Evidence map›Paper›PMID 39453382›Full record

ArticleAging cell2025

Neuronal cathepsin S increases neuroinflammation and causes cognitive decline via CX3CL1-CX3CR1 axis and JAK2-STAT3 pathway in aging and Alzheimer's disease.

Pei-Pei Liu, Xiao-Hui Liu, Ming-Jing Ren, Xiao-Tong Liu, Xiao-Qing Shi, Ming-Li Li, Shu-Ang Li, Yang Yang, Dian-Dian Wang, Yue Wu and 8 more

Abstract read
In one paragraph

Article in Aging cell, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Review
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  7. Review
  8. Review
  9. Article
  10. The spleen-brain axis in Alzheimer's disease and related dementias: Integrating immune and metabolic regulation.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2026
    Review
  11. Review
  12. Article
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  14. Review
  15. Article
  16. Review
  17. Article
  18. [ModifiedNan fang yi ke da xue xue bao = Journal of Southern Medical University · 2025
    Article
  19. Cathepsins in Neurological Diseases.International journal of molecular sciences · 2025
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Pei-Pei LiuClinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.ORCID 0000-0002-3897-3671
Xiao-Hui LiuClinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Ming-Jing RenDepartment of Nephropathy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Xiao-Tong LiuDepartment of Clinical Laboratory, The First Hospital of Yongnian District, Hebei, China.
Xiao-Qing ShiDepartment of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Ming-Li LiDepartment of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Shu-Ang LiClinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Yang YangClinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.ORCID 0000-0001-7432-4735
Dian-Dian WangClinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Yue WuClinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Fan-Xiang YinTranslational Medical Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Yan-Hong GuoDepartment of Nephropathy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Run-Zhou YangClinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Meng ChengHenan Branch, Bank of China, Zhengzhou, Henan, China.
Yong-Juan XinDepartment of Child and Adolescent Health, Precision Nutrition Innovation Center, School of Public Health, Zhengzhou University, Zhengzhou, Henan, China.
Jian-Sheng KangClinical Systems Biology Laboratories, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Bing HuangPain and Related Disease Research Laboratory, Shantou University Medical College, Shantou, Guangdong, China.ORCID 0000-0001-5138-1446
Kai-Di RenDepartment of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.

Funding

Basic and Applied Basic Research Foundation of Guangdong Province 2023B1515230008Henan Province Key Research and development and promotion special 242102311036Joint Construction Project of Henan Province 2018020088Medical Science and Technology Research Project of Henan Province SBGJ202103079National Natural Science Foundation of China 32000522National Natural Science Foundation of China 32000855National Natural Science Foundation of China 82204389Natural Science Foundation of Henan Province 202300410420Natural Science Foundation of Henan Province 212300410274
6 · The paper itself

Abstract

Aging is an intricate process involving interactions among multiple factors, which is one of the main risks for chronic diseases, including Alzheimer's disease (AD). As a member of cysteine protease, cathepsin S (CTSS) has been implicated in inflammation across various diseases. Here, we investigated the role of neuronal CTSS in aging and AD started by examining CTSS expression in hippocampus neurons of aging mice and identified a significant increase, which was negatively correlated with recognition abilities. Concurrently, we observed an elevation of CTSS concentration in the serum of elderly people. Transcriptome and fluorescence-activated cell sorting (FACS) results revealed that CTSS overexpression in neurons aggravated brain inflammatory milieu with microglia activation to M1 pro-inflammatory phenotype, activation of chemokine C-X3-C-motif ligand 1 (CX3CL1)-chemokine C-X3-C-motif receptor 1 (CX3CR1) axis and janus kinase 2 (JAK2)-signal transducer and activator of transcription 3 (STAT3) pathway. As CX3CL1 is secreted by neurons and acts on the CX3CR1 in microglia, our results revealed for the first time the role of neuron CTSS in neuron-microglia "crosstalk." Besides, we observed elevated CTSS expression in multiple brain regions of AD patients, including the hippocampus. Utilizing CTSS selective inhibitor, LY3000328, rescued AD-related pathological features in APP/PS1 mice. We further noticed that neuronal CTSS overexpression increased cathepsin B (CTSB) activity, but decreased cathepsin L (CTSL) activity in microglia. Overall, we provide evidence that CTSS can be used as an aging biomarker and plays regulatory roles through modulating neuroinflammation and recognition in aging and AD process.

Indexed as

AgingAlzheimer DiseaseCathepsinsHippocampusNeuroinflammatory DiseasesNeuronsAdultAgedAnimalsBenzopyransBiomarkersCarbamatesCathepsin BCathepsin LChemokine CX3CL1CX3C Chemokine Receptor 1BenzopyransBiomarkersCarbamatesCathepsin BCathepsin Lcathepsin SCathepsinsChemokine CX3CL1Ctsb protein, mouseCtsl protein, mouseCX3C Chemokine Receptor 1CX3CR1 protein, humanJAK2 protein, humanJanus Kinase 2LY3000328STAT3 protein, humanSTAT3 Transcription FactoragingAlzheimer's diseasecathepsin Sneurodegenerative diseaseneuroinflammationrecognition

Identifiers

PMID39453382
PMCPMC11822647

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.