ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2024
Effects of intrauterine extracellular vesicle microRNAs on embryonic gene expression in low-fertility cows.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
8 citing papers in PubMed.
- Seminal Extracellular Vesicles as Molecular Modulators of Male Reproductive Function.Reproduction in domestic animals = Zuchthygiene · 2026Review
- Estradiol and progesterone regulate secretion and metabolite content of extracellular vesicles from immortalized bovine uterine epithelial cells.Scientific reports · 2026Article
- Mediating Cross-Talk: How Uterus-Derived Extracellular Vesicles Influence Early Embryo Development and Implantation.Expert reviews in molecular medicine · 2026Review
- FABP3 Mediates Lipid Droplet Accumulation and Adhesive Capacity in Bovine Endometrial Epithelial Cells via PGEAnimals : an open access journal from MDPI · 2025Article
- Non-Invasive Extracellular Vesicle Biomarkers in Endometriosis, Molecular Signatures Linking Pelvic Inflammation, Oocyte Quality, and IVF OutcomesCurrent issues in molecular biology · 2025Review
- Extracellular vesicles in dairy cattle: research progress and prospects for practical applications.Journal of animal science and biotechnology · 2025Review
- Extracellular vesicles in female reproduction: from basic research to application.Animal reproduction · 2025Review
- Effects of intrauterine extracellular vesicle microRNAs on embryonic gene expression in low-fertility cows.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024Article
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Authors and funding
5 authors.
Funding
Abstract
Embryo survival and pre-implantation development depend on uterine luminal fluid, which is believed to play a role in early embryonic death and infertility in cows. Extracellular vesicles (EVs) in the uterine luminal fluid contain microRNAs (miRNAs), crucial mediators of intercellular communication. miRNAs regulate conceptus-maternal interactions and participate in embryonic development by suppressing gene expression. Therefore, we hypothesized that miRNAs in the intrauterine EVs of low-fertility cows would hinder embryonic survival and development. EVs were collected from the bovine uterine luminal fluid of both normal- and low-fertility cows 7 days post-estrus. Small RNA-sequencing analysis of miRNAs isolated from these EVs identified eight miRNAs that were highly expressed in normal-fertility cows (normal-fertility miRNAs) and eight with elevated expression in low-fertility cows (low-fertility miRNAs). These two sets of miRNAs were transfected into hatched blastocysts via lipofection. RNA-seq following lipofection with low-fertility miRNAs identified 424 differentially expressed genes (DEGs) relative to the control; in contrast, following lipofection with normal-fertility miRNAs, seven DEGs were identified. Pathway analysis of the DEGs identified following lipofection with low-fertility miRNAs revealed substantial enrichment of mitogen-activated protein kinase (MAPK) signaling. Expression of activator protein 1 (AP1) and interferon-tau (IFNT) mRNA was significantly lower in the low-fertility miRNA transfection group than in the control. IFNT is essential for maternal pregnancy recognition. Therefore, miRNAs in intrauterine EVs from low-fertility cows at 7 days post-estrus may inhibit embryo development and suppress IFNT expression by altering MAPK signaling.
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Registered trials
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