Evidence map›Paper›PMID 39406683›Full record

ArticleChembiochem : a European journal of chemical biology2024

Multipartite Fluorogenic Sensors for Monitoring Tyrosine Phosphatase Activity.

Daniel T Hansen, Julian Tu, Alison W Bouck, Cheryl L Mathis, Amy M Barrios

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Multipartite Fluorogenic Sensors for Monitoring Tyrosine Phosphatase Activity.Chembiochem : a European journal of chemical biology · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Daniel T HansenDepartment of Medicinal Chemistry, University of Utah College of Pharmacy, Salt Lake City, UT, 84112, USA.
Julian TuDepartment of Medicinal Chemistry, University of Utah College of Pharmacy, Salt Lake City, UT, 84112, USA.
Alison W BouckDepartment of Medicinal Chemistry, University of Utah College of Pharmacy, Salt Lake City, UT, 84112, USA.
Cheryl L MathisDepartment of Medicinal Chemistry, University of Utah College of Pharmacy, Salt Lake City, UT, 84112, USA.
Amy M BarriosDepartment of Medicinal Chemistry, University of Utah College of Pharmacy, Salt Lake City, UT, 84112, USA.ORCID 0000-0003-2739-9261

Funding

Chemical Probes of Protein Tyrosine Phosphatase ActivityR01GM135295 · NIGMS · UNIVERSITY OF UTAH · PI BARRIOS, AMY M · 2019 to 2022
$1.2M
NIGMS NIH HHS R01 GM135295NIH HHS R01GM135295
6 · The paper itself

Abstract

Fluorogenic substrates are essential tools for studying the activity of many enzymes including the protein tyrosine phosphatases (PTPs). Here, we have taken the first step toward the development of genetically encodable sensors for PTP activity using fluorescent and fluorogen-activating proteins. The Fluorescence-Activating and absorption Shifting Tag (FAST) is a small protein that becomes fluorescent upon binding to a small molecule dye. We demonstrate that FAST protein can be used as a sensor for PTP-mediated dephosphorylation of phosphorylated dye molecules. Phosphorylated 4-hydroxybenzylidene rhodanine (pHBR) is not able to bind to the FAST protein and induce fluorescence, but provides a sensitive assay for PTP activity, readily detecting 100 pM concentrations of PTP1B in the presence of FAST with a k

Indexed as

Fluorescent DyesProtein Tyrosine Phosphatase, Non-Receptor Type 1Biosensing TechniquesHumansPhosphorylationProtein Tyrosine PhosphatasesRhodanineFluorescent DyesProtein Tyrosine Phosphatase, Non-Receptor Type 1Protein Tyrosine PhosphatasesPTPN1 protein, humanRhodanineFluorescence assayFluorescent proteinFluorophorePhosphatase

Identifiers

PMID39406683
PMCPMC11664904

What Socratic holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.