ORCID 0009-0003-5308-2783
Madison LeiblyAllen Institute for Brain Science, Seattle, WA.ORCID 0009-0000-1277-9400
Su LiAllen Institute for Brain Science, Seattle, WA.
Elizabeth LiangAllen Institute for Brain Science, Seattle, WA.ORCID 0009-0000-1781-6784
Henry LoefflerAllen Institute for Neural Dynamics, Seattle, WA.ORCID 0009-0007-3935-6863
Nicholas A LuskAllen Institute for Brain Science, Seattle, WA.
Zachary MadiganAllen Institute for Brain Science, Seattle, WA.ORCID 0009-0001-4949-9637
Jessica MalloyAllen Institute for Brain Science, Seattle, WA.ORCID 0009-0001-5494-8054
Jocelin MaloneAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0003-0794-4250
Rachel McCueAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0003-4339-7581
Jose MelchorAllen Institute for Brain Science, Seattle, WA.ORCID 0009-0008-9759-0583
John K MichAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0002-1626-1139
Skyler MoosmanAllen Institute for Brain Science, Seattle, WA.
Elyse MorinAllen Institute for Brain Science, Seattle, WA.
Robyn NaidooAllen Institute for Neural Dynamics, Seattle, WA.ORCID 0009-0004-0996-8808
Dakota NewmanAllen Institute for Brain Science, Seattle, WA.
Kiet NgoAllen Institute for Brain Science, Seattle, WA.ORCID 0009-0002-5548-1983
Katrina NguyenAllen Institute for Neural Dynamics, Seattle, WA.ORCID 0009-0009-8547-9703
Aaron L OsterAllen Institute for Brain Science, Seattle, WA.
Ben OuelletteAllen Institute for Neural Dynamics, Seattle, WA.ORCID 0000-0003-4913-554X
Alana A OyamaAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0002-4492-0533
Nick PenaAllen Institute for Brain Science, Seattle, WA.
Trangthanh PhamAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0002-4738-5062
Elliot PhillipsAllen Institute for Brain Science, Seattle, WA.ORCID 0009-0008-2762-6608
Christina PomAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0001-9057-040X
Lydia PotekhinaAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0001-8879-7920
Shea RansfordAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0001-6093-0806
Melissa RedingAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0001-9845-1827
Dean F RetteAllen Institute for Brain Science, Seattle, WA.ORCID 0009-0002-5202-6627
Cade ReynoldsonAllen Institute for Brain Science, Seattle, WA.ORCID 0009-0000-8658-0293
Christine RimorinAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0003-1491-8552
Ana Rios SiglerAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0002-5094-8073
Dana B RochaAllen Institute for Brain Science, Seattle, WA.ORCID 0009-0001-2121-8899
Kara RonellenfitchAllen Institute for Brain Science, Seattle, WA.
Augustin RuizAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0002-8361-5152
Lane SawyerAllen Institute for Brain Science, Seattle, WA.
Josh SevignyAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0002-9297-4800
Nadiya V ShapovalovaAllen Institute for Brain Science, Seattle, WA.
Noah ShepardAllen Institute for Brain Science, Seattle, WA.
Lyudmila ShulgaAllen Institute for Brain Science, Seattle, WA.ORCID 0009-0002-9785-328X
Sherif SolimanAllen Institute for Brain Science, Seattle, WA.
Brian StaatsAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0002-3818-3792
Michael J TaorminaAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0003-4634-4039
Michael TieuAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0001-9286-5623
Yimin WangAllen Institute for Brain Science, Seattle, WA.ORCID 0009-0006-3582-3906
Josh WilkesAllen Institute for Neural Dynamics, Seattle, WA.ORCID 0009-0005-4858-2219
Toren WoodAllen Institute for Brain Science, Seattle, WA.ORCID 0009-0009-7437-4260
Thomas ZhouAllen Institute for Brain Science, Seattle, WA.
Ali WillifordAllen Institute for Neural Dynamics, Seattle, WA.ORCID 0009-0000-7282-7515
Nick DeeAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0002-2831-9254
Tyler MollenkopfAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0002-2899-2879
Lydia NgAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0002-7499-3514
Luke EspositoAllen Institute for Brain Science, Seattle, WA.
Brian KalmbachAllen Institute for Brain Science, Seattle, WA.
Shenqin YaoAllen Institute for Brain Science, Seattle, WA.
Jeanelle ArizaAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0002-0829-7887
Forrest CollmanAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0002-0280-7022
Shoaib MuftiAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0002-8173-3884
Kimberly SmithAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0002-3142-1970
Jack WatersAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0002-2312-4183
Ina ErsingAddgene Watertown, MA.
Marcella PatrickAddgene Watertown, MA.
Hongkui ZengAllen Institute for Brain Science, Seattle, WA.
Ed S LeinAllen Institute for Brain Science, Seattle, WA.
Yoshiko KojimaDepartment of Otolaryngology, Head and Neck Surgery, University of Washington, Seattle, WA.
Greg HorwitzDepartment of Neurobiology & Biophysics, University of Washington, Seattle, WA.
Scott F OwenDepartment of Neurosurgery, Stanford University School of Medicine, Stanford, CA.ORCID 0000-0001-6294-7513
Boaz P LeviAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0002-8346-872X
Tanya L DaigleAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0001-9700-8452
Bosiljka TasicAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0002-6861-4506
Trygve E BakkenAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0003-3373-7386
Jonathan T TingAllen Institute for Brain Science, Seattle, WA.ORCID 0000-0001-8266-0392

Funding

Washington National Primate Research CenterP51OD010425 · OD · UNIVERSITY OF WASHINGTON · PI Mari Ostendorf · 2012 to 2026
$201.8M
Functionally guided adult whole brain cell atlas in human and NHPUM1MH130981 · NIMH · ALLEN INSTITUTE · PI Ed Lein, Hongkui Zeng · 2022 to 2026
$91.9M
Open-Access AAV Toolbox for Basal Ganglia Cell Types and CircuitsUF1MH128339 · NIMH · ALLEN INSTITUTE · PI BAKKEN, TRYGVE, DAIGLE, TANYA LYNN · 2021 to 2021
$7.3M
Multimodal analysis of primate infragranular pyramidal neurons and their modulationR01NS123959 · NINDS · ALLEN INSTITUTE · PI DEMBROW, NIKOLAI C, KALMBACH, BRIAN E. · 2021 to 2025
$2.8M
NIH HHS P51 OD010425NIMH NIH HHS UF1 MH128339NIMH NIH HHS UM1 MH130981NINDS NIH HHS R01 NS123959
6 · The paper itself

Abstract

We present an enhancer AAV toolbox for accessing and perturbing striatal cell types and circuits. Best-in-class vectors were curated for accessing major striatal neuron populations including medium spiny neurons (MSNs), direct and indirect pathway MSNs, as well as Sst-Chodl, Pvalb-Pthlh, and cholinergic interneurons. Specificity was evaluated by multiple modes of molecular validation, three different routes of virus delivery, and with diverse transgene cargos. Importantly, we provide detailed information necessary to achieve reliable cell type specific labeling under different experimental contexts. We demonstrate direct pathway circuit-selective optogenetic perturbation of behavior and multiplex labeling of striatal interneuron types for targeted analysis of cellular features. Lastly, we show conserved

Indexed as

AddgeneAdeno-associated virusbasal gangliacaudoputamencholinergicmacaqueMedium spiny neuronPvalbspiny projection neuronSst-chodlstriatonigralstriatopallidal

Identifiers

PMID39386678
PMCPMC11463465

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.