Evidence map›Paper›PMID 39394174›Full record

ArticleJournal of ovarian research2024

Withaferin A ameliorates ovarian cancer-induced renal damage through the regulation of expression of inflammatory cytokines.

Kusum Kumar, Katherine Bosch, Vasa Vemuri, Nicholas Kratholm, Madhavi Rane, Sham S Kakar

Abstract read
In one paragraph

Article in Journal of ovarian research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
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  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Kusum KumarDeparment of Biology, University of Louisville, Louisville, KY, USA.
Katherine BoschDepartment of Biology and Chemistry, Liberty University, Lynchburg, VA, USA.
Vasa VemuriDeparment of Biology, University of Louisville, Louisville, KY, USA.
Nicholas KratholmDepartment of Physiology, School of Medicine, University of Louisville, 500 South Floyd Street, Louisville, KY, 40202, USA.
Madhavi RaneDepartment of Medicine, Division Nephrology, University of Louisville, Louisville, KY, USA.
Sham S KakarDepartment of Physiology, School of Medicine, University of Louisville, 500 South Floyd Street, Louisville, KY, 40202, USA. sham.kakar@louisville.edu.

Funding

UofL Bridges to Baccalaureate(ULBB)R25GM133328 · NIGMS · UNIVERSITY OF LOUISVILLE · PI DAVIS, RANDALL, KAKAR, SHAM S. · 2019 to 2023
$732k
NIGMS NIH HHS R25 GM133328Sham Kakar NAH R25GM133328
6 · The paper itself

Abstract

backgroundCachexia a multifactorial syndrome is a common sequala in patients with cancer. It varies from 42 to 80% depending upon the oncological stage and is directly responsible for 30% of deaths in these patients. Previous research from our laboratory demonstrated that peritoneal ovarian cancer generated in NSG mice resulted in skeletal and cardiac muscle atrophy - leading to loss of skeletal muscle mass and strength, and cardiac dysfunction (cachexia). Treatment of mice bearing i.p. tumors with withaferin A (WFA) showed reversal of skeletal muscle and cardiac cachexia. The present study is focused on determining effects of peritoneal ovarian tumors on kidney damage and effects of WFA treatment on ameliorating kidney damage.

methodsWe generated intraperitoneal ovarian cancer by injecting female NSG mice with ovarian cancer cell line (A2780). After one week of injecting cancer cells, mice were treated with WFA (4 mg/kg) every third day, for three weeks. After 4 weeks of injection of cancer cells, the mice were sacrificed and various tissues including kidney and blood were collected, snap-frozen in liquid nitrogen, and stored at -80

resultsOur results showed a significant increase in levels of expression of inflammatory cytokine IL-1 β (p < 0.01), IL-6 (p < 0.001), TNF-α (p < 0.001), and other related genes including TRAF6 (p < 0.01), MYD88 (p < 0.01), and GDF-15 (p = 0.005) in tumor-bearing mice compared to controls. Treatment of mice bearing tumors with WFA attenuated the increase in expression of each gene. In addition, our results showed a significant increase in creatinine levels in circulation in tumor-bearing mice compared to control mice. Treatment of tumor-bearing mice with WFA resulted in a significant decrease in plasma creatinine levels compared to tumor-bearing mice.

conclusionsOur results conclude that ovarian tumors in NSG mice caused kidney damage and renal dysfunction, which was effectively ameliorated by WFA treatment, suggesting a protective effect of WFA on kidney injury induced by ovarian cancer.

Indexed as

CytokinesOvarian NeoplasmsWithanolidesAnimalsCachexiaCell Line, TumorDisease Models, AnimalFemaleGrowth Differentiation Factor 15HumansKidneyKidney DiseasesMiceCytokinesGrowth Differentiation Factor 15withaferin AWithanolidesCreatinineInflammatory cytokinesKidney damageOvarian cancer and cachexiaRenal dysfunction

Identifiers

PMID39394174
PMCPMC11468018

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.