Evidence map›Paper›PMID 39422472›Full record

ReviewmBio2024

A review of virus host factor discovery using CRISPR screening.

Wayne Ren See, Meisam Yousefi, Yaw Shin Ooi

Abstract readReview
In one paragraph

Review in mBio, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Wayne Ren SeeProgram in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore.ORCID 0009-0003-0164-9442
Meisam YousefiProgram in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore.ORCID 0000-0002-7346-803X
Yaw Shin OoiProgram in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore.ORCID 0000-0001-9014-1365

Funding

Duke-NUS Medical School (DukeNUS) Pilot Grant Duke/Duke-NUS/RECA(Pilot)/2019/0047Ministry of Education - Singapore (MOE) MOE-000095-01, MOE-T2EP30123-0008National Research Foundation Singapore (NRF) NRF-MOST Joint Grant (MOH-000928)
6 · The paper itself

Abstract

The emergence of genome-scale forward genetic screening techniques, such as Haploid Genetic screen and clustered regularly interspaced short palindromic repeats (CRISPR) knockout screen has opened new horizons in our understanding of virus infection biology. CRISPR screening has become a popular tool for the discovery of novel host factors for several viruses due to its specificity and efficiency in genome editing. Here, we review how CRISPR screening has revolutionized our understanding of virus-host interactions from scientific and technological viewpoints. A summary of the published screens conducted thus far to uncover virus host factors is presented, highlighting their experimental design and significant findings. We will outline relevant methods for customizing the CRISPR screening process to answer more specific hypotheses and compile a glossary of conducted CRISPR screens to show their design aspects. Furthermore, using flaviviruses and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as examples, we hope to offer a broad-based perspective on the capabilities of CRISPR screening to serve as a reference point to guide future unbiased discovery of virus host factors.

Indexed as

Clustered Regularly Interspaced Short Palindromic RepeatsCRISPR-Cas SystemsSARS-CoV-2AnimalsCOVID-19FlavivirusGene EditingHost Microbial InteractionsHost-Pathogen InteractionsHumansVirusesCRISPR screeningflavivirusfunctional genomicshost factorsSARS-CoV-2virus-host interactions

Identifiers

PMID39422472
PMCPMC11559068

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.