Evidence map›Paper›PMID 39415310›Full record

ArticleJournal of forensic sciences2025

Development of a forensic DNA research grade test material.

Erica L Romsos, Lisa A Borsuk, Carolyn R Steffen, Sarah Riman, Kevin M Kiesler, Peter M Vallone

Abstract read
In one paragraph

Article in Journal of forensic sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Erica L RomsosApplied Genetics Group, National Institute of Standards and Technology, Gaithersburg, Maryland, USA.
Lisa A BorsukApplied Genetics Group, National Institute of Standards and Technology, Gaithersburg, Maryland, USA.
Carolyn R SteffenApplied Genetics Group, National Institute of Standards and Technology, Gaithersburg, Maryland, USA.
Sarah RimanApplied Genetics Group, National Institute of Standards and Technology, Gaithersburg, Maryland, USA.
Kevin M KieslerApplied Genetics Group, National Institute of Standards and Technology, Gaithersburg, Maryland, USA.
Peter M ValloneApplied Genetics Group, National Institute of Standards and Technology, Gaithersburg, Maryland, USA.

Funding

Intramural NIST DOC 9999-NISTNational Institute of Standards and Technology: Special Programs Office (Forensics Genetics Focus Area)
6 · The paper itself

Abstract

Advancements in forensic DNA typing technology and methods have increased sensitivity and, while beneficial, carry the weight of more challenging profile interpretation. In response, the forensic DNA community has often requested more complex reference materials to address commonly encountered measurement and interpretation issues such as complex DNA mixtures, DNA degradation, and PCR inhibition. The National Institute of Standards and Technology (NIST) released Research Grade Test Material 10235: Forensic DNA Typing Resource Samples to support the forensic DNA community. Components include three single source samples, two degraded samples, and three mixture samples. As part of the Research Grade Test Material (RGTM) process, automated methods for bottling, alternative sample tube types, and the addition of carrier RNA for stabilizing low-quantity samples were investigated. Both internal and external testing demonstrate the stability of the material over time at 4°C through qPCR testing. In the development of a data portal, users have been allowed to anonymously upload results and compare their data with NIST and others. This report describes the preparation and stability of this material.

Indexed as

DNADNA FingerprintingDNA Degradation, NecroticHumansMicrosatellite RepeatsPolymerase Chain ReactionReal-Time Polymerase Chain ReactionReference StandardsSpecimen HandlingDNAforensic DNAPCRquantificationreference materialsSTRtraining samplesvalidation

Identifiers

PMID39415310
PMCPMC11693462

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.