Evidence map›Paper›PMID 39451249›Full record

ArticleCells2024

Multispectral Imaging of Collagen, NAD(P)H and Flavin Autofluorescence in Mesenchymal Stem Cells Undergoing Trilineage Differentiation.

Jared M Campbell, Saabah B Mahbub, Ayad G Anwer, Abbas Habibalahi, Stan Gronthos, Sharon Paton, Shane T Grey, Lindsay E Wu, Robert B Gilchrist, Ewa M Goldys

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Jared M CampbellGraduate School of Biomedical Engineering, University of New South Wales, Sydney, NSW 2052, Australia.ORCID 0000-0003-0163-4251
Saabah B MahbubGraduate School of Biomedical Engineering, University of New South Wales, Sydney, NSW 2052, Australia.
Ayad G AnwerGraduate School of Biomedical Engineering, University of New South Wales, Sydney, NSW 2052, Australia.ORCID 0000-0002-2029-1023
Abbas HabibalahiGraduate School of Biomedical Engineering, University of New South Wales, Sydney, NSW 2052, Australia.
Stan GronthosMesenchymal Stem Cell Laboratory, School of Biomedicine, Faculty of Health and Medical Sciences, University of Adelaide, Adelaide, SA 5000, Australia.ORCID 0000-0002-6225-3084
Sharon PatonMesenchymal Stem Cell Laboratory, School of Biomedicine, Faculty of Health and Medical Sciences, University of Adelaide, Adelaide, SA 5000, Australia.
Shane T GreyGarvan Institute of Medical Research, Darlinghurst, NSW 2010, Australia.
Lindsay E WuSchool of Biomedical Sciences, University of New South Wales, Sydney, NSW 2052, Australia.
Robert B GilchristSchool of Clinical Medicine, University of New South Wales, Sydney, NSW 2052, Australia.ORCID 0000-0003-1611-7142
Ewa M GoldysGraduate School of Biomedical Engineering, University of New South Wales, Sydney, NSW 2052, Australia.

Funding

Australian Research Council DP210102960Australian Research COuncil DP170101863Cancer Institute NSW 2021/ECF1291
6 · The paper itself

Abstract

Understanding the molecular mechanisms of differentiation is important for regenerative medicine and developmental biology. This study aims to characterise the role of the glycolysis/oxidative phosphorylation balance as a driver of mesenchymal stem cell (MSC) differentiation. Cells were maintained in normal conditions or stimulated towards the MSC trilineage cell types over 21 days. Multispectral imaging of cell autofluorescence was applied as a non-invasive methodology to continuously image cultures in situ. Spectral signals for collagen, NAD(P)H, and flavins were unmixed. MSCs cultured under chondrogenic conditions exhibited increased collagen levels relative to controls. Following osteogenic induction, MSCs showed increased collagen levels relative to controls during the earlier stages of culture; however, control cells increased their collagen levels as they became confluent. MSCs cultured under adipogenic conditions exhibited lower levels of collagen than controls. The redox ratio (RR; NAD(P)H/flavins) immediately decreased during chondrogenesis, with this early effect persisting throughout the culture compared to control cells, which appeared to increase their RR, similar to osteogenesis. Adipogenesis resulted in a small increase in RR on day 2 relative to control cells, followed by a persistent decrease. Chondrogenic and adipogenic differentiation favoured oxidative phosphorylation, whereas osteogenesis and MSC overgrowth resulted in a glycolytic metabolism. Following consideration of these findings, as well as the diverse reports in the literature, it is concluded that neither enhanced oxidative phosphorylation nor glycolysis are fundamental to the canonical modes of differentiation, and researchers should avoid interpreting shifts as indicating differentiation.

Indexed as

Cell DifferentiationChondrogenesisCollagenMesenchymal Stem CellsNADPAdipogenesisCells, CulturedFlavinsGlycolysisHumansOsteogenesisCollagenFlavinsNADPautofluorescencedifferentiationmesenchymalspectroscopystem cells

Identifiers

PMID39451249
PMCPMC11505937

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.