Evidence map›Paper›PMID 39411162›Full record

ArticlebioRxiv : the preprint server for biology2024

Enhanced kinase translocation reporters for simultaneous real-time measurement of PKA, ERK, and Ca

Shang-Jui Tsai, Yijing Gong, Austin Dabbs, Fiddia Zahra, Junhao Xu, Aleksander Geske, Michael J Caterina, Stephen J Gould

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Shang-Jui TsaiDepartment of Neurosurgery, Johns Hopkins University School of Medicine, Baltimore, MD 21205 USA.
Yijing GongDepartment of Neurosurgery, Johns Hopkins University School of Medicine, Baltimore, MD 21205 USA.
Austin DabbsDepartment of Neurosurgery, Johns Hopkins University School of Medicine, Baltimore, MD 21205 USA.
Fiddia ZahraDepartment of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205 USA.
Junhao XuDepartment of Neurosurgery, Johns Hopkins University School of Medicine, Baltimore, MD 21205 USA.
Aleksander GeskeDepartment of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Michael J CaterinaDepartment of Neurosurgery, Johns Hopkins University School of Medicine, Baltimore, MD 21205 USA.
Stephen J GouldDepartment of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205 USA.ORCID 0000-0002-4231-998X

Funding

Functional role and therapeutic targeting of exosomes and extracellular RNA biomarkers in heart failureR35HL150807 · NHLBI · MASSACHUSETTS GENERAL HOSPITAL · PI Saumya Das · 2020 to 2026
$6.7M
Synthetic Clamping of Hyperalgesic SignalingR21NS128599 · NINDS · JOHNS HOPKINS UNIVERSITY · PI CATERINA, MICHAEL J, GOULD, STEPHEN J · 2022 to 2022
$450k
NHLBI NIH HHS R35 HL150807NINDS NIH HHS R21 NS128599
6 · The paper itself

Abstract

Kinase translocation reporters (KTRs) are powerful tools for single-cell measurement of time-integrated kinase activity but suffer from restricted dynamic range and limited sensitivity, particularly in neurons. To address these limitations, we developed enhanced KTRs (eKTRs) for protein kinase A (PKA) and extracellular signal-regulated kinase (ERK) that display high sensitivity, rapid response kinetics, broad dynamic range, cell type-specific tuning, and an ability to detect PKA and ERK activity in primary sensory neurons. Moreover, co-expression of optically separable eKTRs for PKA and ERK revealed the kinetics of expected and unexpected crosstalk between PKA, ERK, protein kinase C, and calcium signaling pathways, demonstrating the utility of eKTRs for live cell monitoring of diverse and interacting signaling pathways. These results open the door to improved live-cell and in vivo measurements of key signaling pathways in neurons, while at the same time demonstrating the importance of KTR size and NLS strength to KTR dynamics.

Identifiers

PMID39411162
PMCPMC11475874

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.