Evidence mapPaperPMID 39406930Full record

ArticleScientific reports2024

LncRNA LYPLAL1, miR-204-5p, and SIRT1: novel signatures for risk assessment of diabetic macrovascular complications.

Maysa A Mobasher, Marwa A Shabana, Mousa O Germoush, Najlaa Yousef Abuzinadah, Amir Abd-Elhameed, Shereen A Baioumy, Moataz A ElKot, Marwa M Esawy

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Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Maysa A MobasherDepartment of Pathology, Biochemistry Division, College of Medicine, Jouf University, 72388, Sakaka, Saudi Arabia. mmobasher@ju.edu.sa.
Marwa A ShabanaClinical Pathology Department, Faculty of Human Medicine, Zagazig University, Zagazig, Egypt.
Mousa O GermoushBiology Department, College of Science, Jouf University, Sakakah, Saudi Arabia.
Najlaa Yousef AbuzinadahDepartment of biological science, College of Science, University of Jeddah, 23714, Jeddah, Saudi Arabia.
Amir Abd-ElhameedInternal Medicine Department, Faculty of Human Medicine, Zagazig University, Zagazig, Egypt.
Shereen A BaioumyMicrobiology and Immunology Department, Faculty of Human Medicine, Zagazig University, Zagazig, Egypt.
Moataz A ElKotCardiology Department, Faculty of Human Medicine, Zagazig University, Zagazig, Egypt.
Marwa M EsawyClinical Pathology Department, Faculty of Human Medicine, Zagazig University, Zagazig, Egypt.

Funding

Al Jouf University DGSSR-2023-01-02373
6 · The paper itself

Abstract

Long-term, uncontrolled diabetes mellitus can lead to micro- and macrovascular problems. The protective function of lncRNA LYPLAL1 is to reduce endothelium cell inflammation by upregulating sirtuin 1 (SIRT1) and reducing microRNA (miR)-204-5p. This work attempted to examine the lncRNA LYPLAL1/miR-204-5p/SIRT1 molecules as diagnostic biomarkers for diabetic MVC and to assess their clinical correlations. The study enrolled 32 controls, 32 patients with diabetes alone, and 32 patients with diabetic MVC. RT-qPCR, or quantitative real-time PCR, was utilized to determine the expression levels of lncRNA and miR. SIRT1 was measured by ELISA. When comparing cases with MVC to those without MVC, the lncRNA LYPLAL1 and SIRT1 values were significantly lower. Conversely, patients with MVC had significantly higher miR-204-5p levels than those without MVC. The LncRNA LYPLAL1 performed best in terms of detecting MVC. It attained 90.6% specificity and 96.9% sensitivity. A combination of three markers (lncRNA LYPLAL1, miR-204-5p, and SIRT1) yielded the best accuracy at 98.4%. LYPLAL1 expression appeared to be an independent MVC predictor. Adjusted OR for LYPLAL1 expression was 405 (95% CI: 1.4-1200) (p = 0.039). When we compared cases with MVC to those without MVC, the lncRNA LYPLAL1 and SIRT1 values were significantly lower. Patients with MVC had significantly higher miR-204-5p levels than those without MVC. LYPLAL1 LncRNA demonstrated the best performance characteristics. LncRNA LYPLAL1 expression is an independent predictor of MVC.

Indexed as

MicroRNAsRNA, Long NoncodingSirtuin 1AgedBiomarkersCase-Control StudiesDiabetic AngiopathiesFemaleHumansMaleMiddle AgedRisk AssessmentBiomarkersMicroRNAsMIRN204 microRNA, humanRNA, Long NoncodingSIRT1 protein, humanSirtuin 1DiabetesLncRNA LYPLAL1Macrovascular complicationsMiR-204-5pSIRT1

Identifiers

PMID39406930
PMCPMC11480381

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.