Evidence map›Paper›PMID 39411484›Full record

ArticleFrontiers in cell and developmental biology2024

Differential abundance of microRNAs in seminal plasma extracellular vesicles (EVs) in Sahiwal cattle bull related to male fertility.

Vitika Chauhan, Poonam Kashyap, Jatinder Singh Chera, Ankit Pal, Aditya Patel, Seema Karanwal, Shiva Badrhan, Fanny Josan, Subhash Solanki, Mukesh Bhakat and 2 more

Abstract read
In one paragraph

Article in Frontiers in cell and developmental biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Vitika ChauhanAnimal Genomics Laboratory, Animal Biotechnology Division, National Dairy Research Institute, Karnal, India.
Poonam KashyapAnimal Genomics Laboratory, Animal Biotechnology Division, National Dairy Research Institute, Karnal, India.
Jatinder Singh CheraAnimal Genomics Laboratory, Animal Biotechnology Division, National Dairy Research Institute, Karnal, India.
Ankit PalAnimal Genomics Laboratory, Animal Biotechnology Division, National Dairy Research Institute, Karnal, India.
Aditya PatelAnimal Genomics Laboratory, Animal Biotechnology Division, National Dairy Research Institute, Karnal, India.
Seema KaranwalAnimal Genomics Laboratory, Animal Biotechnology Division, National Dairy Research Institute, Karnal, India.
Shiva BadrhanAnimal Genomics Laboratory, Animal Biotechnology Division, National Dairy Research Institute, Karnal, India.
Fanny JosanAnimal Genomics Laboratory, Animal Biotechnology Division, National Dairy Research Institute, Karnal, India.
Subhash SolankiAnimal Genomics Laboratory, Animal Biotechnology Division, National Dairy Research Institute, Karnal, India.
Mukesh BhakatLivestock Production and Management Division, ICAR- Central Institute of Research on Goat, Mathura, Uttar Pradesh, India.
Tirtha Kumar DattaCentral Institute for Research on Buffaloes, Hisar, Haryana, India.
Rakesh KumarAnimal Genomics Laboratory, Animal Biotechnology Division, National Dairy Research Institute, Karnal, India.

Funding

Gates Foundation INV-008501
6 · The paper itself

Abstract

Sahiwal cattle, known for their high milk yield, are propagated through artificial insemination (AI) using male germplasm, largely contingent on semen quality. Spermatozoa, produced in the testes, carry genetic information and molecular signals essential for successful fertilization. Seminal plasma, in addition to sperm, contains nano-sized lipid-bound extracellular vesicles (SP-EVs) that carry key biomolecules, including fertility-related miRNAs, which are essential for bull fertility. The current study focused on miRNA profiling of SP-EVs from high-fertile (HF) and low-fertile (LF) Sahiwal bulls. SP-EVs were isolated using size exclusion chromatography (SEC) and characterized by dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA). Western blotting detected the EV-specific protein markers TSG101 and CD63. The DLS analysis showed SP-EV sizes of 170-180 nm in HF and 130-140 nm in LF samples. The NTA revealed particle concentrations of 5.76 × 10

Indexed as

bull fertilitymiRNARNA-seqSahiwalseminal plasma extracellular vesicles

Identifiers

PMID39411484
PMCPMC11473417

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.