Evidence map›Paper›PMID 39395111›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Calcium Imaging in Drosophila.

David V Gazzo, Jeremiah J Zartman

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Imaging Cell Competition in Ex-VivoMethods and protocols · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

David V GazzoDepartment of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN, USA.
Jeremiah J ZartmanDepartment of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN, USA. jzartman@nd.edu.

Funding

Regulation and function of multicellular calcium signaling in epithelial growth and regenerationR35GM124935 · NIGMS · UNIVERSITY OF NOTRE DAME · PI ZARTMAN, JEREMIAH JAMES · 2017 to 2021
$1.9M
National Science Foundation (NSF) #2120200NIGMS NIH HHS R35 GM124935
6 · The paper itself

Abstract

Ex vivo calcium imaging in Drosophila opens an expansive amount of research avenues for the study of live signal propagation through complex tissue. Here, we describe how to isolate Drosophila organs of interest, like the developing wing imaginal disc and larval brain, culture them for extended periods, up to 10 h, and how to image the calcium dynamics occurring within them using genetically encoded biosensors like GCaMP. This protocol enables the study of complex calcium signaling dynamics, which is conserved throughout biology in such processes as cell differentiation and proliferation, immune reactions, wound healing, and cell-to-cell and organ-to-organ communication, among others. These methods also allow pharmacological compounds to be tested to observe effects on calcium dynamics with the applications of target identification and therapeutic development.

Indexed as

CalciumCalcium SignalingAnimalsBiosensing TechniquesBrainDrosophilaDrosophila melanogasterImaginal DiscsLarvaMolecular ImagingWings, AnimalCalciumCalcium dynamicsCalcium imagingDrosophilaEx vivoMicrofluidics

Identifiers

PMID39395111

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.